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Histologic disorderliness in the arrangement of tumor cells as an objective measure of tumor differentiation.

Suh S, Park G, Lee YS, Chong Y, Lee YS, Choi YJ - Korean J Pathol (2014)

Bottom Line: We then analyzed a total of 51 photomicrographs of normal colorectal mucosa and colorectal adenocarcinoma with varying degrees of differentiation using our formula.A one-way analysis of variance followed by post hoc pairwise comparisons using Bonferroni correction indicated that the mean disorderliness score was the lowest for the normal colorectal mucosa and increased with decreasing tumor differentiation.Disorderliness, a pathologic feature of malignant tumors that originate from highly organized structures is useful as an objective tumor grading proxy in the field of digital pathology.

View Article: PubMed Central - PubMed

Affiliation: Department of Hospital Pathology, The Catholic University of Korea College of Medicine, Seoul, Korea.

ABSTRACT

Background: Inter-observer and intra-observer variation in histologic tumor grading are well documented. To determine whether histologic disorderliness in the arrangement of tumor cells may serve as an objective criterion for grading, we tested the hypothesis the degree of disorderliness is related to the degree of tumor differentiation on which tumor grading is primarily based.

Methods: Borrowing from the statistical thermodynamic definition of entropy, we defined a novel mathematical formula to compute the relative degree of histologic disorderliness of tumor cells. We then analyzed a total of 51 photomicrographs of normal colorectal mucosa and colorectal adenocarcinoma with varying degrees of differentiation using our formula.

Results: A one-way analysis of variance followed by post hoc pairwise comparisons using Bonferroni correction indicated that the mean disorderliness score was the lowest for the normal colorectal mucosa and increased with decreasing tumor differentiation.

Conclusions: Disorderliness, a pathologic feature of malignant tumors that originate from highly organized structures is useful as an objective tumor grading proxy in the field of digital pathology.

No MeSH data available.


Related in: MedlinePlus

Disorderliness scores of normal colorectal mucosa and a series of simulations of cancerous conditions. (A) Normal colorectal mucosa. (B) Each glandular cell in panel A is converted to a black dot on a white background for semi-automated cell counting; disorderliness score 0.3168. (C) Distorted glands altered from B; disorderliness score 0.3677. (D) A simulation of cell stratification; disorderliness score 0.4286. (E) A simulation of infiltration of tumor cells into the stroma; disorderliness score 0.5434. (F) More diffuse infiltration of tumor cells; disorderliness score 0.7177. Note that the disorderliness score increases steadily as tumor differentiation deceases.
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f1-kjpathol-48-5-339: Disorderliness scores of normal colorectal mucosa and a series of simulations of cancerous conditions. (A) Normal colorectal mucosa. (B) Each glandular cell in panel A is converted to a black dot on a white background for semi-automated cell counting; disorderliness score 0.3168. (C) Distorted glands altered from B; disorderliness score 0.3677. (D) A simulation of cell stratification; disorderliness score 0.4286. (E) A simulation of infiltration of tumor cells into the stroma; disorderliness score 0.5434. (F) More diffuse infiltration of tumor cells; disorderliness score 0.7177. Note that the disorderliness score increases steadily as tumor differentiation deceases.

Mentions: The glandular cells of normal tissue shown in Fig. 1A are represented by black dots on a white background in Fig. 1B, and the disorderliness score was calculated to be 0.3168. Simulated cancerous configurations are illustrated in Figs. 1C through 1F. We first distorted the glands in Fig. 1B to represent a malignant tumor (Fig. 1C). The distorted configuration yielded a disorderliness score 0.3677. We marked some additional dots mimicking cell stratification in the vicinity of the altered glands (Fig. 1D). To simulate infiltration of tumor cells into the stroma, we displaced some of the dots away from the initial location without adding new dots (Fig. 1E) and then placed additional dots to represent a diffuse infiltrative behavior (Fig. 1F). These altered arrangements yielded disorderliness scores of 0.4286, 0.5434 and 0.7177 respectively. Scores were rounded up to the fourth decimal place. The results of the simulations demonstrated that the disorderliness score increased steadily in accordance with decreasing tumor differentiation.


Histologic disorderliness in the arrangement of tumor cells as an objective measure of tumor differentiation.

Suh S, Park G, Lee YS, Chong Y, Lee YS, Choi YJ - Korean J Pathol (2014)

Disorderliness scores of normal colorectal mucosa and a series of simulations of cancerous conditions. (A) Normal colorectal mucosa. (B) Each glandular cell in panel A is converted to a black dot on a white background for semi-automated cell counting; disorderliness score 0.3168. (C) Distorted glands altered from B; disorderliness score 0.3677. (D) A simulation of cell stratification; disorderliness score 0.4286. (E) A simulation of infiltration of tumor cells into the stroma; disorderliness score 0.5434. (F) More diffuse infiltration of tumor cells; disorderliness score 0.7177. Note that the disorderliness score increases steadily as tumor differentiation deceases.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4215958&req=5

f1-kjpathol-48-5-339: Disorderliness scores of normal colorectal mucosa and a series of simulations of cancerous conditions. (A) Normal colorectal mucosa. (B) Each glandular cell in panel A is converted to a black dot on a white background for semi-automated cell counting; disorderliness score 0.3168. (C) Distorted glands altered from B; disorderliness score 0.3677. (D) A simulation of cell stratification; disorderliness score 0.4286. (E) A simulation of infiltration of tumor cells into the stroma; disorderliness score 0.5434. (F) More diffuse infiltration of tumor cells; disorderliness score 0.7177. Note that the disorderliness score increases steadily as tumor differentiation deceases.
Mentions: The glandular cells of normal tissue shown in Fig. 1A are represented by black dots on a white background in Fig. 1B, and the disorderliness score was calculated to be 0.3168. Simulated cancerous configurations are illustrated in Figs. 1C through 1F. We first distorted the glands in Fig. 1B to represent a malignant tumor (Fig. 1C). The distorted configuration yielded a disorderliness score 0.3677. We marked some additional dots mimicking cell stratification in the vicinity of the altered glands (Fig. 1D). To simulate infiltration of tumor cells into the stroma, we displaced some of the dots away from the initial location without adding new dots (Fig. 1E) and then placed additional dots to represent a diffuse infiltrative behavior (Fig. 1F). These altered arrangements yielded disorderliness scores of 0.4286, 0.5434 and 0.7177 respectively. Scores were rounded up to the fourth decimal place. The results of the simulations demonstrated that the disorderliness score increased steadily in accordance with decreasing tumor differentiation.

Bottom Line: We then analyzed a total of 51 photomicrographs of normal colorectal mucosa and colorectal adenocarcinoma with varying degrees of differentiation using our formula.A one-way analysis of variance followed by post hoc pairwise comparisons using Bonferroni correction indicated that the mean disorderliness score was the lowest for the normal colorectal mucosa and increased with decreasing tumor differentiation.Disorderliness, a pathologic feature of malignant tumors that originate from highly organized structures is useful as an objective tumor grading proxy in the field of digital pathology.

View Article: PubMed Central - PubMed

Affiliation: Department of Hospital Pathology, The Catholic University of Korea College of Medicine, Seoul, Korea.

ABSTRACT

Background: Inter-observer and intra-observer variation in histologic tumor grading are well documented. To determine whether histologic disorderliness in the arrangement of tumor cells may serve as an objective criterion for grading, we tested the hypothesis the degree of disorderliness is related to the degree of tumor differentiation on which tumor grading is primarily based.

Methods: Borrowing from the statistical thermodynamic definition of entropy, we defined a novel mathematical formula to compute the relative degree of histologic disorderliness of tumor cells. We then analyzed a total of 51 photomicrographs of normal colorectal mucosa and colorectal adenocarcinoma with varying degrees of differentiation using our formula.

Results: A one-way analysis of variance followed by post hoc pairwise comparisons using Bonferroni correction indicated that the mean disorderliness score was the lowest for the normal colorectal mucosa and increased with decreasing tumor differentiation.

Conclusions: Disorderliness, a pathologic feature of malignant tumors that originate from highly organized structures is useful as an objective tumor grading proxy in the field of digital pathology.

No MeSH data available.


Related in: MedlinePlus