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Interaction of human plasma proteins with thin gelatin-based hydrogel films: a QCM-D and ToF-SIMS study.

Schönwälder SM, Bally F, Heinke L, Azucena C, Bulut ÖD, Heißler S, Kirschhöfer F, Gebauer TP, Neffe AT, Lendlein A, Brenner-Weiß G, Lahann J, Welle A, Overhage J, Wöll C - Biomacromolecules (2014)

Bottom Line: This technique enables the determination of adsorbant mass and changes in the shear modulus of the hydrogel layer upon adsorption of human proteins.Furthermore, Secondary Ion Mass Spectrometry and principal component analysis was applied to monitor the changed composition of the topmost adsorbate layer.This approach opens interesting perspectives for a sensitive screening of viscoelastic biomaterials that could be used for regenerative medicine.

View Article: PubMed Central - PubMed

Affiliation: Karlsruhe Institute of Technology (KIT), Institute of Functional Interfaces (IFG) , 76344 Eggenstein-Leopoldshafen, Germany.

ABSTRACT
In the fields of surgery and regenerative medicine, it is crucial to understand the interactions of proteins with the biomaterials used as implants. Protein adsorption directly influences cell-material interactions in vivo and, as a result, regulates, for example, cell adhesion on the surface of the implant. Therefore, the development of suitable analytical techniques together with well-defined model systems allowing for the detection, characterization, and quantification of protein adsorbates is essential. In this study, a protocol for the deposition of highly stable, thin gelatin-based films on various substrates has been developed. The hydrogel films were characterized morphologically and chemically. Due to the obtained low thickness of the hydrogel layer, this setup allowed for a quantitative study on the interaction of human proteins (albumin and fibrinogen) with the hydrogel by Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D). This technique enables the determination of adsorbant mass and changes in the shear modulus of the hydrogel layer upon adsorption of human proteins. Furthermore, Secondary Ion Mass Spectrometry and principal component analysis was applied to monitor the changed composition of the topmost adsorbate layer. This approach opens interesting perspectives for a sensitive screening of viscoelastic biomaterials that could be used for regenerative medicine.

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ΔDn/(−Δfn) values during the adsorptionprocess of Fbn on the gelatin-based hydrogel (blue shades; squares),on bare gold (gray shades; circles), and on the CVD polymer (magentashades; triangles). The corresponding ΔDn/(−Δfn) plot for HSA is shown in the Supporting Information, Figure SI-3. For the sake of clarity,only the third, fifth, and seventh resonance frequencies are shown.
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fig6: ΔDn/(−Δfn) values during the adsorptionprocess of Fbn on the gelatin-based hydrogel (blue shades; squares),on bare gold (gray shades; circles), and on the CVD polymer (magentashades; triangles). The corresponding ΔDn/(−Δfn) plot for HSA is shown in the Supporting Information, Figure SI-3. For the sake of clarity,only the third, fifth, and seventh resonance frequencies are shown.

Mentions: In Figure 6, the dissipation shiftis plottedversus the frequency shift. A linear relationship between the dissipationshift and the frequency shift is clearly visible. For all samples,the factors of proportionality, ΔDn/(−Δfn), for the fifth overtone are shown in Table 1. This linear relationship allows the determination of theshear modulus (Gf) ofthe thin adsorbed protein layer, as described by eq 1 (see Supporting Information(54)):1


Interaction of human plasma proteins with thin gelatin-based hydrogel films: a QCM-D and ToF-SIMS study.

Schönwälder SM, Bally F, Heinke L, Azucena C, Bulut ÖD, Heißler S, Kirschhöfer F, Gebauer TP, Neffe AT, Lendlein A, Brenner-Weiß G, Lahann J, Welle A, Overhage J, Wöll C - Biomacromolecules (2014)

ΔDn/(−Δfn) values during the adsorptionprocess of Fbn on the gelatin-based hydrogel (blue shades; squares),on bare gold (gray shades; circles), and on the CVD polymer (magentashades; triangles). The corresponding ΔDn/(−Δfn) plot for HSA is shown in the Supporting Information, Figure SI-3. For the sake of clarity,only the third, fifth, and seventh resonance frequencies are shown.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4215905&req=5

fig6: ΔDn/(−Δfn) values during the adsorptionprocess of Fbn on the gelatin-based hydrogel (blue shades; squares),on bare gold (gray shades; circles), and on the CVD polymer (magentashades; triangles). The corresponding ΔDn/(−Δfn) plot for HSA is shown in the Supporting Information, Figure SI-3. For the sake of clarity,only the third, fifth, and seventh resonance frequencies are shown.
Mentions: In Figure 6, the dissipation shiftis plottedversus the frequency shift. A linear relationship between the dissipationshift and the frequency shift is clearly visible. For all samples,the factors of proportionality, ΔDn/(−Δfn), for the fifth overtone are shown in Table 1. This linear relationship allows the determination of theshear modulus (Gf) ofthe thin adsorbed protein layer, as described by eq 1 (see Supporting Information(54)):1

Bottom Line: This technique enables the determination of adsorbant mass and changes in the shear modulus of the hydrogel layer upon adsorption of human proteins.Furthermore, Secondary Ion Mass Spectrometry and principal component analysis was applied to monitor the changed composition of the topmost adsorbate layer.This approach opens interesting perspectives for a sensitive screening of viscoelastic biomaterials that could be used for regenerative medicine.

View Article: PubMed Central - PubMed

Affiliation: Karlsruhe Institute of Technology (KIT), Institute of Functional Interfaces (IFG) , 76344 Eggenstein-Leopoldshafen, Germany.

ABSTRACT
In the fields of surgery and regenerative medicine, it is crucial to understand the interactions of proteins with the biomaterials used as implants. Protein adsorption directly influences cell-material interactions in vivo and, as a result, regulates, for example, cell adhesion on the surface of the implant. Therefore, the development of suitable analytical techniques together with well-defined model systems allowing for the detection, characterization, and quantification of protein adsorbates is essential. In this study, a protocol for the deposition of highly stable, thin gelatin-based films on various substrates has been developed. The hydrogel films were characterized morphologically and chemically. Due to the obtained low thickness of the hydrogel layer, this setup allowed for a quantitative study on the interaction of human proteins (albumin and fibrinogen) with the hydrogel by Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D). This technique enables the determination of adsorbant mass and changes in the shear modulus of the hydrogel layer upon adsorption of human proteins. Furthermore, Secondary Ion Mass Spectrometry and principal component analysis was applied to monitor the changed composition of the topmost adsorbate layer. This approach opens interesting perspectives for a sensitive screening of viscoelastic biomaterials that could be used for regenerative medicine.

Show MeSH
Related in: MedlinePlus