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Detection of human papillomavirus type 39 in a seborrheic inclusion cyst of the buttock.

Song DH, Lee SG, Kim DC, Lee JH, Ko GH, Lee JS - Korean J Pathol (2014)

View Article: PubMed Central - PubMed

Affiliation: Departments of Pathology, The Armed Forces Capital Hospital, Seongnam, Korea.

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A seborrheic inclusion cyst (SIC) is a lesion, which has been described as a combination of seborrheic keratosis and an epidermoid cyst (EC)... However, no SIC study has ever used HPV genotyping... Herein, we report the first case of SIC associated with HPV type 39, which we identified using the HPV DNA chip... In addition, squamous eddies, vacuolated cells and parakeratosis were observed in the cyst wall (Fig. 1C)... No significant area of inflammation as might be present in a foreign body reaction was observed in the adjacent soft tissue... Although few data have been published in regards to SIC with HPV, ECs of cutaneous cysts have been relatively well studied in regards to HPV infection... Kim et al. identified the high-risk HPV type 59 in a verrucous cyst of the flank and suggested that closer attention will be needed to characterize the clinical behavior associated with that type of cyst... Therefore, further studies will be required in order to determine the clinical implications of skin lesions associated with high-risk HPV infection... Park et al. described the development of EC in weight-bearing surfaces using three stages: first, a pre-existing wart is implanted into the dermis by weight; second, the implanted portion forms a cyst in the dermis... This theory was also supported by the association of nongenital seborrheic keratosis with HPV infection, which was described by Tardío et al.... In conclusion, we report a case of SIC associated with HPV type 39, which we identified using DNA chip analysis... This is the first report of successful HPV genotyping associated with SIC.

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Related in: MedlinePlus

Human papillomavirus (HPV) DNA chip analysis uses probes against 32 HPV genotypes. HPV genotype 39 is identified. Beta-globin is used as an internal control in two vertical rows with six spots each. Two spots of hybridization control and 12 spots of internal control worked well in our analysis.
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f2-kjpathol-48-5-398: Human papillomavirus (HPV) DNA chip analysis uses probes against 32 HPV genotypes. HPV genotype 39 is identified. Beta-globin is used as an internal control in two vertical rows with six spots each. Two spots of hybridization control and 12 spots of internal control worked well in our analysis.

Mentions: HPV detection and genotyping were performed using the commercial HPV Genotyping Chip Kit provided by the Biomedlab Company (Seoul, Korea). DNA was isolated from the paraffin-embedded tissue using the DNA extraction kit that was supplied as a component of the HPV Genotyping Kit. Target HPV DNA and beta globin (as an internal control) were amplified by polymerase chain reaction (PCR) and labeled with cyanine 3 for signal detection. The amplified cyanine 3-labeled products were mixed with a hybridization buffer and applied to the DNA chip. Hybridization was performed at 60°C for 1 hour, and then the chip was washed for 2 minutes with distilled water and air-dried at room temperature. HPV DNA hybridization was detected using the DNA Chip Scanner (NimbleGen MS200, Roche, Basel, Switzerland). Of the 32 HPV genotypes tested, the HPV genotype 39 probe was hybridized with the DNA amplified from our specimen (Fig. 2).


Detection of human papillomavirus type 39 in a seborrheic inclusion cyst of the buttock.

Song DH, Lee SG, Kim DC, Lee JH, Ko GH, Lee JS - Korean J Pathol (2014)

Human papillomavirus (HPV) DNA chip analysis uses probes against 32 HPV genotypes. HPV genotype 39 is identified. Beta-globin is used as an internal control in two vertical rows with six spots each. Two spots of hybridization control and 12 spots of internal control worked well in our analysis.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4215970&req=5

f2-kjpathol-48-5-398: Human papillomavirus (HPV) DNA chip analysis uses probes against 32 HPV genotypes. HPV genotype 39 is identified. Beta-globin is used as an internal control in two vertical rows with six spots each. Two spots of hybridization control and 12 spots of internal control worked well in our analysis.
Mentions: HPV detection and genotyping were performed using the commercial HPV Genotyping Chip Kit provided by the Biomedlab Company (Seoul, Korea). DNA was isolated from the paraffin-embedded tissue using the DNA extraction kit that was supplied as a component of the HPV Genotyping Kit. Target HPV DNA and beta globin (as an internal control) were amplified by polymerase chain reaction (PCR) and labeled with cyanine 3 for signal detection. The amplified cyanine 3-labeled products were mixed with a hybridization buffer and applied to the DNA chip. Hybridization was performed at 60°C for 1 hour, and then the chip was washed for 2 minutes with distilled water and air-dried at room temperature. HPV DNA hybridization was detected using the DNA Chip Scanner (NimbleGen MS200, Roche, Basel, Switzerland). Of the 32 HPV genotypes tested, the HPV genotype 39 probe was hybridized with the DNA amplified from our specimen (Fig. 2).

View Article: PubMed Central - PubMed

Affiliation: Departments of Pathology, The Armed Forces Capital Hospital, Seongnam, Korea.

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

A seborrheic inclusion cyst (SIC) is a lesion, which has been described as a combination of seborrheic keratosis and an epidermoid cyst (EC)... However, no SIC study has ever used HPV genotyping... Herein, we report the first case of SIC associated with HPV type 39, which we identified using the HPV DNA chip... In addition, squamous eddies, vacuolated cells and parakeratosis were observed in the cyst wall (Fig. 1C)... No significant area of inflammation as might be present in a foreign body reaction was observed in the adjacent soft tissue... Although few data have been published in regards to SIC with HPV, ECs of cutaneous cysts have been relatively well studied in regards to HPV infection... Kim et al. identified the high-risk HPV type 59 in a verrucous cyst of the flank and suggested that closer attention will be needed to characterize the clinical behavior associated with that type of cyst... Therefore, further studies will be required in order to determine the clinical implications of skin lesions associated with high-risk HPV infection... Park et al. described the development of EC in weight-bearing surfaces using three stages: first, a pre-existing wart is implanted into the dermis by weight; second, the implanted portion forms a cyst in the dermis... This theory was also supported by the association of nongenital seborrheic keratosis with HPV infection, which was described by Tardío et al.... In conclusion, we report a case of SIC associated with HPV type 39, which we identified using DNA chip analysis... This is the first report of successful HPV genotyping associated with SIC.

No MeSH data available.


Related in: MedlinePlus