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The cholinergic basal forebrain in the ferret and its inputs to the auditory cortex.

Bajo VM, Leach ND, Cordery PM, Nodal FR, King AJ - Eur. J. Neurosci. (2014)

Bottom Line: Epipial tracer deposits and injections of the immunotoxin ME20.4-SAP (monoclonal antibody specific for the p75 neurotrophin receptor conjugated to saporin) in the auditory cortex showed that cholinergic inputs originate almost exclusively in the ipsilateral nucleus basalis.Moreover, tracer injections in the nucleus basalis revealed a pattern of labelled fibres and terminal fields that resembled acetylcholinesterase fibre staining in the auditory cortex, with the heaviest labelling in layers II/III and in the infragranular layers.The widespread distribution of cholinergic inputs from the nucleus basalis to both primary and higher level areas of the auditory cortex suggests that acetylcholine is likely to be involved in modulating many aspects of auditory processing.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Anatomy and Genetics, University of Oxford, Sherrington Building, Parks Road, Oxford, OX1 3PT, UK.

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Examples of labelled cells in the NB after cortical tracer deposits of rhodamine in the MEG (A), fluorescein in the PEG (B) and cascade blue in the AEG (C). ChAT immunoreactivity in the NB is shown for comparison (D). Pictures were taken in the NB ipsilateral to the cortical tracer deposits (shown in Fig.6). The insets in the left bottom corner of each panel show labelled neurons at higher magnification. The orientation and calibration bars = 100 μm in D and 50 μm in the inset; these are the same in all panels and insets. ChAT, choline acetyltransferase; D, dorsal; L, lateral.
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fig07: Examples of labelled cells in the NB after cortical tracer deposits of rhodamine in the MEG (A), fluorescein in the PEG (B) and cascade blue in the AEG (C). ChAT immunoreactivity in the NB is shown for comparison (D). Pictures were taken in the NB ipsilateral to the cortical tracer deposits (shown in Fig.6). The insets in the left bottom corner of each panel show labelled neurons at higher magnification. The orientation and calibration bars = 100 μm in D and 50 μm in the inset; these are the same in all panels and insets. ChAT, choline acetyltransferase; D, dorsal; L, lateral.

Mentions: Two approaches were used to investigate projections from the BF to the auditory cortex. First, different tracers were applied to the three regions of the EG where the auditory cortex in the ferret is located in order to retrogradely label cells in the BF (n = 3, Figs6 and 7). Second, multiple injections of the immunotoxin ME20.4-SAP were made in the EG (n = 1, Figs8 and 9) to eliminate cholinergic neurons in the BF that project to the auditory cortex. Both procedures were carried out unilaterally.


The cholinergic basal forebrain in the ferret and its inputs to the auditory cortex.

Bajo VM, Leach ND, Cordery PM, Nodal FR, King AJ - Eur. J. Neurosci. (2014)

Examples of labelled cells in the NB after cortical tracer deposits of rhodamine in the MEG (A), fluorescein in the PEG (B) and cascade blue in the AEG (C). ChAT immunoreactivity in the NB is shown for comparison (D). Pictures were taken in the NB ipsilateral to the cortical tracer deposits (shown in Fig.6). The insets in the left bottom corner of each panel show labelled neurons at higher magnification. The orientation and calibration bars = 100 μm in D and 50 μm in the inset; these are the same in all panels and insets. ChAT, choline acetyltransferase; D, dorsal; L, lateral.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4215603&req=5

fig07: Examples of labelled cells in the NB after cortical tracer deposits of rhodamine in the MEG (A), fluorescein in the PEG (B) and cascade blue in the AEG (C). ChAT immunoreactivity in the NB is shown for comparison (D). Pictures were taken in the NB ipsilateral to the cortical tracer deposits (shown in Fig.6). The insets in the left bottom corner of each panel show labelled neurons at higher magnification. The orientation and calibration bars = 100 μm in D and 50 μm in the inset; these are the same in all panels and insets. ChAT, choline acetyltransferase; D, dorsal; L, lateral.
Mentions: Two approaches were used to investigate projections from the BF to the auditory cortex. First, different tracers were applied to the three regions of the EG where the auditory cortex in the ferret is located in order to retrogradely label cells in the BF (n = 3, Figs6 and 7). Second, multiple injections of the immunotoxin ME20.4-SAP were made in the EG (n = 1, Figs8 and 9) to eliminate cholinergic neurons in the BF that project to the auditory cortex. Both procedures were carried out unilaterally.

Bottom Line: Epipial tracer deposits and injections of the immunotoxin ME20.4-SAP (monoclonal antibody specific for the p75 neurotrophin receptor conjugated to saporin) in the auditory cortex showed that cholinergic inputs originate almost exclusively in the ipsilateral nucleus basalis.Moreover, tracer injections in the nucleus basalis revealed a pattern of labelled fibres and terminal fields that resembled acetylcholinesterase fibre staining in the auditory cortex, with the heaviest labelling in layers II/III and in the infragranular layers.The widespread distribution of cholinergic inputs from the nucleus basalis to both primary and higher level areas of the auditory cortex suggests that acetylcholine is likely to be involved in modulating many aspects of auditory processing.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Anatomy and Genetics, University of Oxford, Sherrington Building, Parks Road, Oxford, OX1 3PT, UK.

Show MeSH
Related in: MedlinePlus