Human IgE against the major allergen Bet v 1--defining an epitope with limited cross-reactivity between different PR-10 family proteins.
Bottom Line: The interaction between IgE and allergen is a key event at the initiation of an allergic response, and its characteristics have substantial effects on the clinical manifestation.We here display the usefulness of allergen-specific human monoclonal IgE as a tool in studies of the crucial molecular interaction taking place at the initiation of an allergic response.Such studies may aid us in development of better diagnostic tools and guide us in the development of new therapeutic compounds.
Affiliation: Department of Immunotechnology, Lund University, Lund, Sweden.Show MeSH
Mentions: To map the epitopes of the available scFv-CHε2-4 fusion proteins, their ability to bind to a set of peptides covering the amino acid sequence of Bet v 1.0101 was assessed by ELISA (Figure S4). Clone M0418 showed strong and B14 showed weak reactivity to a peptide covering residues I56 to D69, while B13 bound weakly to a peptide covering residues G26 to S39. B10 failed to bind any of the tested peptides, but, as mentioned above, should likely bind the same epitope as B13. These binding specificities were confirmed by testing the ability of the identified peptides in soluble form to inhibit binding to immobilized Bet v 1 (Fig.4a).
Affiliation: Department of Immunotechnology, Lund University, Lund, Sweden.