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Glycan analysis of Fonsecaea monophora from clinical and environmental origins reveals different structural profile and human antigenic response.

Burjack JR, Santana-Filho AP, Ruthes AC, Riter DS, Vicente VA, Alvarenga LM, Sassaki GL - Front Cell Infect Microbiol (2014)

Bottom Line: In nature they are found mainly as soil microbiota or decomposing organic matter, and are spread in tropical and subtropical regions.The antigen which contained the cell wall of MMHC82 grown in MM had β-D-Manp units that promoted higher antigenic response.The distribution of these fungal species in nature and the knowledge of how cell wall polysaccharides and glycoconjugates structure vary, may contribute to the better understanding and the elucidation of the pathology caused by this fungus.

View Article: PubMed Central - PubMed

Affiliation: Química de Carboidratos, Departamento de Patologia Básica, Universidade Federal do Paraná Curitiba, Brazil.

ABSTRACT
Dematiaceous fungi constitute a large and heterogeneous group, characterized by having a dark pigment, the dihydroxynaftalen melanin-DHN, inside their cell walls. In nature they are found mainly as soil microbiota or decomposing organic matter, and are spread in tropical and subtropical regions. The fungus Fonsecaea monophora causes chromoblastomycosis in humans, and possesses essential mechanisms that may enhance pathogenicity, proliferation and dissemination inside the host. Glycoconjugates confer important properties to these pathogenic microorganisms. In this work, structural characterization of glycan structures present in two different strains of F. monophora MMHC82 and FE5p4, from clinical and environmental origins, respectively, was performed. Each one were grown on Minimal Medium (MM) and Czapeck-Dox (CD) medium, and the water soluble cell wall glycoconjugates and exopolysaccharides (EPS) were evaluated by NMR, methylation and principal component analysis (PCA). By combining the methylation and 2D NMR analyses, it was possible to visualize the glycosidic profiles of the complex carbohydrate mixtures. Significant differences were observed in β-D-Galf-(1→5) and (1→6) linkages, α- and β-D-Glcp-(1→3), (1→4), and (1→6) units, as well as in α-D-Manp. PCA from (1)H-NMR data showed that MMHC82 from CD medium showed a higher variation in the cell wall carbohydrates, mainly related to O-2 substituted β-D-Galf (δ 106.0/5.23 and δ 105.3/5.23) units. In order to investigate the antigenic response of the glycoconjugates, these were screened against serum from chromoblastomycosis patients. The antigen which contained the cell wall of MMHC82 grown in MM had β-D-Manp units that promoted higher antigenic response. The distribution of these fungal species in nature and the knowledge of how cell wall polysaccharides and glycoconjugates structure vary, may contribute to the better understanding and the elucidation of the pathology caused by this fungus.

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Individual data from immunoassays with antigens existing on the serum of patients with chromoblastomycosis (P), patients without chromoblastomycosis (N). Antigens were assessed to determine their capacity of reaction in the formation of immune complexes with wall polysaccharides of the fungus F. monophora. Antigen 1 (Ag1), belonging to the cell wall of strain MMHC82 drawn from MM, antigen 2 (Ag2), cell wall antigen of strain MMHC82 from CD medium, antigen 3 (Ag3), cell wall antigen from strain FE5p4 from MM and antigen 4 (Ag4), cell wall antigen from strain FE5p4 from CD medium.
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Figure 4: Individual data from immunoassays with antigens existing on the serum of patients with chromoblastomycosis (P), patients without chromoblastomycosis (N). Antigens were assessed to determine their capacity of reaction in the formation of immune complexes with wall polysaccharides of the fungus F. monophora. Antigen 1 (Ag1), belonging to the cell wall of strain MMHC82 drawn from MM, antigen 2 (Ag2), cell wall antigen of strain MMHC82 from CD medium, antigen 3 (Ag3), cell wall antigen from strain FE5p4 from MM and antigen 4 (Ag4), cell wall antigen from strain FE5p4 from CD medium.

Mentions: Antigens obtained from both strains grown on each condition were denominated as follows: Antigen 1 (Ag1), obtained from strain MMHC82 grown on MM; Antigen 2 (Ag2), from strain MMHC82 on CD medium; Antigen 3 (Ag3), from strain FE5p4 grown on MM; and Antigen 4 (Ag4), from strain FE5p4 on CD medium (Figure 4 and Figure S1).


Glycan analysis of Fonsecaea monophora from clinical and environmental origins reveals different structural profile and human antigenic response.

Burjack JR, Santana-Filho AP, Ruthes AC, Riter DS, Vicente VA, Alvarenga LM, Sassaki GL - Front Cell Infect Microbiol (2014)

Individual data from immunoassays with antigens existing on the serum of patients with chromoblastomycosis (P), patients without chromoblastomycosis (N). Antigens were assessed to determine their capacity of reaction in the formation of immune complexes with wall polysaccharides of the fungus F. monophora. Antigen 1 (Ag1), belonging to the cell wall of strain MMHC82 drawn from MM, antigen 2 (Ag2), cell wall antigen of strain MMHC82 from CD medium, antigen 3 (Ag3), cell wall antigen from strain FE5p4 from MM and antigen 4 (Ag4), cell wall antigen from strain FE5p4 from CD medium.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4215789&req=5

Figure 4: Individual data from immunoassays with antigens existing on the serum of patients with chromoblastomycosis (P), patients without chromoblastomycosis (N). Antigens were assessed to determine their capacity of reaction in the formation of immune complexes with wall polysaccharides of the fungus F. monophora. Antigen 1 (Ag1), belonging to the cell wall of strain MMHC82 drawn from MM, antigen 2 (Ag2), cell wall antigen of strain MMHC82 from CD medium, antigen 3 (Ag3), cell wall antigen from strain FE5p4 from MM and antigen 4 (Ag4), cell wall antigen from strain FE5p4 from CD medium.
Mentions: Antigens obtained from both strains grown on each condition were denominated as follows: Antigen 1 (Ag1), obtained from strain MMHC82 grown on MM; Antigen 2 (Ag2), from strain MMHC82 on CD medium; Antigen 3 (Ag3), from strain FE5p4 grown on MM; and Antigen 4 (Ag4), from strain FE5p4 on CD medium (Figure 4 and Figure S1).

Bottom Line: In nature they are found mainly as soil microbiota or decomposing organic matter, and are spread in tropical and subtropical regions.The antigen which contained the cell wall of MMHC82 grown in MM had β-D-Manp units that promoted higher antigenic response.The distribution of these fungal species in nature and the knowledge of how cell wall polysaccharides and glycoconjugates structure vary, may contribute to the better understanding and the elucidation of the pathology caused by this fungus.

View Article: PubMed Central - PubMed

Affiliation: Química de Carboidratos, Departamento de Patologia Básica, Universidade Federal do Paraná Curitiba, Brazil.

ABSTRACT
Dematiaceous fungi constitute a large and heterogeneous group, characterized by having a dark pigment, the dihydroxynaftalen melanin-DHN, inside their cell walls. In nature they are found mainly as soil microbiota or decomposing organic matter, and are spread in tropical and subtropical regions. The fungus Fonsecaea monophora causes chromoblastomycosis in humans, and possesses essential mechanisms that may enhance pathogenicity, proliferation and dissemination inside the host. Glycoconjugates confer important properties to these pathogenic microorganisms. In this work, structural characterization of glycan structures present in two different strains of F. monophora MMHC82 and FE5p4, from clinical and environmental origins, respectively, was performed. Each one were grown on Minimal Medium (MM) and Czapeck-Dox (CD) medium, and the water soluble cell wall glycoconjugates and exopolysaccharides (EPS) were evaluated by NMR, methylation and principal component analysis (PCA). By combining the methylation and 2D NMR analyses, it was possible to visualize the glycosidic profiles of the complex carbohydrate mixtures. Significant differences were observed in β-D-Galf-(1→5) and (1→6) linkages, α- and β-D-Glcp-(1→3), (1→4), and (1→6) units, as well as in α-D-Manp. PCA from (1)H-NMR data showed that MMHC82 from CD medium showed a higher variation in the cell wall carbohydrates, mainly related to O-2 substituted β-D-Galf (δ 106.0/5.23 and δ 105.3/5.23) units. In order to investigate the antigenic response of the glycoconjugates, these were screened against serum from chromoblastomycosis patients. The antigen which contained the cell wall of MMHC82 grown in MM had β-D-Manp units that promoted higher antigenic response. The distribution of these fungal species in nature and the knowledge of how cell wall polysaccharides and glycoconjugates structure vary, may contribute to the better understanding and the elucidation of the pathology caused by this fungus.

Show MeSH
Related in: MedlinePlus