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LIN28B promotes colon cancer migration and recurrence.

Pang M, Wu G, Hou X, Hou N, Liang L, Jia G, Shuai P, Luo B, Wang K, Li G - PLoS ONE (2014)

Bottom Line: LIN28B is involved in "stemness" and tumourigenesis by negatively regulating the maturation of let-7 microRNA family members.LIN28B was upregulated in colon cancer tissue compared to normal mucosa, and its overexpression correlated with reduced patient survival and increased tumour recurrence.In conclusion, LIN28B overexpression contributes to colon tumourigenesis, and LIN28B may serve as a diagnostic tool and therapeutic target for colon cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, People's Republic of China.

ABSTRACT
LIN28B is involved in "stemness" and tumourigenesis by negatively regulating the maturation of let-7 microRNA family members. In this study, we showed that LIN28B expression promotes migration and recurrence of colon cancer. Immunohistochemistry and reverse-transcription polymerase chain reactions were performed to detect LIN28B expression in colon cancer tissue microarrays, paraffin-embedded surgical resected tissues and cancer cells. Loss-of-function, migration and proliferation analyses were performed to delineate the potential roles of LIN28B in colon cancer. LIN28B was upregulated in colon cancer tissue compared to normal mucosa, and its overexpression correlated with reduced patient survival and increased tumour recurrence. LIN28B suppression inhibited the migration of SW480 colon cancer cells and facilitated the cytotoxicity induced by oxaliplatin in SW480 and HCT116 colon cancer cells. In conclusion, LIN28B overexpression contributes to colon tumourigenesis, and LIN28B may serve as a diagnostic tool and therapeutic target for colon cancer.

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Silencing of LIN28B suppressed the migration of SW480 cells.The cells were transfected with 50 nM NC or si-LIN28B and were allowed to migrate through a Transwell chamber. Representative graphs are presented.
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pone-0109169-g005: Silencing of LIN28B suppressed the migration of SW480 cells.The cells were transfected with 50 nM NC or si-LIN28B and were allowed to migrate through a Transwell chamber. Representative graphs are presented.

Mentions: As LIN28B overexpression was correlated with tumour recurrence and patient survival, we next sought to explore whether LIN28B expression influences the migration of colon cancer cells. We knocked down LIN28B expression using siRNA, and the Transwell analysis indicated that suppression of LIN28B significantly inhibited the migration of SW480 cells (Fig. 5).


LIN28B promotes colon cancer migration and recurrence.

Pang M, Wu G, Hou X, Hou N, Liang L, Jia G, Shuai P, Luo B, Wang K, Li G - PLoS ONE (2014)

Silencing of LIN28B suppressed the migration of SW480 cells.The cells were transfected with 50 nM NC or si-LIN28B and were allowed to migrate through a Transwell chamber. Representative graphs are presented.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4215835&req=5

pone-0109169-g005: Silencing of LIN28B suppressed the migration of SW480 cells.The cells were transfected with 50 nM NC or si-LIN28B and were allowed to migrate through a Transwell chamber. Representative graphs are presented.
Mentions: As LIN28B overexpression was correlated with tumour recurrence and patient survival, we next sought to explore whether LIN28B expression influences the migration of colon cancer cells. We knocked down LIN28B expression using siRNA, and the Transwell analysis indicated that suppression of LIN28B significantly inhibited the migration of SW480 cells (Fig. 5).

Bottom Line: LIN28B is involved in "stemness" and tumourigenesis by negatively regulating the maturation of let-7 microRNA family members.LIN28B was upregulated in colon cancer tissue compared to normal mucosa, and its overexpression correlated with reduced patient survival and increased tumour recurrence.In conclusion, LIN28B overexpression contributes to colon tumourigenesis, and LIN28B may serve as a diagnostic tool and therapeutic target for colon cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, People's Republic of China.

ABSTRACT
LIN28B is involved in "stemness" and tumourigenesis by negatively regulating the maturation of let-7 microRNA family members. In this study, we showed that LIN28B expression promotes migration and recurrence of colon cancer. Immunohistochemistry and reverse-transcription polymerase chain reactions were performed to detect LIN28B expression in colon cancer tissue microarrays, paraffin-embedded surgical resected tissues and cancer cells. Loss-of-function, migration and proliferation analyses were performed to delineate the potential roles of LIN28B in colon cancer. LIN28B was upregulated in colon cancer tissue compared to normal mucosa, and its overexpression correlated with reduced patient survival and increased tumour recurrence. LIN28B suppression inhibited the migration of SW480 colon cancer cells and facilitated the cytotoxicity induced by oxaliplatin in SW480 and HCT116 colon cancer cells. In conclusion, LIN28B overexpression contributes to colon tumourigenesis, and LIN28B may serve as a diagnostic tool and therapeutic target for colon cancer.

Show MeSH
Related in: MedlinePlus