Prophylactic and therapeutic vaccination with carrier-bound Bet v 1 peptides lacking allergen-specific T cell epitopes reduces Bet v 1-specific T cell responses via blocking antibodies in a murine model for birch pollen allergy.
Bottom Line: The effects of peptide-specific and allergen-specific antibodies on T cell responses and allergic lung inflammation were studied using specific antibodies.Prophylactic and therapeutic vaccination of mice with the peptide vaccine induced Bet v 1-specific antibodies which suppressed Bet v 1-specific T cell responses and allergic lung inflammation.Vaccination with carrier-bound allergen-derived peptides lacking allergen-specific T cell epitopes induces allergen-specific IgG antibodies which suppress allergen-specific T cell responses and allergic lung inflammation.
Affiliation: Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.Show MeSH
Mentions: Next, we investigated the influence of peptide vaccination on the development of allergen-specific T cell responses in the course of allergic sensitization. Compared with the unvaccinated but sensitized group (P−/S+), which developed a strong Bet v 1-specific T cell response with stimulation indices above 20 (Fig.5a, P−/S+), there was only low proliferation in response to the Bet v 1 allergen detectable in the mouse group which had received prophylactic peptide vaccination (Fig.5a, P+/S+). The latter response was significantly lower than that in mice without prophylactic vaccination (P < 0.05). No Bet v 1-specific T cell responses were observed in mice who had only received PBS or in mice who were vaccinated with the peptide vaccine but were not sensitized (P+/S−) (Fig.5a). None of the mouse groups showed any relevant proliferative responses to a mix of the three uncoupled Bet v 1 peptides, pep 2, 3, and 4 (Fig.5b). Mice that had received peptide vaccination but not unvaccinated or sensitized mice showed specific splenocyte proliferative responses to the carrier protein KLH (Fig.5c, P+/S−, P+/S+).
Affiliation: Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.