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Near-infrared low-level laser stimulation of telocytes from human myometrium.

Campeanu RA, Radu BM, Cretoiu SM, Banciu DD, Banciu A, Cretoiu D, Popescu LM - Lasers Med Sci (2014)

Bottom Line: Acute exposure (30 min) of TCs from pregnant myometrium to 1 μM mibefradil, a selective inhibitor of T-type calcium channels, determines a significant reduction in the LLLS TLE growth rate (5.7 ± 0.8 μm/min) compared to LLLS per se in same type of samples.In conclusion, TCs from pregnant myometrium are more susceptible of reacting to LLLS than those from nonpregnant myometrium.Therefore, some implications are emerging for low-level laser therapy (LLLT) in uterine regenerative medicine.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy Animal Physiology and Biophysics, Faculty of Biology, University of Bucharest, 050095, Bucharest, Romania.

ABSTRACT
Telocytes (TCs) are a brand-new cell type frequently observed in the interstitial space of many organs (see www.telocytes.com ). TCs are defined by very long (tens of micrometers) and slender prolongations named telopodes. At their level, dilations-called podoms (~300 nm), alternate with podomers (80-100 nm). TCs were identified in a myometrial interstitial cell culture based on morphological criteria and by CD34 and PDGF receptor alpha (PDGFRα) immunopositivity. However, the mechanism(s) of telopodes formation and/or elongation and ramification is not known. We report here the low-level laser stimulation (LLLS) using a 1,064-nm neodymium-doped yttrium aluminum garnet (Nd:YAG) laser (with an output power of 60 mW) of the telopodal lateral extension (TLE) growth in cell culture. LLLS of TCs determines a higher growth rate of TLE in pregnant myometrium primary cultures (10.3 ± 1.0 μm/min) compared to nonpregnant ones (6.6 ± 0.9 μm/min). Acute exposure (30 min) of TCs from pregnant myometrium to 1 μM mibefradil, a selective inhibitor of T-type calcium channels, determines a significant reduction in the LLLS TLE growth rate (5.7 ± 0.8 μm/min) compared to LLLS per se in same type of samples. Meanwhile, chronic exposure (24 h) completely abolishes the LLLS TLE growth in both nonpregnant and pregnant myometria. The initial direction of TLE growth was modified by LLLS, the angle of deviation being more accentuated in TCs from human pregnant myometrium than in TCs from nonpregnant myometrium. In conclusion, TCs from pregnant myometrium are more susceptible of reacting to LLLS than those from nonpregnant myometrium. Therefore, some implications are emerging for low-level laser therapy (LLLT) in uterine regenerative medicine.

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The comparative average growth rate of TLE upon LLLS between TCs from pregnant myometrium before and after acute mibefradil (30 min) treatment. *p < 0.05, paired Student’s t test
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Fig5: The comparative average growth rate of TLE upon LLLS between TCs from pregnant myometrium before and after acute mibefradil (30 min) treatment. *p < 0.05, paired Student’s t test

Mentions: The chronic exposure to mibefradil determined a decrease in the growth rate, 5.7 ± 0.8 μm/min (n = 3), that is significantly lower than that in control conditions (9.7 ± 0.4 μm/min, n = 3; p < 0.05, paired Student’s t test; Fig. 5). The control value of the growth rate for pregnant myometrium was found to be different from the above-reported values. It should be noted that the LLLS growth rate after acute mibefradil treatment in pregnant myometrium is below the growth rate for control nonpregnant myometrium (p < 0.05, unpaired Student’s t test). After chronic exposure to mibefradil, LLLS performed on TCs from pregnant myometrium indicated an inhibition of the growth process.Fig. 5


Near-infrared low-level laser stimulation of telocytes from human myometrium.

Campeanu RA, Radu BM, Cretoiu SM, Banciu DD, Banciu A, Cretoiu D, Popescu LM - Lasers Med Sci (2014)

The comparative average growth rate of TLE upon LLLS between TCs from pregnant myometrium before and after acute mibefradil (30 min) treatment. *p < 0.05, paired Student’s t test
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4215113&req=5

Fig5: The comparative average growth rate of TLE upon LLLS between TCs from pregnant myometrium before and after acute mibefradil (30 min) treatment. *p < 0.05, paired Student’s t test
Mentions: The chronic exposure to mibefradil determined a decrease in the growth rate, 5.7 ± 0.8 μm/min (n = 3), that is significantly lower than that in control conditions (9.7 ± 0.4 μm/min, n = 3; p < 0.05, paired Student’s t test; Fig. 5). The control value of the growth rate for pregnant myometrium was found to be different from the above-reported values. It should be noted that the LLLS growth rate after acute mibefradil treatment in pregnant myometrium is below the growth rate for control nonpregnant myometrium (p < 0.05, unpaired Student’s t test). After chronic exposure to mibefradil, LLLS performed on TCs from pregnant myometrium indicated an inhibition of the growth process.Fig. 5

Bottom Line: Acute exposure (30 min) of TCs from pregnant myometrium to 1 μM mibefradil, a selective inhibitor of T-type calcium channels, determines a significant reduction in the LLLS TLE growth rate (5.7 ± 0.8 μm/min) compared to LLLS per se in same type of samples.In conclusion, TCs from pregnant myometrium are more susceptible of reacting to LLLS than those from nonpregnant myometrium.Therefore, some implications are emerging for low-level laser therapy (LLLT) in uterine regenerative medicine.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy Animal Physiology and Biophysics, Faculty of Biology, University of Bucharest, 050095, Bucharest, Romania.

ABSTRACT
Telocytes (TCs) are a brand-new cell type frequently observed in the interstitial space of many organs (see www.telocytes.com ). TCs are defined by very long (tens of micrometers) and slender prolongations named telopodes. At their level, dilations-called podoms (~300 nm), alternate with podomers (80-100 nm). TCs were identified in a myometrial interstitial cell culture based on morphological criteria and by CD34 and PDGF receptor alpha (PDGFRα) immunopositivity. However, the mechanism(s) of telopodes formation and/or elongation and ramification is not known. We report here the low-level laser stimulation (LLLS) using a 1,064-nm neodymium-doped yttrium aluminum garnet (Nd:YAG) laser (with an output power of 60 mW) of the telopodal lateral extension (TLE) growth in cell culture. LLLS of TCs determines a higher growth rate of TLE in pregnant myometrium primary cultures (10.3 ± 1.0 μm/min) compared to nonpregnant ones (6.6 ± 0.9 μm/min). Acute exposure (30 min) of TCs from pregnant myometrium to 1 μM mibefradil, a selective inhibitor of T-type calcium channels, determines a significant reduction in the LLLS TLE growth rate (5.7 ± 0.8 μm/min) compared to LLLS per se in same type of samples. Meanwhile, chronic exposure (24 h) completely abolishes the LLLS TLE growth in both nonpregnant and pregnant myometria. The initial direction of TLE growth was modified by LLLS, the angle of deviation being more accentuated in TCs from human pregnant myometrium than in TCs from nonpregnant myometrium. In conclusion, TCs from pregnant myometrium are more susceptible of reacting to LLLS than those from nonpregnant myometrium. Therefore, some implications are emerging for low-level laser therapy (LLLT) in uterine regenerative medicine.

Show MeSH
Related in: MedlinePlus