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CCL2 release by airway smooth muscle is increased in asthma and promotes fibrocyte migration.

Singh SR, Sutcliffe A, Kaur D, Gupta S, Desai D, Saunders R, Brightling CE - Allergy (2014)

Bottom Line: Chemokine (C-C motif) ligand 2 (CCL2) levels were increased in primary ASM supernatants from asthmatics compared with healthy controls.These effects were inhibited by CCL2 neutralization.CCL2 levels were increased in blood from asthmatics compared with healthy controls, and sputum CCL2 was increased in asthmatics with bronchial wall thickening.

View Article: PubMed Central - PubMed

Affiliation: Institute for Lung Health, Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, UK.

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Related in: MedlinePlus

Chemokine (C-C motif) ligand 2 (CCL2) levels are increased in asthmatic airway smooth muscle (ASM) supernatants. (A) ASM primes migration of fibrocytes (FCs) through a G-protein-coupled receptor as demonstrated by inhibition with pertussis toxin (PTx); (B) basal chemokine levels in pooled healthy and asthmatic supernatants of ASM cells assessed by MesoScale Discovery platform (MSD); CCL2 levels in (C) healthy and asthmatic ASM cell supernatants and (D) supernatants from FCs and ASM mono- and co-cultures assessed by ELISA. Data expressed as mean ± SEM.
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fig01: Chemokine (C-C motif) ligand 2 (CCL2) levels are increased in asthmatic airway smooth muscle (ASM) supernatants. (A) ASM primes migration of fibrocytes (FCs) through a G-protein-coupled receptor as demonstrated by inhibition with pertussis toxin (PTx); (B) basal chemokine levels in pooled healthy and asthmatic supernatants of ASM cells assessed by MesoScale Discovery platform (MSD); CCL2 levels in (C) healthy and asthmatic ASM cell supernatants and (D) supernatants from FCs and ASM mono- and co-cultures assessed by ELISA. Data expressed as mean ± SEM.

Mentions: We first explored the potential role of G-protein-coupled receptors in regulating ASM-mediated FC chemokinesis. The distance of migration of adherent elongated FCs was a mean of 12.7 ± 3.2 μm from the point of origin over 4.5 h (n = 5). Airway smooth muscle significantly potentiated chemokinesis of FCs by over twofold [34.9 ± 5.7 μm, n = 6, P = 0.008, (Fig.1A)] from the point of origin over 4.5 h. PTx, an inhibitor of Gαi receptor – G-protein coupling, significantly attenuated ASM-primed FC chemokinesis by 34 ± 9%, [23.2 ± 5.0 μm from the point of origin, n = 6, P = 0.014 (Fig.1A)].


CCL2 release by airway smooth muscle is increased in asthma and promotes fibrocyte migration.

Singh SR, Sutcliffe A, Kaur D, Gupta S, Desai D, Saunders R, Brightling CE - Allergy (2014)

Chemokine (C-C motif) ligand 2 (CCL2) levels are increased in asthmatic airway smooth muscle (ASM) supernatants. (A) ASM primes migration of fibrocytes (FCs) through a G-protein-coupled receptor as demonstrated by inhibition with pertussis toxin (PTx); (B) basal chemokine levels in pooled healthy and asthmatic supernatants of ASM cells assessed by MesoScale Discovery platform (MSD); CCL2 levels in (C) healthy and asthmatic ASM cell supernatants and (D) supernatants from FCs and ASM mono- and co-cultures assessed by ELISA. Data expressed as mean ± SEM.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4215601&req=5

fig01: Chemokine (C-C motif) ligand 2 (CCL2) levels are increased in asthmatic airway smooth muscle (ASM) supernatants. (A) ASM primes migration of fibrocytes (FCs) through a G-protein-coupled receptor as demonstrated by inhibition with pertussis toxin (PTx); (B) basal chemokine levels in pooled healthy and asthmatic supernatants of ASM cells assessed by MesoScale Discovery platform (MSD); CCL2 levels in (C) healthy and asthmatic ASM cell supernatants and (D) supernatants from FCs and ASM mono- and co-cultures assessed by ELISA. Data expressed as mean ± SEM.
Mentions: We first explored the potential role of G-protein-coupled receptors in regulating ASM-mediated FC chemokinesis. The distance of migration of adherent elongated FCs was a mean of 12.7 ± 3.2 μm from the point of origin over 4.5 h (n = 5). Airway smooth muscle significantly potentiated chemokinesis of FCs by over twofold [34.9 ± 5.7 μm, n = 6, P = 0.008, (Fig.1A)] from the point of origin over 4.5 h. PTx, an inhibitor of Gαi receptor – G-protein coupling, significantly attenuated ASM-primed FC chemokinesis by 34 ± 9%, [23.2 ± 5.0 μm from the point of origin, n = 6, P = 0.014 (Fig.1A)].

Bottom Line: Chemokine (C-C motif) ligand 2 (CCL2) levels were increased in primary ASM supernatants from asthmatics compared with healthy controls.These effects were inhibited by CCL2 neutralization.CCL2 levels were increased in blood from asthmatics compared with healthy controls, and sputum CCL2 was increased in asthmatics with bronchial wall thickening.

View Article: PubMed Central - PubMed

Affiliation: Institute for Lung Health, Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, UK.

Show MeSH
Related in: MedlinePlus