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A role for human mitochondrial complex II in the production of reactive oxygen species in human skin.

Anderson A, Bowman A, Boulton SJ, Manning P, Birch-Machin MA - Redox Biol (2014)

Bottom Line: The effects within epidermal and dermal derived skin cells are compared to other tissue cell types as well as those harbouring a compromised mitochondrial status (Rho-zero A549).The results show that the complex II inhibitor, TTFA, was the only RC inhibitor to significantly increase UVA-induced ROS production in both skin cell types (P<0.05) suggesting that the role of human skin complex II in terms of influencing ROS production is more important than previously thought particularly in comparison to liver cells.Interestingly, two-fold greater maximal activity of complex II enzyme was observed in both skin cell types compared to liver (P<0.001).

View Article: PubMed Central - PubMed

Affiliation: Dermatological Sciences, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.

No MeSH data available.


Related in: MedlinePlus

Summary of relative fluorescence intensity of HaCaT cells treated with respiratory chain inhibitors for 18 h prior to exposure of 14 J/cm2 UVA. Significant increases in DHR123 fluorescence intensity over control (i.e. UVA exposure in the absence of inhibitor) were found in rotenone (Rot), antimycin (Am), TTFA and TaClo for 18 h compared to UVA alone (control) (*P<0.05, n=8, as analysed by a one-way ANOVA with Dunnett's post-hoc test). No significant difference in fluorescence intensity was found for 3NP and DPI treatment 18 h, (P>0.05). Data representative of 2 repeats, n=8 replicates for each inhibitor treatment, bars represent means±SEM.
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f0010: Summary of relative fluorescence intensity of HaCaT cells treated with respiratory chain inhibitors for 18 h prior to exposure of 14 J/cm2 UVA. Significant increases in DHR123 fluorescence intensity over control (i.e. UVA exposure in the absence of inhibitor) were found in rotenone (Rot), antimycin (Am), TTFA and TaClo for 18 h compared to UVA alone (control) (*P<0.05, n=8, as analysed by a one-way ANOVA with Dunnett's post-hoc test). No significant difference in fluorescence intensity was found for 3NP and DPI treatment 18 h, (P>0.05). Data representative of 2 repeats, n=8 replicates for each inhibitor treatment, bars represent means±SEM.


A role for human mitochondrial complex II in the production of reactive oxygen species in human skin.

Anderson A, Bowman A, Boulton SJ, Manning P, Birch-Machin MA - Redox Biol (2014)

Summary of relative fluorescence intensity of HaCaT cells treated with respiratory chain inhibitors for 18 h prior to exposure of 14 J/cm2 UVA. Significant increases in DHR123 fluorescence intensity over control (i.e. UVA exposure in the absence of inhibitor) were found in rotenone (Rot), antimycin (Am), TTFA and TaClo for 18 h compared to UVA alone (control) (*P<0.05, n=8, as analysed by a one-way ANOVA with Dunnett's post-hoc test). No significant difference in fluorescence intensity was found for 3NP and DPI treatment 18 h, (P>0.05). Data representative of 2 repeats, n=8 replicates for each inhibitor treatment, bars represent means±SEM.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4215388&req=5

f0010: Summary of relative fluorescence intensity of HaCaT cells treated with respiratory chain inhibitors for 18 h prior to exposure of 14 J/cm2 UVA. Significant increases in DHR123 fluorescence intensity over control (i.e. UVA exposure in the absence of inhibitor) were found in rotenone (Rot), antimycin (Am), TTFA and TaClo for 18 h compared to UVA alone (control) (*P<0.05, n=8, as analysed by a one-way ANOVA with Dunnett's post-hoc test). No significant difference in fluorescence intensity was found for 3NP and DPI treatment 18 h, (P>0.05). Data representative of 2 repeats, n=8 replicates for each inhibitor treatment, bars represent means±SEM.
Bottom Line: The effects within epidermal and dermal derived skin cells are compared to other tissue cell types as well as those harbouring a compromised mitochondrial status (Rho-zero A549).The results show that the complex II inhibitor, TTFA, was the only RC inhibitor to significantly increase UVA-induced ROS production in both skin cell types (P<0.05) suggesting that the role of human skin complex II in terms of influencing ROS production is more important than previously thought particularly in comparison to liver cells.Interestingly, two-fold greater maximal activity of complex II enzyme was observed in both skin cell types compared to liver (P<0.001).

View Article: PubMed Central - PubMed

Affiliation: Dermatological Sciences, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.

No MeSH data available.


Related in: MedlinePlus