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Direct bio-utilization of untreated rapeseed meal for effective iturin A production by Bacillus subtilis in submerged fermentation.

Jin H, Zhang X, Li K, Niu Y, Guo M, Hu C, Wan X, Gong Y, Huang F - PLoS ONE (2014)

Bottom Line: A significant promoting effect of rapeseed meal on iturin A production was observed and the maximum iturin A concentration of 0.60 g/L was reached at 70 h, which was 20% and 8.0 fold higher than that produced from peptone and ammonium nitrate media, respectively.Moreover, compared to raw rapeseed meal, the remaining residue following fermentation could be used as a more suitable supplementary protein source for animal feed because of the great decrease of major anti-nutritional components including sinapine, glucosinolate and its degradation products of isothiocyanate and oxazolidine thione.The results obtained from this study demonstrate the potential of direct utilization of low cost rapeseed meal as a nitrogen source for commercial production of iturin A and other secondary metabolites by Bacillus subtilis.

View Article: PubMed Central - PubMed

Affiliation: Oil Crops Research Institute, Chinese Academy of Agriculture Sciences, Wuhan, China; Hubei Key Laboratory of Lipid Chemistry and Nutrition, Wuhan, China.

ABSTRACT
The feasibility of using untreated rapeseed meal as a nitrogen source for iturin A production by Bacillus subtilis 3-10 in submerged fermentation was first evaluated by comparison with two different commercial nitrogen sources of peptone and ammonium nitrate. A significant promoting effect of rapeseed meal on iturin A production was observed and the maximum iturin A concentration of 0.60 g/L was reached at 70 h, which was 20% and 8.0 fold higher than that produced from peptone and ammonium nitrate media, respectively. It was shown that rapeseed meal had a positive induction effect on protease secretion, contributing to the release of soluble protein from low water solubility solid rapeseed meal for an effective supply of available nitrogen during fermentation. Moreover, compared to raw rapeseed meal, the remaining residue following fermentation could be used as a more suitable supplementary protein source for animal feed because of the great decrease of major anti-nutritional components including sinapine, glucosinolate and its degradation products of isothiocyanate and oxazolidine thione. The results obtained from this study demonstrate the potential of direct utilization of low cost rapeseed meal as a nitrogen source for commercial production of iturin A and other secondary metabolites by Bacillus subtilis.

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Related in: MedlinePlus

Effects of different initial rapeseed meal concentrations on iturin A production and the initial and final soluble protein concentrations with glucose concentration of 20 g/L in shake flasks.□: initial soluble protein concentration; ▪: final soluble protein concentration. Error bars represent the standard deviation of the means (N = 3).
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pone-0111171-g002: Effects of different initial rapeseed meal concentrations on iturin A production and the initial and final soluble protein concentrations with glucose concentration of 20 g/L in shake flasks.□: initial soluble protein concentration; ▪: final soluble protein concentration. Error bars represent the standard deviation of the means (N = 3).

Mentions: Subsequently, the effect of different initial rapeseed meal concentrations on iturin A production was investigated under the above optimal glucose concentration of 20 g/L. As shown in Figure 2, the optimal initial rapeseed meal concentration for iturin A production was 90 g/L. When the initial concentration of rapeseed meal increased from 30 to 90 g/L, the iturin A concentration increased from 0.22 to 0.45 g/L accordingly; however, the continued increase of initial rapeseed meal from 90 to 150 g/L resulted in the decline of iturin A concentration. Only 0.5–0.8% initial protein was dissolved in the supernatant (Figure 2). Moreover, with the increase of initial rapeseed meal concentrations, the initial soluble protein increased correspondingly (from 0.10 to 0.35 g/L while the initial rapeseed meal increased from 30 to 150 g/L). Further, after 72 hours of fermentation by Bacillus subtilis 3–10, the soluble protein concentration increased significantly compared to the initial concentrations. The soluble protein at 72 h reached 4.8 g/L and increased about 13.7 fold when the initial rapeseed meal concentration was 150 g/L, indicating that a large amount of protein from rapeseed meal were released and dissolved into the medium by the effect of Bacillus subtilis fermentation (Figure 2).


Direct bio-utilization of untreated rapeseed meal for effective iturin A production by Bacillus subtilis in submerged fermentation.

Jin H, Zhang X, Li K, Niu Y, Guo M, Hu C, Wan X, Gong Y, Huang F - PLoS ONE (2014)

Effects of different initial rapeseed meal concentrations on iturin A production and the initial and final soluble protein concentrations with glucose concentration of 20 g/L in shake flasks.□: initial soluble protein concentration; ▪: final soluble protein concentration. Error bars represent the standard deviation of the means (N = 3).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4215929&req=5

pone-0111171-g002: Effects of different initial rapeseed meal concentrations on iturin A production and the initial and final soluble protein concentrations with glucose concentration of 20 g/L in shake flasks.□: initial soluble protein concentration; ▪: final soluble protein concentration. Error bars represent the standard deviation of the means (N = 3).
Mentions: Subsequently, the effect of different initial rapeseed meal concentrations on iturin A production was investigated under the above optimal glucose concentration of 20 g/L. As shown in Figure 2, the optimal initial rapeseed meal concentration for iturin A production was 90 g/L. When the initial concentration of rapeseed meal increased from 30 to 90 g/L, the iturin A concentration increased from 0.22 to 0.45 g/L accordingly; however, the continued increase of initial rapeseed meal from 90 to 150 g/L resulted in the decline of iturin A concentration. Only 0.5–0.8% initial protein was dissolved in the supernatant (Figure 2). Moreover, with the increase of initial rapeseed meal concentrations, the initial soluble protein increased correspondingly (from 0.10 to 0.35 g/L while the initial rapeseed meal increased from 30 to 150 g/L). Further, after 72 hours of fermentation by Bacillus subtilis 3–10, the soluble protein concentration increased significantly compared to the initial concentrations. The soluble protein at 72 h reached 4.8 g/L and increased about 13.7 fold when the initial rapeseed meal concentration was 150 g/L, indicating that a large amount of protein from rapeseed meal were released and dissolved into the medium by the effect of Bacillus subtilis fermentation (Figure 2).

Bottom Line: A significant promoting effect of rapeseed meal on iturin A production was observed and the maximum iturin A concentration of 0.60 g/L was reached at 70 h, which was 20% and 8.0 fold higher than that produced from peptone and ammonium nitrate media, respectively.Moreover, compared to raw rapeseed meal, the remaining residue following fermentation could be used as a more suitable supplementary protein source for animal feed because of the great decrease of major anti-nutritional components including sinapine, glucosinolate and its degradation products of isothiocyanate and oxazolidine thione.The results obtained from this study demonstrate the potential of direct utilization of low cost rapeseed meal as a nitrogen source for commercial production of iturin A and other secondary metabolites by Bacillus subtilis.

View Article: PubMed Central - PubMed

Affiliation: Oil Crops Research Institute, Chinese Academy of Agriculture Sciences, Wuhan, China; Hubei Key Laboratory of Lipid Chemistry and Nutrition, Wuhan, China.

ABSTRACT
The feasibility of using untreated rapeseed meal as a nitrogen source for iturin A production by Bacillus subtilis 3-10 in submerged fermentation was first evaluated by comparison with two different commercial nitrogen sources of peptone and ammonium nitrate. A significant promoting effect of rapeseed meal on iturin A production was observed and the maximum iturin A concentration of 0.60 g/L was reached at 70 h, which was 20% and 8.0 fold higher than that produced from peptone and ammonium nitrate media, respectively. It was shown that rapeseed meal had a positive induction effect on protease secretion, contributing to the release of soluble protein from low water solubility solid rapeseed meal for an effective supply of available nitrogen during fermentation. Moreover, compared to raw rapeseed meal, the remaining residue following fermentation could be used as a more suitable supplementary protein source for animal feed because of the great decrease of major anti-nutritional components including sinapine, glucosinolate and its degradation products of isothiocyanate and oxazolidine thione. The results obtained from this study demonstrate the potential of direct utilization of low cost rapeseed meal as a nitrogen source for commercial production of iturin A and other secondary metabolites by Bacillus subtilis.

Show MeSH
Related in: MedlinePlus