CCL2 release by airway smooth muscle is increased in asthma and promotes fibrocyte migration.
Bottom Line: Chemokine (C-C motif) ligand 2 (CCL2) levels were increased in primary ASM supernatants from asthmatics compared with healthy controls.These effects were inhibited by CCL2 neutralization.CCL2 levels were increased in blood from asthmatics compared with healthy controls, and sputum CCL2 was increased in asthmatics with bronchial wall thickening.
Affiliation: Institute for Lung Health, Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, UK.Show MeSH
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Mentions: We first explored the potential role of G-protein-coupled receptors in regulating ASM-mediated FC chemokinesis. The distance of migration of adherent elongated FCs was a mean of 12.7 ± 3.2 μm from the point of origin over 4.5 h (n = 5). Airway smooth muscle significantly potentiated chemokinesis of FCs by over twofold [34.9 ± 5.7 μm, n = 6, P = 0.008, (Fig.1A)] from the point of origin over 4.5 h. PTx, an inhibitor of Gαi receptor – G-protein coupling, significantly attenuated ASM-primed FC chemokinesis by 34 ± 9%, [23.2 ± 5.0 μm from the point of origin, n = 6, P = 0.014 (Fig.1A)].
Affiliation: Institute for Lung Health, Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, UK.