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Hypersensitivity to pollen of four different species of Brassica: a clinico-immunologic evaluation in patients of respiratory allergy in India.

Singh A, Shahi S, Katiyar RK, Gaur S, Jain V - Asia Pac Allergy (2014)

Bottom Line: Protein fractions of 47, 56, 76, 87, and 90 kDa were identified as IgE binding by all the four species, however individual heterogeneity exists.Thus a local species may be more pertinent for immunotherapy.The major allergen needs to be further characterized.

View Article: PubMed Central - PubMed

Affiliation: Allergy and Aerobiology Division, Institute of Genomics & Integrative Biology, Delhi 110 037, India.

ABSTRACT

Background: Rapeseed-mustard is the second most important source of edible oil in India. Several species of Brassica are grown in different parts of country for its oilseeds.

Objective: The objective was to investigate allergenicity to antigenic extracts of pollen of 4 species of Brassica.

Methods: Brassica campestris, Brassica juncea, Brassica nigra, and Brassica napus were selected for the detailed investigation. Pollen samples from each of the four species were collected from the polliniferous materials. The antigenic and allergenic profiles of these extracts were evaluated by means of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Skin prick test, enzyme linked immuno sorbent assay and Western blot on atopic individuals.

Results: Out of the 159 atopic subjects tested, 21.38% were positive to at least one or other species of Brassica pollen, with highest skin positivity (13.20%) to B. campestris extract. Raised IgE with significant linear correlation with intensity of skin reactions was obtained. Protein fractions of 20, 25, 32, 37, 56, and 90 kDa were recognized by B. campestris and B. juncea whereas 56, 76, 87, and 90 kDa were recognized by B. nigra and B. napus as major IgE binding protein fractions. The patients also showed positivity to other inhalant pollen allergens tested.

Conclusion: IgE mediated hypersensitivity varied from 4.40% to 13.20% in Indian atopic subjects to pollen of one or the other species of Brassica. Protein fractions of 47, 56, 76, 87, and 90 kDa were identified as IgE binding by all the four species, however individual heterogeneity exists. Thus a local species may be more pertinent for immunotherapy. The major allergen needs to be further characterized.

No MeSH data available.


Related in: MedlinePlus

Immunoblot of pollen of Brassica juncea carried out with individual sera positive to it. M, molecular weight standards; P, pooled sera of positive sera; Lane 1-14, individual sera positive to pollen of B. juncea; HV, pooled sera of healthy volunteers.
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Figure 4: Immunoblot of pollen of Brassica juncea carried out with individual sera positive to it. M, molecular weight standards; P, pooled sera of positive sera; Lane 1-14, individual sera positive to pollen of B. juncea; HV, pooled sera of healthy volunteers.

Mentions: Sera of ELISA positive patients against B. juncea pollen antigen showed 10 IgE binding protein fractions as in Fig. 4. They had mol wt of 15, 20, 25, 32, 37, 47, 56, 76, 87, and 90 kDa. Protein fraction of mol wt 56, 47, and 37 kDa are of high intensity. Protein fraction of mol wt 15, 20, 25, 32, 37, 56, and 90 kDa were detected as IgE binding in more than 70% of cases (Table 4)


Hypersensitivity to pollen of four different species of Brassica: a clinico-immunologic evaluation in patients of respiratory allergy in India.

Singh A, Shahi S, Katiyar RK, Gaur S, Jain V - Asia Pac Allergy (2014)

Immunoblot of pollen of Brassica juncea carried out with individual sera positive to it. M, molecular weight standards; P, pooled sera of positive sera; Lane 1-14, individual sera positive to pollen of B. juncea; HV, pooled sera of healthy volunteers.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4215438&req=5

Figure 4: Immunoblot of pollen of Brassica juncea carried out with individual sera positive to it. M, molecular weight standards; P, pooled sera of positive sera; Lane 1-14, individual sera positive to pollen of B. juncea; HV, pooled sera of healthy volunteers.
Mentions: Sera of ELISA positive patients against B. juncea pollen antigen showed 10 IgE binding protein fractions as in Fig. 4. They had mol wt of 15, 20, 25, 32, 37, 47, 56, 76, 87, and 90 kDa. Protein fraction of mol wt 56, 47, and 37 kDa are of high intensity. Protein fraction of mol wt 15, 20, 25, 32, 37, 56, and 90 kDa were detected as IgE binding in more than 70% of cases (Table 4)

Bottom Line: Protein fractions of 47, 56, 76, 87, and 90 kDa were identified as IgE binding by all the four species, however individual heterogeneity exists.Thus a local species may be more pertinent for immunotherapy.The major allergen needs to be further characterized.

View Article: PubMed Central - PubMed

Affiliation: Allergy and Aerobiology Division, Institute of Genomics & Integrative Biology, Delhi 110 037, India.

ABSTRACT

Background: Rapeseed-mustard is the second most important source of edible oil in India. Several species of Brassica are grown in different parts of country for its oilseeds.

Objective: The objective was to investigate allergenicity to antigenic extracts of pollen of 4 species of Brassica.

Methods: Brassica campestris, Brassica juncea, Brassica nigra, and Brassica napus were selected for the detailed investigation. Pollen samples from each of the four species were collected from the polliniferous materials. The antigenic and allergenic profiles of these extracts were evaluated by means of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Skin prick test, enzyme linked immuno sorbent assay and Western blot on atopic individuals.

Results: Out of the 159 atopic subjects tested, 21.38% were positive to at least one or other species of Brassica pollen, with highest skin positivity (13.20%) to B. campestris extract. Raised IgE with significant linear correlation with intensity of skin reactions was obtained. Protein fractions of 20, 25, 32, 37, 56, and 90 kDa were recognized by B. campestris and B. juncea whereas 56, 76, 87, and 90 kDa were recognized by B. nigra and B. napus as major IgE binding protein fractions. The patients also showed positivity to other inhalant pollen allergens tested.

Conclusion: IgE mediated hypersensitivity varied from 4.40% to 13.20% in Indian atopic subjects to pollen of one or the other species of Brassica. Protein fractions of 47, 56, 76, 87, and 90 kDa were identified as IgE binding by all the four species, however individual heterogeneity exists. Thus a local species may be more pertinent for immunotherapy. The major allergen needs to be further characterized.

No MeSH data available.


Related in: MedlinePlus