Incorporation of pentraxin 3 into hyaluronan matrices is tightly regulated and promotes matrix cross-linking.
Bottom Line: We found that PTX3 binds neither to HA alone nor to HA films containing TSG-6.Interestingly, prior encounter with IαI was required for effective incorporation of PTX3 into TSG-6-loaded HA films.We propose that this mechanism is essential for correct assembly of the COC matrix and may also have general implications in other inflammatory processes that are associated with HA cross-linking.
Affiliation: From the CIC biomaGUNE, 20009 Donostia-San Sebastian, Spain.Show MeSH
Mentions: Salustri et al. (12) hypothesized that TSG-6 mediates the incorporation of PTX3 into HA matrices. More recently, surface plasmon resonance assays have demonstrated that the binding of TSG-6 to PTX3 is mediated by its Link module and that the binding sites for PTX3 and HA are different (60). Our data on incorporation of PTX3 into HA films via Link_TSG6 are consistent with these findings (Figs. 2A and 3A; illustrated in Fig. 11A).
Affiliation: From the CIC biomaGUNE, 20009 Donostia-San Sebastian, Spain.