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LIN28B promotes colon cancer migration and recurrence.

Pang M, Wu G, Hou X, Hou N, Liang L, Jia G, Shuai P, Luo B, Wang K, Li G - PLoS ONE (2014)

Bottom Line: LIN28B is involved in "stemness" and tumourigenesis by negatively regulating the maturation of let-7 microRNA family members.LIN28B was upregulated in colon cancer tissue compared to normal mucosa, and its overexpression correlated with reduced patient survival and increased tumour recurrence.In conclusion, LIN28B overexpression contributes to colon tumourigenesis, and LIN28B may serve as a diagnostic tool and therapeutic target for colon cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, People's Republic of China.

ABSTRACT
LIN28B is involved in "stemness" and tumourigenesis by negatively regulating the maturation of let-7 microRNA family members. In this study, we showed that LIN28B expression promotes migration and recurrence of colon cancer. Immunohistochemistry and reverse-transcription polymerase chain reactions were performed to detect LIN28B expression in colon cancer tissue microarrays, paraffin-embedded surgical resected tissues and cancer cells. Loss-of-function, migration and proliferation analyses were performed to delineate the potential roles of LIN28B in colon cancer. LIN28B was upregulated in colon cancer tissue compared to normal mucosa, and its overexpression correlated with reduced patient survival and increased tumour recurrence. LIN28B suppression inhibited the migration of SW480 colon cancer cells and facilitated the cytotoxicity induced by oxaliplatin in SW480 and HCT116 colon cancer cells. In conclusion, LIN28B overexpression contributes to colon tumourigenesis, and LIN28B may serve as a diagnostic tool and therapeutic target for colon cancer.

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LIN28B is significantly overexpressed in colon tumour tissues.IHC showed that LIN28B was markedly upregulated in tumour tissues compared with the normal mucosa, which demonstrated very little LIN28B expression. Representative graphs are presented.
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pone-0109169-g001: LIN28B is significantly overexpressed in colon tumour tissues.IHC showed that LIN28B was markedly upregulated in tumour tissues compared with the normal mucosa, which demonstrated very little LIN28B expression. Representative graphs are presented.

Mentions: To examine the potential roles for LIN28B in colon cancer, we first compared LIN28B protein expression between 208 tumour samples and 8 non-matched normal mucosa samples from a tissue microarray using IHC. The staining intensity of LIN28B was scored by two pathologists who were blinded to the clinical information. Consistent with previous reports [6], [16], LIN28B was significantly overexpressed in tumour tissues compared to normal tissues (Fig. 1, p<0.001).


LIN28B promotes colon cancer migration and recurrence.

Pang M, Wu G, Hou X, Hou N, Liang L, Jia G, Shuai P, Luo B, Wang K, Li G - PLoS ONE (2014)

LIN28B is significantly overexpressed in colon tumour tissues.IHC showed that LIN28B was markedly upregulated in tumour tissues compared with the normal mucosa, which demonstrated very little LIN28B expression. Representative graphs are presented.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4215835&req=5

pone-0109169-g001: LIN28B is significantly overexpressed in colon tumour tissues.IHC showed that LIN28B was markedly upregulated in tumour tissues compared with the normal mucosa, which demonstrated very little LIN28B expression. Representative graphs are presented.
Mentions: To examine the potential roles for LIN28B in colon cancer, we first compared LIN28B protein expression between 208 tumour samples and 8 non-matched normal mucosa samples from a tissue microarray using IHC. The staining intensity of LIN28B was scored by two pathologists who were blinded to the clinical information. Consistent with previous reports [6], [16], LIN28B was significantly overexpressed in tumour tissues compared to normal tissues (Fig. 1, p<0.001).

Bottom Line: LIN28B is involved in "stemness" and tumourigenesis by negatively regulating the maturation of let-7 microRNA family members.LIN28B was upregulated in colon cancer tissue compared to normal mucosa, and its overexpression correlated with reduced patient survival and increased tumour recurrence.In conclusion, LIN28B overexpression contributes to colon tumourigenesis, and LIN28B may serve as a diagnostic tool and therapeutic target for colon cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, People's Republic of China.

ABSTRACT
LIN28B is involved in "stemness" and tumourigenesis by negatively regulating the maturation of let-7 microRNA family members. In this study, we showed that LIN28B expression promotes migration and recurrence of colon cancer. Immunohistochemistry and reverse-transcription polymerase chain reactions were performed to detect LIN28B expression in colon cancer tissue microarrays, paraffin-embedded surgical resected tissues and cancer cells. Loss-of-function, migration and proliferation analyses were performed to delineate the potential roles of LIN28B in colon cancer. LIN28B was upregulated in colon cancer tissue compared to normal mucosa, and its overexpression correlated with reduced patient survival and increased tumour recurrence. LIN28B suppression inhibited the migration of SW480 colon cancer cells and facilitated the cytotoxicity induced by oxaliplatin in SW480 and HCT116 colon cancer cells. In conclusion, LIN28B overexpression contributes to colon tumourigenesis, and LIN28B may serve as a diagnostic tool and therapeutic target for colon cancer.

Show MeSH
Related in: MedlinePlus