Human IgE against the major allergen Bet v 1--defining an epitope with limited cross-reactivity between different PR-10 family proteins.
Bottom Line: This provides a potential explanation, at the molecular level, for the differences in recognition of isoforms of Bet v 1 and other allergens in the PR-10 protein family displayed by IgE targeting this epitope.Finally, we present the first high-resolution structure of a human allergen-specific IgE fragment in the single-chain fragment variable (scFv) format.Such studies may aid us in development of better diagnostic tools and guide us in the development of new therapeutic compounds.
Affiliation: Department of Immunotechnology, Lund University, Lund, Sweden.Show MeSH
Related in: MedlinePlus
Mentions: The crystal structure of the M0418 scFv was determined to 1.3Å resolution. The structures of the two independent molecules of the asymmetric unit were similar and were superposed with an RMSD value of 0.56Å over 232 Cα atoms. The most significant difference between the two molecules was disorder of a stretch of residues from CDRH3 of molecule B (residues 110-112.3), while that from molecule A was completely modelled. In molecule A, CDRH3 extends ˜8Å above the surface of the scFv (Fig.5a).
Affiliation: Department of Immunotechnology, Lund University, Lund, Sweden.