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Cloning of insertion site flanking sequence and construction of transfer DNA insert mutant library in Stylosanthes colletotrichum.

Chen H, Hu C, Yi K, Huang G, Gao J, Zhang S, Zheng J, Liu Q, Xi J - PLoS ONE (2014)

Bottom Line: We selected 1230 transformants, and obtained 23 strains with pathogenic defects, namely, 18 strains with reduced pathogenicity and five strains with lost pathogenicity.The flanking sequence of T-DNA was compared with the Colletotrichum genome by BLAST, and a sequence of 401 bp was found in Contig464 of the Colletotrichum genome.By predicting the function of the flanking sequence, we discovered that T-DNA insertion in the promoter region of the putative gene had 79% homology with the aspartate aminotransferase gene in Magnaporthe oryzae (XP_003719674.1).

View Article: PubMed Central - PubMed

Affiliation: Institute of Tropical Bioscience and Biotechnology, Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.

ABSTRACT
Stylosanthes sp. is the most important forage legume in tropical areas worldwide. Stylosanthes anthracnose, which is mainly caused by Colletotrichum gloeosporioides, is a globally severe disease in stylo production. Little progress has been made in anthracnose molecular pathogenesis research. In this study, Agrobacterium tumefaciens-mediated transformation was used to transform Stylosanthes colletotrichum strain CH008. The major factors of the genetic transformation system of S. colletotrichum were optimized as follows: A. tumefaciens' AGL-1 concentration (OD(600)), 0.8; concentration of Colletotrichum conidium, 1 × 10(6) conidia/mL; acetosyringone concentration, 100 mmol/L; induction time, 6 h; co-culture temperature, 25 °C; and co-culture time, 3 d. Thus, the transformation efficiency was increased to 300-400 transformants per 106 conidia. Based on the optimized system, a mutant library containing 4616 mutants was constructed, from which some mutants were randomly selected for analysis. Results show that the mutants were single copies that could be stably inherited. The growth rate, spore amount, spore germination rate, and appressorium formation rate in some mutants were significantly different from those in the wild-type strain. We then selected the most appropriate method for the preliminary screening and re-screening of each mutant's pathogenic defects. We selected 1230 transformants, and obtained 23 strains with pathogenic defects, namely, 18 strains with reduced pathogenicity and five strains with lost pathogenicity. Thermal asymmetric interlaced PCR was used to identify the transfer DNA (T-DNA) integration site in the mutant that was coded 2430, and a sequence of 476 bp was obtained. The flanking sequence of T-DNA was compared with the Colletotrichum genome by BLAST, and a sequence of 401 bp was found in Contig464 of the Colletotrichum genome. By predicting the function of the flanking sequence, we discovered that T-DNA insertion in the promoter region of the putative gene had 79% homology with the aspartate aminotransferase gene in Magnaporthe oryzae (XP_003719674.1).

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BLAST results of T-DNA flanking sequences in NCBI.
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pone-0111172-g015: BLAST results of T-DNA flanking sequences in NCBI.

Mentions: BLAST was used to compare the sequence with the sequenced Colletotrichum whole genome database (unpublished), and the website ‘The FGENESH Program’ (Softberry Inc., Mount Kisco, NY, USA; http://linux1.Softberry.com/berry.phtml) was used to predict its functions. Therefore, a hypothetical gene in the regional code of the nucleotide sequence was noted. BLAST was subsequently adopted to compare the sequences in NCBI; 401 nt was completely consistent with the partial sequence of Contig464 of the database (Figure 15). T-DNA was a promoter subregion of the predicted gene. The full length of the predicted gene was 1251 bp. The code of the predicted gene was 416 aa, and the amino acid homology between the predicted gene and Magnaporthe oryzae gene (XP_003719674.1) was 79%. This type of gene codes aspartate transaminase. This code may play an important role in the infection process of pathogeny. Insertion of exogenous sections destroyed the gene’s functions, so the mutants exhibited lost pathogenicity.


Cloning of insertion site flanking sequence and construction of transfer DNA insert mutant library in Stylosanthes colletotrichum.

Chen H, Hu C, Yi K, Huang G, Gao J, Zhang S, Zheng J, Liu Q, Xi J - PLoS ONE (2014)

BLAST results of T-DNA flanking sequences in NCBI.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4215998&req=5

pone-0111172-g015: BLAST results of T-DNA flanking sequences in NCBI.
Mentions: BLAST was used to compare the sequence with the sequenced Colletotrichum whole genome database (unpublished), and the website ‘The FGENESH Program’ (Softberry Inc., Mount Kisco, NY, USA; http://linux1.Softberry.com/berry.phtml) was used to predict its functions. Therefore, a hypothetical gene in the regional code of the nucleotide sequence was noted. BLAST was subsequently adopted to compare the sequences in NCBI; 401 nt was completely consistent with the partial sequence of Contig464 of the database (Figure 15). T-DNA was a promoter subregion of the predicted gene. The full length of the predicted gene was 1251 bp. The code of the predicted gene was 416 aa, and the amino acid homology between the predicted gene and Magnaporthe oryzae gene (XP_003719674.1) was 79%. This type of gene codes aspartate transaminase. This code may play an important role in the infection process of pathogeny. Insertion of exogenous sections destroyed the gene’s functions, so the mutants exhibited lost pathogenicity.

Bottom Line: We selected 1230 transformants, and obtained 23 strains with pathogenic defects, namely, 18 strains with reduced pathogenicity and five strains with lost pathogenicity.The flanking sequence of T-DNA was compared with the Colletotrichum genome by BLAST, and a sequence of 401 bp was found in Contig464 of the Colletotrichum genome.By predicting the function of the flanking sequence, we discovered that T-DNA insertion in the promoter region of the putative gene had 79% homology with the aspartate aminotransferase gene in Magnaporthe oryzae (XP_003719674.1).

View Article: PubMed Central - PubMed

Affiliation: Institute of Tropical Bioscience and Biotechnology, Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.

ABSTRACT
Stylosanthes sp. is the most important forage legume in tropical areas worldwide. Stylosanthes anthracnose, which is mainly caused by Colletotrichum gloeosporioides, is a globally severe disease in stylo production. Little progress has been made in anthracnose molecular pathogenesis research. In this study, Agrobacterium tumefaciens-mediated transformation was used to transform Stylosanthes colletotrichum strain CH008. The major factors of the genetic transformation system of S. colletotrichum were optimized as follows: A. tumefaciens' AGL-1 concentration (OD(600)), 0.8; concentration of Colletotrichum conidium, 1 × 10(6) conidia/mL; acetosyringone concentration, 100 mmol/L; induction time, 6 h; co-culture temperature, 25 °C; and co-culture time, 3 d. Thus, the transformation efficiency was increased to 300-400 transformants per 106 conidia. Based on the optimized system, a mutant library containing 4616 mutants was constructed, from which some mutants were randomly selected for analysis. Results show that the mutants were single copies that could be stably inherited. The growth rate, spore amount, spore germination rate, and appressorium formation rate in some mutants were significantly different from those in the wild-type strain. We then selected the most appropriate method for the preliminary screening and re-screening of each mutant's pathogenic defects. We selected 1230 transformants, and obtained 23 strains with pathogenic defects, namely, 18 strains with reduced pathogenicity and five strains with lost pathogenicity. Thermal asymmetric interlaced PCR was used to identify the transfer DNA (T-DNA) integration site in the mutant that was coded 2430, and a sequence of 476 bp was obtained. The flanking sequence of T-DNA was compared with the Colletotrichum genome by BLAST, and a sequence of 401 bp was found in Contig464 of the Colletotrichum genome. By predicting the function of the flanking sequence, we discovered that T-DNA insertion in the promoter region of the putative gene had 79% homology with the aspartate aminotransferase gene in Magnaporthe oryzae (XP_003719674.1).

Show MeSH
Related in: MedlinePlus