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Preparation of compact agarose cell blocks from the residues of liquid-based cytology samples.

Choi SJ, Choi YI, Kim L, Park IS, Han JY, Kim JM, Chu YC - Korean J Pathol (2014)

Bottom Line: The resulting agarose cell blocks were trimmed and represented on a CMA for high-throughput analysis using immunocytochemical staining.The SurePath residues were effectively and entirely incorporated into compact agarose cell buttons and embedded in paraffin.This agarose-based compact cell block technique enables preparation of high-quality cell blocks by using up the residual SurePath samples without loss of diagnostic material during cell block preparation.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Inha University Hospital, Inha University School of Medicine, Incheon, Korea.

ABSTRACT

Background: Inevitable loss of diagnostic material should be minimized during cell block preparation. We introduce a modified agarose cell block technique that enables the synthesis of compact cell blocks by using the entirety of a cell pellet without the loss of diagnostic material during cell block preparations. The feasibility of this technique is illustrated by high-throughput immunocytochemistry using high-density cell block microarray (CMA).

Methods: The cell pellets of Sure- Path residues were pre-embedded in ultra-low gelling temperature agarose gel and re-embedded in standard agarose gel. They were fixed, processed, and embedded in paraffin using the same method as tissue sample processing. The resulting agarose cell blocks were trimmed and represented on a CMA for high-throughput analysis using immunocytochemical staining.

Results: The SurePath residues were effectively and entirely incorporated into compact agarose cell buttons and embedded in paraffin. Sections of the agarose cell blocks revealed cellularities that correlated well with corresponding SurePath smears and had immunocytochemical features that were sufficient for diagnosis of difficult cases.

Conclusions: This agarose-based compact cell block technique enables preparation of high-quality cell blocks by using up the residual SurePath samples without loss of diagnostic material during cell block preparation.

No MeSH data available.


Related in: MedlinePlus

SurePath smear and agarose cell block from parathyroid lesion. The SurePath sample is allegedly from an intrathyroidal nodule. The smear (A–C) with cohesive clusters and solid sheets of relatively monomorphic epithelial cells was interpreted as “atypia of undetermined significance.” The diagnosis is confirmed by cytoarchitectural examination (D–F) and immunocytochemical analysis (G, thyroglobulin; H, thyroid transcription factor-1; I, parathyroid hormone; arrows indicating positive and negative controls) of cell block sections.
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f7-kjpathol-48-5-351: SurePath smear and agarose cell block from parathyroid lesion. The SurePath sample is allegedly from an intrathyroidal nodule. The smear (A–C) with cohesive clusters and solid sheets of relatively monomorphic epithelial cells was interpreted as “atypia of undetermined significance.” The diagnosis is confirmed by cytoarchitectural examination (D–F) and immunocytochemical analysis (G, thyroglobulin; H, thyroid transcription factor-1; I, parathyroid hormone; arrows indicating positive and negative controls) of cell block sections.

Mentions: The cellularity yield of each cytology spot on the CMA section correlated well with that of the corresponding section of the donor agarose cell blocks (Fig 6A). With the entirety of the diagnostic material concentrated on a viewing cytology spot of each cell block, it was possible to minimize differences in celluarity yield through serial sections of the CMA cut for a series of immunocytochemistry using panels of diagnostic markers (Table 2). Immunocytochemical analyses of the cytology spots on the CMA provided additional information supportive for the diagnosis of cytologically difficult cases (Figs. 6B, 6C, 7).


Preparation of compact agarose cell blocks from the residues of liquid-based cytology samples.

Choi SJ, Choi YI, Kim L, Park IS, Han JY, Kim JM, Chu YC - Korean J Pathol (2014)

SurePath smear and agarose cell block from parathyroid lesion. The SurePath sample is allegedly from an intrathyroidal nodule. The smear (A–C) with cohesive clusters and solid sheets of relatively monomorphic epithelial cells was interpreted as “atypia of undetermined significance.” The diagnosis is confirmed by cytoarchitectural examination (D–F) and immunocytochemical analysis (G, thyroglobulin; H, thyroid transcription factor-1; I, parathyroid hormone; arrows indicating positive and negative controls) of cell block sections.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4215960&req=5

f7-kjpathol-48-5-351: SurePath smear and agarose cell block from parathyroid lesion. The SurePath sample is allegedly from an intrathyroidal nodule. The smear (A–C) with cohesive clusters and solid sheets of relatively monomorphic epithelial cells was interpreted as “atypia of undetermined significance.” The diagnosis is confirmed by cytoarchitectural examination (D–F) and immunocytochemical analysis (G, thyroglobulin; H, thyroid transcription factor-1; I, parathyroid hormone; arrows indicating positive and negative controls) of cell block sections.
Mentions: The cellularity yield of each cytology spot on the CMA section correlated well with that of the corresponding section of the donor agarose cell blocks (Fig 6A). With the entirety of the diagnostic material concentrated on a viewing cytology spot of each cell block, it was possible to minimize differences in celluarity yield through serial sections of the CMA cut for a series of immunocytochemistry using panels of diagnostic markers (Table 2). Immunocytochemical analyses of the cytology spots on the CMA provided additional information supportive for the diagnosis of cytologically difficult cases (Figs. 6B, 6C, 7).

Bottom Line: The resulting agarose cell blocks were trimmed and represented on a CMA for high-throughput analysis using immunocytochemical staining.The SurePath residues were effectively and entirely incorporated into compact agarose cell buttons and embedded in paraffin.This agarose-based compact cell block technique enables preparation of high-quality cell blocks by using up the residual SurePath samples without loss of diagnostic material during cell block preparation.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Inha University Hospital, Inha University School of Medicine, Incheon, Korea.

ABSTRACT

Background: Inevitable loss of diagnostic material should be minimized during cell block preparation. We introduce a modified agarose cell block technique that enables the synthesis of compact cell blocks by using the entirety of a cell pellet without the loss of diagnostic material during cell block preparations. The feasibility of this technique is illustrated by high-throughput immunocytochemistry using high-density cell block microarray (CMA).

Methods: The cell pellets of Sure- Path residues were pre-embedded in ultra-low gelling temperature agarose gel and re-embedded in standard agarose gel. They were fixed, processed, and embedded in paraffin using the same method as tissue sample processing. The resulting agarose cell blocks were trimmed and represented on a CMA for high-throughput analysis using immunocytochemical staining.

Results: The SurePath residues were effectively and entirely incorporated into compact agarose cell buttons and embedded in paraffin. Sections of the agarose cell blocks revealed cellularities that correlated well with corresponding SurePath smears and had immunocytochemical features that were sufficient for diagnosis of difficult cases.

Conclusions: This agarose-based compact cell block technique enables preparation of high-quality cell blocks by using up the residual SurePath samples without loss of diagnostic material during cell block preparation.

No MeSH data available.


Related in: MedlinePlus