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Synthesis and antibacterial activities of antibacterial peptides with a spiropyran fluorescence probe.

Chen L, Zhu Y, Yang D, Zou R, Wu J, Tian H - Sci Rep (2014)

Bottom Line: Peptides 1-3 exhibit a marked decrease in antimicrobial activity with increasing alkyl chain length.This is likely due to the Sp-Mc isomers in different polar environments forming different aggregate sizes in TBS, as demonstrated by time-dependent dynamic light scattering (DLS).Moreover, peptides 1-3 exhibited low cytotoxicity and hemolytic activity.

View Article: PubMed Central - PubMed

Affiliation: Key Lab for Advanced Materials and Institute of Fine Chemicals, East China University of Science and Technology, Shanghai 200237 (P. R. China).

ABSTRACT
In this report, antibacterial peptides 1-3 were prepared with a spiropyran fluorescence probe. The probe exhibits a change in fluorescence when isomerized from a colorless spiro-form (spiropyran, Sp) to a colored open-form (merocyanine, Mc) under different chemical environments, which can be used to study the mechanism of antimicrobial activity. Peptides 1-3 exhibit a marked decrease in antimicrobial activity with increasing alkyl chain length. This is likely due to the Sp-Mc isomers in different polar environments forming different aggregate sizes in TBS, as demonstrated by time-dependent dynamic light scattering (DLS). Moreover, peptides 1-3 exhibited low cytotoxicity and hemolytic activity. These probe-modified peptides may provide a novel approach to study the effect of structural changes on antibacterial activity, thus facilitating the design of new antimicrobial agents to combat bacterial infection.

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Related in: MedlinePlus

Antibacterial activity of peptides 1-3 toward P. aeruginosa (G−) and M. luteus (G+) on nutrient broth medium agar.
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f6: Antibacterial activity of peptides 1-3 toward P. aeruginosa (G−) and M. luteus (G+) on nutrient broth medium agar.

Mentions: The antibacterial activities of peptides 1-3 toward Gram-positive and Gram-negative bacteria on nutrient broth medium agar plates were also investigated. As shown in Figure 6, P. aeruginosa grew well in nutrient broth medium, whereas the growth of M. luteus was efficiently inhibited by peptides 1 and 2. Moreover, peptides 1 and 2 displayed higher antibacterial activity against M. luteus than did peptide 3. These results are consistent with the IC50 measurements.


Synthesis and antibacterial activities of antibacterial peptides with a spiropyran fluorescence probe.

Chen L, Zhu Y, Yang D, Zou R, Wu J, Tian H - Sci Rep (2014)

Antibacterial activity of peptides 1-3 toward P. aeruginosa (G−) and M. luteus (G+) on nutrient broth medium agar.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4215325&req=5

f6: Antibacterial activity of peptides 1-3 toward P. aeruginosa (G−) and M. luteus (G+) on nutrient broth medium agar.
Mentions: The antibacterial activities of peptides 1-3 toward Gram-positive and Gram-negative bacteria on nutrient broth medium agar plates were also investigated. As shown in Figure 6, P. aeruginosa grew well in nutrient broth medium, whereas the growth of M. luteus was efficiently inhibited by peptides 1 and 2. Moreover, peptides 1 and 2 displayed higher antibacterial activity against M. luteus than did peptide 3. These results are consistent with the IC50 measurements.

Bottom Line: Peptides 1-3 exhibit a marked decrease in antimicrobial activity with increasing alkyl chain length.This is likely due to the Sp-Mc isomers in different polar environments forming different aggregate sizes in TBS, as demonstrated by time-dependent dynamic light scattering (DLS).Moreover, peptides 1-3 exhibited low cytotoxicity and hemolytic activity.

View Article: PubMed Central - PubMed

Affiliation: Key Lab for Advanced Materials and Institute of Fine Chemicals, East China University of Science and Technology, Shanghai 200237 (P. R. China).

ABSTRACT
In this report, antibacterial peptides 1-3 were prepared with a spiropyran fluorescence probe. The probe exhibits a change in fluorescence when isomerized from a colorless spiro-form (spiropyran, Sp) to a colored open-form (merocyanine, Mc) under different chemical environments, which can be used to study the mechanism of antimicrobial activity. Peptides 1-3 exhibit a marked decrease in antimicrobial activity with increasing alkyl chain length. This is likely due to the Sp-Mc isomers in different polar environments forming different aggregate sizes in TBS, as demonstrated by time-dependent dynamic light scattering (DLS). Moreover, peptides 1-3 exhibited low cytotoxicity and hemolytic activity. These probe-modified peptides may provide a novel approach to study the effect of structural changes on antibacterial activity, thus facilitating the design of new antimicrobial agents to combat bacterial infection.

Show MeSH
Related in: MedlinePlus