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Porcine SLITRK1: Molecular cloning and characterization.

Larsen K, Momeni J, Farajzadeh L, Bendixen C - FEBS Open Bio (2014)

Bottom Line: RT-PCR cloning revealed two SLITRK1 transcripts: a full-length mRNA and a transcript variant that results in a truncated protein.The encoded SLITRK1 protein, consisting of 695 amino acids, displays a very high homology to human SLITRK1 (99%).The porcine SLITRK1 gene is expressed exclusively in brain tissues.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology and Genetics, Aarhus University, Blichers Alle 20, DK-8830 Tjele, Denmark.

ABSTRACT
The membrane protein SLITRK1 functions as a developmentally regulated stimulator of neurite outgrowth and variants in this gene have been implicated in Tourette syndrome. In the current study we have cloned and characterized the porcine SLITRK1 gene. The genomic organization of SLITRK1 lacks introns, as does its human and mouse counterparts. RT-PCR cloning revealed two SLITRK1 transcripts: a full-length mRNA and a transcript variant that results in a truncated protein. The encoded SLITRK1 protein, consisting of 695 amino acids, displays a very high homology to human SLITRK1 (99%). The porcine SLITRK1 gene is expressed exclusively in brain tissues.

No MeSH data available.


Related in: MedlinePlus

Splice variant of porcine SLITRK1. Amino acid sequence alignment of porcine SLITRK1 (wt) and a splice variant, SLITRK1-Sp1, hereof. (A). The SLITRK1-Sp1 encodes a truncated version of the full length protein.
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f0015: Splice variant of porcine SLITRK1. Amino acid sequence alignment of porcine SLITRK1 (wt) and a splice variant, SLITRK1-Sp1, hereof. (A). The SLITRK1-Sp1 encodes a truncated version of the full length protein.

Mentions: Both transcripts amplified were approx. 2100 bp of size. One transcript, designated SsSLITRK1-wt in Fig. 3, displayed 100% nucleotide identity with the SLITRK1 gene. The SsSLITRK1-wt clone consisted of a coding region of 2088 nucleotides and six nucleotides of 3′UTR sequence. In addition to the full-length SLITRK1 cDNA a variant encoding a truncated SLITRK1 polypeptide was found, SsSLITRK1-tv1 (Fig. 3). The first 44 amino acids of SsSLITRK1-tv1 are completely identical with the wt amino acids sequence. Deletion of an adenosine at position 133 in the genomic SLITRLK1 sequence (GenBank ID: KJ210858), i.e. at the triplet encoding Lys45, results in a change in reading frame creating a truncated protein. The predicted amino acid sequence of the mutated SLITRK1 shows 61 nonsynonymous substitutions followed by a premature stop codon (TGA). The truncated porcine SLITRK1 protein lacks a substantial portion of the full-length protein, 651 out of 695 amino acids, including the two LRR repeats, LRRCT, LRRNT and the transmembrane region (Fig. 1). The truncated SLITRK1 protein has an estimated molecular mass of 12.1 kDa and a pI of 5.2. The carboxy terminal end of the truncated SLITRK1 protein of 107 amino acids shows a very low homology (around 40%) to cytochrome b-c1 complex subunit from horse. The truncated SLITRK1 may therefore very likely play a different role than the mature protein. However, there is presently no evidence for a different function.


Porcine SLITRK1: Molecular cloning and characterization.

Larsen K, Momeni J, Farajzadeh L, Bendixen C - FEBS Open Bio (2014)

Splice variant of porcine SLITRK1. Amino acid sequence alignment of porcine SLITRK1 (wt) and a splice variant, SLITRK1-Sp1, hereof. (A). The SLITRK1-Sp1 encodes a truncated version of the full length protein.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4215120&req=5

f0015: Splice variant of porcine SLITRK1. Amino acid sequence alignment of porcine SLITRK1 (wt) and a splice variant, SLITRK1-Sp1, hereof. (A). The SLITRK1-Sp1 encodes a truncated version of the full length protein.
Mentions: Both transcripts amplified were approx. 2100 bp of size. One transcript, designated SsSLITRK1-wt in Fig. 3, displayed 100% nucleotide identity with the SLITRK1 gene. The SsSLITRK1-wt clone consisted of a coding region of 2088 nucleotides and six nucleotides of 3′UTR sequence. In addition to the full-length SLITRK1 cDNA a variant encoding a truncated SLITRK1 polypeptide was found, SsSLITRK1-tv1 (Fig. 3). The first 44 amino acids of SsSLITRK1-tv1 are completely identical with the wt amino acids sequence. Deletion of an adenosine at position 133 in the genomic SLITRLK1 sequence (GenBank ID: KJ210858), i.e. at the triplet encoding Lys45, results in a change in reading frame creating a truncated protein. The predicted amino acid sequence of the mutated SLITRK1 shows 61 nonsynonymous substitutions followed by a premature stop codon (TGA). The truncated porcine SLITRK1 protein lacks a substantial portion of the full-length protein, 651 out of 695 amino acids, including the two LRR repeats, LRRCT, LRRNT and the transmembrane region (Fig. 1). The truncated SLITRK1 protein has an estimated molecular mass of 12.1 kDa and a pI of 5.2. The carboxy terminal end of the truncated SLITRK1 protein of 107 amino acids shows a very low homology (around 40%) to cytochrome b-c1 complex subunit from horse. The truncated SLITRK1 may therefore very likely play a different role than the mature protein. However, there is presently no evidence for a different function.

Bottom Line: RT-PCR cloning revealed two SLITRK1 transcripts: a full-length mRNA and a transcript variant that results in a truncated protein.The encoded SLITRK1 protein, consisting of 695 amino acids, displays a very high homology to human SLITRK1 (99%).The porcine SLITRK1 gene is expressed exclusively in brain tissues.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology and Genetics, Aarhus University, Blichers Alle 20, DK-8830 Tjele, Denmark.

ABSTRACT
The membrane protein SLITRK1 functions as a developmentally regulated stimulator of neurite outgrowth and variants in this gene have been implicated in Tourette syndrome. In the current study we have cloned and characterized the porcine SLITRK1 gene. The genomic organization of SLITRK1 lacks introns, as does its human and mouse counterparts. RT-PCR cloning revealed two SLITRK1 transcripts: a full-length mRNA and a transcript variant that results in a truncated protein. The encoded SLITRK1 protein, consisting of 695 amino acids, displays a very high homology to human SLITRK1 (99%). The porcine SLITRK1 gene is expressed exclusively in brain tissues.

No MeSH data available.


Related in: MedlinePlus