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Preparation of compact agarose cell blocks from the residues of liquid-based cytology samples.

Choi SJ, Choi YI, Kim L, Park IS, Han JY, Kim JM, Chu YC - Korean J Pathol (2014)

Bottom Line: The resulting agarose cell blocks were trimmed and represented on a CMA for high-throughput analysis using immunocytochemical staining.The SurePath residues were effectively and entirely incorporated into compact agarose cell buttons and embedded in paraffin.This agarose-based compact cell block technique enables preparation of high-quality cell blocks by using up the residual SurePath samples without loss of diagnostic material during cell block preparation.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Inha University Hospital, Inha University School of Medicine, Incheon, Korea.

ABSTRACT

Background: Inevitable loss of diagnostic material should be minimized during cell block preparation. We introduce a modified agarose cell block technique that enables the synthesis of compact cell blocks by using the entirety of a cell pellet without the loss of diagnostic material during cell block preparations. The feasibility of this technique is illustrated by high-throughput immunocytochemistry using high-density cell block microarray (CMA).

Methods: The cell pellets of Sure- Path residues were pre-embedded in ultra-low gelling temperature agarose gel and re-embedded in standard agarose gel. They were fixed, processed, and embedded in paraffin using the same method as tissue sample processing. The resulting agarose cell blocks were trimmed and represented on a CMA for high-throughput analysis using immunocytochemical staining.

Results: The SurePath residues were effectively and entirely incorporated into compact agarose cell buttons and embedded in paraffin. Sections of the agarose cell blocks revealed cellularities that correlated well with corresponding SurePath smears and had immunocytochemical features that were sufficient for diagnosis of difficult cases.

Conclusions: This agarose-based compact cell block technique enables preparation of high-quality cell blocks by using up the residual SurePath samples without loss of diagnostic material during cell block preparation.

No MeSH data available.


Schematic flow chart of the protocol for preparation of compact agarose cell blocks from the residues of liquid-based cytology samples.
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f1-kjpathol-48-5-351: Schematic flow chart of the protocol for preparation of compact agarose cell blocks from the residues of liquid-based cytology samples.

Mentions: The schematic flow chart of the protocol is represented in Fig. 1.


Preparation of compact agarose cell blocks from the residues of liquid-based cytology samples.

Choi SJ, Choi YI, Kim L, Park IS, Han JY, Kim JM, Chu YC - Korean J Pathol (2014)

Schematic flow chart of the protocol for preparation of compact agarose cell blocks from the residues of liquid-based cytology samples.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4215960&req=5

f1-kjpathol-48-5-351: Schematic flow chart of the protocol for preparation of compact agarose cell blocks from the residues of liquid-based cytology samples.
Mentions: The schematic flow chart of the protocol is represented in Fig. 1.

Bottom Line: The resulting agarose cell blocks were trimmed and represented on a CMA for high-throughput analysis using immunocytochemical staining.The SurePath residues were effectively and entirely incorporated into compact agarose cell buttons and embedded in paraffin.This agarose-based compact cell block technique enables preparation of high-quality cell blocks by using up the residual SurePath samples without loss of diagnostic material during cell block preparation.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Inha University Hospital, Inha University School of Medicine, Incheon, Korea.

ABSTRACT

Background: Inevitable loss of diagnostic material should be minimized during cell block preparation. We introduce a modified agarose cell block technique that enables the synthesis of compact cell blocks by using the entirety of a cell pellet without the loss of diagnostic material during cell block preparations. The feasibility of this technique is illustrated by high-throughput immunocytochemistry using high-density cell block microarray (CMA).

Methods: The cell pellets of Sure- Path residues were pre-embedded in ultra-low gelling temperature agarose gel and re-embedded in standard agarose gel. They were fixed, processed, and embedded in paraffin using the same method as tissue sample processing. The resulting agarose cell blocks were trimmed and represented on a CMA for high-throughput analysis using immunocytochemical staining.

Results: The SurePath residues were effectively and entirely incorporated into compact agarose cell buttons and embedded in paraffin. Sections of the agarose cell blocks revealed cellularities that correlated well with corresponding SurePath smears and had immunocytochemical features that were sufficient for diagnosis of difficult cases.

Conclusions: This agarose-based compact cell block technique enables preparation of high-quality cell blocks by using up the residual SurePath samples without loss of diagnostic material during cell block preparation.

No MeSH data available.