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Direct bio-utilization of untreated rapeseed meal for effective iturin A production by Bacillus subtilis in submerged fermentation.

Jin H, Zhang X, Li K, Niu Y, Guo M, Hu C, Wan X, Gong Y, Huang F - PLoS ONE (2014)

Bottom Line: A significant promoting effect of rapeseed meal on iturin A production was observed and the maximum iturin A concentration of 0.60 g/L was reached at 70 h, which was 20% and 8.0 fold higher than that produced from peptone and ammonium nitrate media, respectively.Moreover, compared to raw rapeseed meal, the remaining residue following fermentation could be used as a more suitable supplementary protein source for animal feed because of the great decrease of major anti-nutritional components including sinapine, glucosinolate and its degradation products of isothiocyanate and oxazolidine thione.The results obtained from this study demonstrate the potential of direct utilization of low cost rapeseed meal as a nitrogen source for commercial production of iturin A and other secondary metabolites by Bacillus subtilis.

View Article: PubMed Central - PubMed

Affiliation: Oil Crops Research Institute, Chinese Academy of Agriculture Sciences, Wuhan, China; Hubei Key Laboratory of Lipid Chemistry and Nutrition, Wuhan, China.

ABSTRACT
The feasibility of using untreated rapeseed meal as a nitrogen source for iturin A production by Bacillus subtilis 3-10 in submerged fermentation was first evaluated by comparison with two different commercial nitrogen sources of peptone and ammonium nitrate. A significant promoting effect of rapeseed meal on iturin A production was observed and the maximum iturin A concentration of 0.60 g/L was reached at 70 h, which was 20% and 8.0 fold higher than that produced from peptone and ammonium nitrate media, respectively. It was shown that rapeseed meal had a positive induction effect on protease secretion, contributing to the release of soluble protein from low water solubility solid rapeseed meal for an effective supply of available nitrogen during fermentation. Moreover, compared to raw rapeseed meal, the remaining residue following fermentation could be used as a more suitable supplementary protein source for animal feed because of the great decrease of major anti-nutritional components including sinapine, glucosinolate and its degradation products of isothiocyanate and oxazolidine thione. The results obtained from this study demonstrate the potential of direct utilization of low cost rapeseed meal as a nitrogen source for commercial production of iturin A and other secondary metabolites by Bacillus subtilis.

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Effects of different initial glucose concentrations on iturin A production and final reducing sugar concentrations at 72 h in shake flasks.▪: reducing sugar concentration. Error bars represent the standard deviation of the means (N = 3).
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pone-0111171-g001: Effects of different initial glucose concentrations on iturin A production and final reducing sugar concentrations at 72 h in shake flasks.▪: reducing sugar concentration. Error bars represent the standard deviation of the means (N = 3).

Mentions: The effect of different initial glucose concentrations ranging from 10 to 70 g/L on iturin A production in shake flasks is in Figure 1. It was clearly demonstrated that untreated rapeseed meal can be used as a nitrogen source for iturin A production. The optimal initial glucose concentration for iturin A production was 20 g/L, and the corresponding iturin A concentration reached 0.45 g/L at 72 h. With the increase of initial glucose concentration from 30 to 70 g/L, the production of iturin A decreased gradually, showing that high initial glucose concentration was not suitable for iturin A production during the entire fermentation period. Interestingly, after 72 h culture, the final reducing sugar concentrations were very similar (about 2 g/L) when the initial glucose was added between 10 and 30 g/L (Figure 1), indicating that the remaining reducing sugars in the supernatant may be unavailable and could not be consumed by Bacillus subtilis 3–10. Moreover, higher initial glucose concentrations (40–70 g/L) resulted in a higher amount of final reducing sugar up to 15 g/L when the initial glucose concentration was 70 g/L (Figure 1).


Direct bio-utilization of untreated rapeseed meal for effective iturin A production by Bacillus subtilis in submerged fermentation.

Jin H, Zhang X, Li K, Niu Y, Guo M, Hu C, Wan X, Gong Y, Huang F - PLoS ONE (2014)

Effects of different initial glucose concentrations on iturin A production and final reducing sugar concentrations at 72 h in shake flasks.▪: reducing sugar concentration. Error bars represent the standard deviation of the means (N = 3).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4215929&req=5

pone-0111171-g001: Effects of different initial glucose concentrations on iturin A production and final reducing sugar concentrations at 72 h in shake flasks.▪: reducing sugar concentration. Error bars represent the standard deviation of the means (N = 3).
Mentions: The effect of different initial glucose concentrations ranging from 10 to 70 g/L on iturin A production in shake flasks is in Figure 1. It was clearly demonstrated that untreated rapeseed meal can be used as a nitrogen source for iturin A production. The optimal initial glucose concentration for iturin A production was 20 g/L, and the corresponding iturin A concentration reached 0.45 g/L at 72 h. With the increase of initial glucose concentration from 30 to 70 g/L, the production of iturin A decreased gradually, showing that high initial glucose concentration was not suitable for iturin A production during the entire fermentation period. Interestingly, after 72 h culture, the final reducing sugar concentrations were very similar (about 2 g/L) when the initial glucose was added between 10 and 30 g/L (Figure 1), indicating that the remaining reducing sugars in the supernatant may be unavailable and could not be consumed by Bacillus subtilis 3–10. Moreover, higher initial glucose concentrations (40–70 g/L) resulted in a higher amount of final reducing sugar up to 15 g/L when the initial glucose concentration was 70 g/L (Figure 1).

Bottom Line: A significant promoting effect of rapeseed meal on iturin A production was observed and the maximum iturin A concentration of 0.60 g/L was reached at 70 h, which was 20% and 8.0 fold higher than that produced from peptone and ammonium nitrate media, respectively.Moreover, compared to raw rapeseed meal, the remaining residue following fermentation could be used as a more suitable supplementary protein source for animal feed because of the great decrease of major anti-nutritional components including sinapine, glucosinolate and its degradation products of isothiocyanate and oxazolidine thione.The results obtained from this study demonstrate the potential of direct utilization of low cost rapeseed meal as a nitrogen source for commercial production of iturin A and other secondary metabolites by Bacillus subtilis.

View Article: PubMed Central - PubMed

Affiliation: Oil Crops Research Institute, Chinese Academy of Agriculture Sciences, Wuhan, China; Hubei Key Laboratory of Lipid Chemistry and Nutrition, Wuhan, China.

ABSTRACT
The feasibility of using untreated rapeseed meal as a nitrogen source for iturin A production by Bacillus subtilis 3-10 in submerged fermentation was first evaluated by comparison with two different commercial nitrogen sources of peptone and ammonium nitrate. A significant promoting effect of rapeseed meal on iturin A production was observed and the maximum iturin A concentration of 0.60 g/L was reached at 70 h, which was 20% and 8.0 fold higher than that produced from peptone and ammonium nitrate media, respectively. It was shown that rapeseed meal had a positive induction effect on protease secretion, contributing to the release of soluble protein from low water solubility solid rapeseed meal for an effective supply of available nitrogen during fermentation. Moreover, compared to raw rapeseed meal, the remaining residue following fermentation could be used as a more suitable supplementary protein source for animal feed because of the great decrease of major anti-nutritional components including sinapine, glucosinolate and its degradation products of isothiocyanate and oxazolidine thione. The results obtained from this study demonstrate the potential of direct utilization of low cost rapeseed meal as a nitrogen source for commercial production of iturin A and other secondary metabolites by Bacillus subtilis.

Show MeSH
Related in: MedlinePlus