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Use of Cross-Linked Poly(ethylene glycol)-Based Hydrogels for Protein Crystallization.

Gavira JA, Cera-Manjarres A, Ortiz K, Mendez J, Jimenez-Torres JA, Patiño-Lopez LD, Torres-Lugo M - Cryst Growth Des (2014)

Bottom Line: PEG hydrogels also induced the nucleation of lysozyme crystals to a higher extent than agarose.As an example, insulin crystals were grown in 10% (w/w) PEG hydrogel.The resulting crystals were of an approximate size of 500 μm.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de Estudios Crystalográficos, IACT (CSIC-UGR). Avda. las Palmeras 4, E18100 Armilla, Granada, Spain.

ABSTRACT
Poly(ethylene glycol) (PEG) hydrogels are highly biocompatible materials extensively used for biomedical and pharmaceutical applications, controlled drug release, and tissue engineering. In this work, PEG cross-linked hydrogels, synthesized under various conditions, were used to grow lysozyme crystals by the counterdiffusion technique. Crystallization experiments were conducted using a three-layer arrangement. Results demonstrated that PEG fibers were incorporated within lysozyme crystals controlling the final crystal shape. PEG hydrogels also induced the nucleation of lysozyme crystals to a higher extent than agarose. PEG hydrogels can also be used at higher concentrations (20-50% w/w) as a separation chamber (plug) in counterdiffusion experiments. In this case, PEG hydrogels control the diffusion of the crystallization agent and therefore may be used to tailor the supersaturation to fine-tune crystal size. As an example, insulin crystals were grown in 10% (w/w) PEG hydrogel. The resulting crystals were of an approximate size of 500 μm.

No MeSH data available.


Related in: MedlinePlus

Determination of the mesh size of various PEG plug compositionsemployed to control diffusion of the crystallization agent, obtainedby equilibrium swelling theory. Error bars represent standard errorof the mean of the mesh size (n = 4 for 10%, n = 9 for 20%, n = 8 for 35%, and n = 8 for 50% PEG composition). Statistical differencesare considered when p < 0.05; *p > 0.5 for 20 and 35%.
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fig2: Determination of the mesh size of various PEG plug compositionsemployed to control diffusion of the crystallization agent, obtainedby equilibrium swelling theory. Error bars represent standard errorof the mean of the mesh size (n = 4 for 10%, n = 9 for 20%, n = 8 for 35%, and n = 8 for 50% PEG composition). Statistical differencesare considered when p < 0.05; *p > 0.5 for 20 and 35%.

Mentions: To evaluate all feasibleoperational conditions, we first determined the mesh size (ξ)of the proposed hydrogels at different synthesis conditions. Changesin the mesh size were obtained by adjusting the monomer dilution ratiosand calculated according to eq 4. Figure 2 illustrates the values of the mesh size for eachpolymer composition.


Use of Cross-Linked Poly(ethylene glycol)-Based Hydrogels for Protein Crystallization.

Gavira JA, Cera-Manjarres A, Ortiz K, Mendez J, Jimenez-Torres JA, Patiño-Lopez LD, Torres-Lugo M - Cryst Growth Des (2014)

Determination of the mesh size of various PEG plug compositionsemployed to control diffusion of the crystallization agent, obtainedby equilibrium swelling theory. Error bars represent standard errorof the mean of the mesh size (n = 4 for 10%, n = 9 for 20%, n = 8 for 35%, and n = 8 for 50% PEG composition). Statistical differencesare considered when p < 0.05; *p > 0.5 for 20 and 35%.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4215911&req=5

fig2: Determination of the mesh size of various PEG plug compositionsemployed to control diffusion of the crystallization agent, obtainedby equilibrium swelling theory. Error bars represent standard errorof the mean of the mesh size (n = 4 for 10%, n = 9 for 20%, n = 8 for 35%, and n = 8 for 50% PEG composition). Statistical differencesare considered when p < 0.05; *p > 0.5 for 20 and 35%.
Mentions: To evaluate all feasibleoperational conditions, we first determined the mesh size (ξ)of the proposed hydrogels at different synthesis conditions. Changesin the mesh size were obtained by adjusting the monomer dilution ratiosand calculated according to eq 4. Figure 2 illustrates the values of the mesh size for eachpolymer composition.

Bottom Line: PEG hydrogels also induced the nucleation of lysozyme crystals to a higher extent than agarose.As an example, insulin crystals were grown in 10% (w/w) PEG hydrogel.The resulting crystals were of an approximate size of 500 μm.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de Estudios Crystalográficos, IACT (CSIC-UGR). Avda. las Palmeras 4, E18100 Armilla, Granada, Spain.

ABSTRACT
Poly(ethylene glycol) (PEG) hydrogels are highly biocompatible materials extensively used for biomedical and pharmaceutical applications, controlled drug release, and tissue engineering. In this work, PEG cross-linked hydrogels, synthesized under various conditions, were used to grow lysozyme crystals by the counterdiffusion technique. Crystallization experiments were conducted using a three-layer arrangement. Results demonstrated that PEG fibers were incorporated within lysozyme crystals controlling the final crystal shape. PEG hydrogels also induced the nucleation of lysozyme crystals to a higher extent than agarose. PEG hydrogels can also be used at higher concentrations (20-50% w/w) as a separation chamber (plug) in counterdiffusion experiments. In this case, PEG hydrogels control the diffusion of the crystallization agent and therefore may be used to tailor the supersaturation to fine-tune crystal size. As an example, insulin crystals were grown in 10% (w/w) PEG hydrogel. The resulting crystals were of an approximate size of 500 μm.

No MeSH data available.


Related in: MedlinePlus