Prophylactic and therapeutic vaccination with carrier-bound Bet v 1 peptides lacking allergen-specific T cell epitopes reduces Bet v 1-specific T cell responses via blocking antibodies in a murine model for birch pollen allergy.
Bottom Line: The effects of peptide-specific and allergen-specific antibodies on T cell responses and allergic lung inflammation were studied using specific antibodies.Prophylactic and therapeutic vaccination of mice with the peptide vaccine induced Bet v 1-specific antibodies which suppressed Bet v 1-specific T cell responses and allergic lung inflammation.Vaccination with carrier-bound allergen-derived peptides lacking allergen-specific T cell epitopes induces allergen-specific IgG antibodies which suppress allergen-specific T cell responses and allergic lung inflammation.
Affiliation: Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.Show MeSH
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Mentions: Groups of mice (n = 5) were sensitized to Bet v 1 by one injection of alum-adsorbed Bet v 1 (10 μg). On day 28, rabbit anti-Bet v 1 peptide 2, 3 and 4 antisera or an irrelevant rabbit antiserum (anti-Phl p 1) were applied i.p. On three consecutive days, 5 μg Bet v 1 per mouse in 50 μL PBS was given intranasally to anesthetized mice. After killing, lung tissue was analysed for lymphocyte infiltration (Fig.8). Lung sections of 4 μm were stained with haematoxylin and eosin (H&E). The three most densely infiltrated areas in each H&E section were evaluated using an ocular grid with 100 small squares, each single square area 0.01 mm2. Percentages of inflammation area were calculated by counting the cells in the infiltrates within 3 single squares expressed as mean percentages.
Affiliation: Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.