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Prophylactic and therapeutic vaccination with carrier-bound Bet v 1 peptides lacking allergen-specific T cell epitopes reduces Bet v 1-specific T cell responses via blocking antibodies in a murine model for birch pollen allergy.

Linhart B, Narayanan M, Focke-Tejkl M, Wrba F, Vrtala S, Valenta R - Clin. Exp. Allergy (2014)

Bottom Line: The effects of peptide-specific and allergen-specific antibodies on T cell responses and allergic lung inflammation were studied using specific antibodies.Prophylactic and therapeutic vaccination of mice with the peptide vaccine induced Bet v 1-specific antibodies which suppressed Bet v 1-specific T cell responses and allergic lung inflammation.Vaccination with carrier-bound allergen-derived peptides lacking allergen-specific T cell epitopes induces allergen-specific IgG antibodies which suppress allergen-specific T cell responses and allergic lung inflammation.

View Article: PubMed Central - PubMed

Affiliation: Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.

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Adminstration of Bet v 1 peptide-specific antibodies prevents allergic lung inflammation. (a) Two groups of mice (n = 5) were sensitized with Bet v 1 and one group received PBS. One group of sensitized mice received Bet v 1 peptide-specific antibodies or Phl p 1-specific antibodies, and lung inflammation was induced by intranasal application of Bet v 1. (b) Mean OD values ± SD corresponding to Bet v 1-specific IgE levels (y-axis) are shown for the three mouse groups at different time points (x-axis). (c) Presence of Bet v 1-specific rabbit IgG antibodies in sera of the three groups of mice as in (b). H&E-stained lung sections from a representative mouse from the PBS group (d), the group treated with Bet v 1-peptide-specific antibodies (e), or with Phl p 1-specific antibodies (f). Percentages of infiltrated areas are shown in each section (d–f).
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fig08: Adminstration of Bet v 1 peptide-specific antibodies prevents allergic lung inflammation. (a) Two groups of mice (n = 5) were sensitized with Bet v 1 and one group received PBS. One group of sensitized mice received Bet v 1 peptide-specific antibodies or Phl p 1-specific antibodies, and lung inflammation was induced by intranasal application of Bet v 1. (b) Mean OD values ± SD corresponding to Bet v 1-specific IgE levels (y-axis) are shown for the three mouse groups at different time points (x-axis). (c) Presence of Bet v 1-specific rabbit IgG antibodies in sera of the three groups of mice as in (b). H&E-stained lung sections from a representative mouse from the PBS group (d), the group treated with Bet v 1-peptide-specific antibodies (e), or with Phl p 1-specific antibodies (f). Percentages of infiltrated areas are shown in each section (d–f).

Mentions: Groups of mice (n = 5) were sensitized to Bet v 1 by one injection of alum-adsorbed Bet v 1 (10 μg). On day 28, rabbit anti-Bet v 1 peptide 2, 3 and 4 antisera or an irrelevant rabbit antiserum (anti-Phl p 1) were applied i.p. On three consecutive days, 5 μg Bet v 1 per mouse in 50 μL PBS was given intranasally to anesthetized mice. After killing, lung tissue was analysed for lymphocyte infiltration (Fig.8). Lung sections of 4 μm were stained with haematoxylin and eosin (H&E). The three most densely infiltrated areas in each H&E section were evaluated using an ocular grid with 100 small squares, each single square area 0.01 mm2. Percentages of inflammation area were calculated by counting the cells in the infiltrates within 3 single squares expressed as mean percentages.


Prophylactic and therapeutic vaccination with carrier-bound Bet v 1 peptides lacking allergen-specific T cell epitopes reduces Bet v 1-specific T cell responses via blocking antibodies in a murine model for birch pollen allergy.

Linhart B, Narayanan M, Focke-Tejkl M, Wrba F, Vrtala S, Valenta R - Clin. Exp. Allergy (2014)

Adminstration of Bet v 1 peptide-specific antibodies prevents allergic lung inflammation. (a) Two groups of mice (n = 5) were sensitized with Bet v 1 and one group received PBS. One group of sensitized mice received Bet v 1 peptide-specific antibodies or Phl p 1-specific antibodies, and lung inflammation was induced by intranasal application of Bet v 1. (b) Mean OD values ± SD corresponding to Bet v 1-specific IgE levels (y-axis) are shown for the three mouse groups at different time points (x-axis). (c) Presence of Bet v 1-specific rabbit IgG antibodies in sera of the three groups of mice as in (b). H&E-stained lung sections from a representative mouse from the PBS group (d), the group treated with Bet v 1-peptide-specific antibodies (e), or with Phl p 1-specific antibodies (f). Percentages of infiltrated areas are shown in each section (d–f).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4215111&req=5

fig08: Adminstration of Bet v 1 peptide-specific antibodies prevents allergic lung inflammation. (a) Two groups of mice (n = 5) were sensitized with Bet v 1 and one group received PBS. One group of sensitized mice received Bet v 1 peptide-specific antibodies or Phl p 1-specific antibodies, and lung inflammation was induced by intranasal application of Bet v 1. (b) Mean OD values ± SD corresponding to Bet v 1-specific IgE levels (y-axis) are shown for the three mouse groups at different time points (x-axis). (c) Presence of Bet v 1-specific rabbit IgG antibodies in sera of the three groups of mice as in (b). H&E-stained lung sections from a representative mouse from the PBS group (d), the group treated with Bet v 1-peptide-specific antibodies (e), or with Phl p 1-specific antibodies (f). Percentages of infiltrated areas are shown in each section (d–f).
Mentions: Groups of mice (n = 5) were sensitized to Bet v 1 by one injection of alum-adsorbed Bet v 1 (10 μg). On day 28, rabbit anti-Bet v 1 peptide 2, 3 and 4 antisera or an irrelevant rabbit antiserum (anti-Phl p 1) were applied i.p. On three consecutive days, 5 μg Bet v 1 per mouse in 50 μL PBS was given intranasally to anesthetized mice. After killing, lung tissue was analysed for lymphocyte infiltration (Fig.8). Lung sections of 4 μm were stained with haematoxylin and eosin (H&E). The three most densely infiltrated areas in each H&E section were evaluated using an ocular grid with 100 small squares, each single square area 0.01 mm2. Percentages of inflammation area were calculated by counting the cells in the infiltrates within 3 single squares expressed as mean percentages.

Bottom Line: The effects of peptide-specific and allergen-specific antibodies on T cell responses and allergic lung inflammation were studied using specific antibodies.Prophylactic and therapeutic vaccination of mice with the peptide vaccine induced Bet v 1-specific antibodies which suppressed Bet v 1-specific T cell responses and allergic lung inflammation.Vaccination with carrier-bound allergen-derived peptides lacking allergen-specific T cell epitopes induces allergen-specific IgG antibodies which suppress allergen-specific T cell responses and allergic lung inflammation.

View Article: PubMed Central - PubMed

Affiliation: Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.

Show MeSH