Limits...
Use of Cross-Linked Poly(ethylene glycol)-Based Hydrogels for Protein Crystallization.

Gavira JA, Cera-Manjarres A, Ortiz K, Mendez J, Jimenez-Torres JA, Patiño-Lopez LD, Torres-Lugo M - Cryst Growth Des (2014)

Bottom Line: PEG hydrogels also induced the nucleation of lysozyme crystals to a higher extent than agarose.As an example, insulin crystals were grown in 10% (w/w) PEG hydrogel.The resulting crystals were of an approximate size of 500 μm.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de Estudios Crystalográficos, IACT (CSIC-UGR). Avda. las Palmeras 4, E18100 Armilla, Granada, Spain.

ABSTRACT
Poly(ethylene glycol) (PEG) hydrogels are highly biocompatible materials extensively used for biomedical and pharmaceutical applications, controlled drug release, and tissue engineering. In this work, PEG cross-linked hydrogels, synthesized under various conditions, were used to grow lysozyme crystals by the counterdiffusion technique. Crystallization experiments were conducted using a three-layer arrangement. Results demonstrated that PEG fibers were incorporated within lysozyme crystals controlling the final crystal shape. PEG hydrogels also induced the nucleation of lysozyme crystals to a higher extent than agarose. PEG hydrogels can also be used at higher concentrations (20-50% w/w) as a separation chamber (plug) in counterdiffusion experiments. In this case, PEG hydrogels control the diffusion of the crystallization agent and therefore may be used to tailor the supersaturation to fine-tune crystal size. As an example, insulin crystals were grown in 10% (w/w) PEG hydrogel. The resulting crystals were of an approximate size of 500 μm.

No MeSH data available.


Related in: MedlinePlus

Insulin crystals grownin 10% (w/w) PEG hydrogel using the GAMEconfiguration with capillaries of 0.8 mm inner diameter punctuatedin agarose 0.2% (w/v). A and B representing the lower, close to thecrystallization agent insertion point, and the upper parts of thecapillary, respectively.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4215911&req=5

fig7: Insulin crystals grownin 10% (w/w) PEG hydrogel using the GAMEconfiguration with capillaries of 0.8 mm inner diameter punctuatedin agarose 0.2% (w/v). A and B representing the lower, close to thecrystallization agent insertion point, and the upper parts of thecapillary, respectively.

Mentions: Finally, as a proof of concept, we have also tested the useofa PEG hydrogel, a highly biocompatible material, as crystallizationmedia for the production of crystalline insulin–PEG compositematerial. In order to minimize protein consumption, we selected thegel acupuncture method setup of the counterdiffusion techniqueto grow insulin–PEG crystals in capillaries of 0.8 mm innerdiameter. Crystals were observed after 8 days and grown along thecapillary to a final size of approximately 460 × 420 × 420 μm3 (Figure 7).


Use of Cross-Linked Poly(ethylene glycol)-Based Hydrogels for Protein Crystallization.

Gavira JA, Cera-Manjarres A, Ortiz K, Mendez J, Jimenez-Torres JA, Patiño-Lopez LD, Torres-Lugo M - Cryst Growth Des (2014)

Insulin crystals grownin 10% (w/w) PEG hydrogel using the GAMEconfiguration with capillaries of 0.8 mm inner diameter punctuatedin agarose 0.2% (w/v). A and B representing the lower, close to thecrystallization agent insertion point, and the upper parts of thecapillary, respectively.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4215911&req=5

fig7: Insulin crystals grownin 10% (w/w) PEG hydrogel using the GAMEconfiguration with capillaries of 0.8 mm inner diameter punctuatedin agarose 0.2% (w/v). A and B representing the lower, close to thecrystallization agent insertion point, and the upper parts of thecapillary, respectively.
Mentions: Finally, as a proof of concept, we have also tested the useofa PEG hydrogel, a highly biocompatible material, as crystallizationmedia for the production of crystalline insulin–PEG compositematerial. In order to minimize protein consumption, we selected thegel acupuncture method setup of the counterdiffusion techniqueto grow insulin–PEG crystals in capillaries of 0.8 mm innerdiameter. Crystals were observed after 8 days and grown along thecapillary to a final size of approximately 460 × 420 × 420 μm3 (Figure 7).

Bottom Line: PEG hydrogels also induced the nucleation of lysozyme crystals to a higher extent than agarose.As an example, insulin crystals were grown in 10% (w/w) PEG hydrogel.The resulting crystals were of an approximate size of 500 μm.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de Estudios Crystalográficos, IACT (CSIC-UGR). Avda. las Palmeras 4, E18100 Armilla, Granada, Spain.

ABSTRACT
Poly(ethylene glycol) (PEG) hydrogels are highly biocompatible materials extensively used for biomedical and pharmaceutical applications, controlled drug release, and tissue engineering. In this work, PEG cross-linked hydrogels, synthesized under various conditions, were used to grow lysozyme crystals by the counterdiffusion technique. Crystallization experiments were conducted using a three-layer arrangement. Results demonstrated that PEG fibers were incorporated within lysozyme crystals controlling the final crystal shape. PEG hydrogels also induced the nucleation of lysozyme crystals to a higher extent than agarose. PEG hydrogels can also be used at higher concentrations (20-50% w/w) as a separation chamber (plug) in counterdiffusion experiments. In this case, PEG hydrogels control the diffusion of the crystallization agent and therefore may be used to tailor the supersaturation to fine-tune crystal size. As an example, insulin crystals were grown in 10% (w/w) PEG hydrogel. The resulting crystals were of an approximate size of 500 μm.

No MeSH data available.


Related in: MedlinePlus