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High proportions of FOXP3(+) CD25(high) T cells in neonates are positively associated with allergic sensitization later in childhood.

Strömbeck A, Rabe H, Lundell AC, Andersson K, Johansen S, Adlerberth I, Wold AE, Hesselmar B, Rudin A - Clin. Exp. Allergy (2014)

Bottom Line: A clinical evaluation regarding the presence of allergen-specific IgE and allergy was performed at 18 and 36 months of age.Multivariate discriminant analysis revealed that children who were sensitized at 18 or 36 months of age had higher proportions of FOXP3(+) CD25(high) T cells at birth and at 3 days of life than children who remained non-sensitized, whereas allergy was unrelated to the neonatal proportions of these cells.Finally, a farming environment was associated with lower proportions of FOXP3(+) CD25(high) T cells in early infancy and to a more prominent T cell memory conversion and cytokine production.

View Article: PubMed Central - PubMed

Affiliation: Department of Rheumatology and Inflammation Research, The Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden.

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(a and b) The proportions of FOXP3+CD25high cells within theCD4+ T cell population at birth and at 3–5 days in children who weresensitized, that is, specific IgE to food and/or inhalant allergens, or non-sensitized at (a) 18 or(b) 36 months of age. (c and d) The proportions ofCTLA-4+CD25+ cells within the CD4+ T cellpopulation at birth and at 3–5 days in children who were sensitized or non-sensitizedat (c) 18 or (d) 36 months of age. Each dot represents an individual, and horizontal barsindicate median value. Statistical differences between the groups were calculated using two-tailedMann–Whitney U-test.P ≤ 0.05 was regarded as significant(*P ≤ 0.05; **P ≤ 0.01; and ***P ≤ 0.001). (e) Gating strategies for FOXP3or CTLA-4 expression within the various CD25+ T cell populations in cord blood.Numbers represent the percentage of cells within the gate.
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fig02: (a and b) The proportions of FOXP3+CD25high cells within theCD4+ T cell population at birth and at 3–5 days in children who weresensitized, that is, specific IgE to food and/or inhalant allergens, or non-sensitized at (a) 18 or(b) 36 months of age. (c and d) The proportions ofCTLA-4+CD25+ cells within the CD4+ T cellpopulation at birth and at 3–5 days in children who were sensitized or non-sensitizedat (c) 18 or (d) 36 months of age. Each dot represents an individual, and horizontal barsindicate median value. Statistical differences between the groups were calculated using two-tailedMann–Whitney U-test.P ≤ 0.05 was regarded as significant(*P ≤ 0.05; **P ≤ 0.01; and ***P ≤ 0.001). (e) Gating strategies for FOXP3or CTLA-4 expression within the various CD25+ T cell populations in cord blood.Numbers represent the percentage of cells within the gate.

Mentions: Univariate analyses demonstrate that children who were sensitized at 18 or 36 months ofage had significantly higher proportions of FOXP3+CD25high cells withinthe CD4+ T cell population at birth and at 3 days of life thannon-sensitized children (Figs2a and b). Despite the factthat Tregs are considered to express the immunoregulatory molecule CTLA-4 25,26, the proportions ofCTLA-4+CD25+ T cells were unrelated to sensitization in bothmultivariate (Figs1c and d) and univariate analyses(Figs2c and d). The gating strategies forFOXP3+CD25high and CTLA-4+CD25+expression within the CD4+ T cell population are demonstrated in Fig.2e.


High proportions of FOXP3(+) CD25(high) T cells in neonates are positively associated with allergic sensitization later in childhood.

Strömbeck A, Rabe H, Lundell AC, Andersson K, Johansen S, Adlerberth I, Wold AE, Hesselmar B, Rudin A - Clin. Exp. Allergy (2014)

(a and b) The proportions of FOXP3+CD25high cells within theCD4+ T cell population at birth and at 3–5 days in children who weresensitized, that is, specific IgE to food and/or inhalant allergens, or non-sensitized at (a) 18 or(b) 36 months of age. (c and d) The proportions ofCTLA-4+CD25+ cells within the CD4+ T cellpopulation at birth and at 3–5 days in children who were sensitized or non-sensitizedat (c) 18 or (d) 36 months of age. Each dot represents an individual, and horizontal barsindicate median value. Statistical differences between the groups were calculated using two-tailedMann–Whitney U-test.P ≤ 0.05 was regarded as significant(*P ≤ 0.05; **P ≤ 0.01; and ***P ≤ 0.001). (e) Gating strategies for FOXP3or CTLA-4 expression within the various CD25+ T cell populations in cord blood.Numbers represent the percentage of cells within the gate.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4215110&req=5

fig02: (a and b) The proportions of FOXP3+CD25high cells within theCD4+ T cell population at birth and at 3–5 days in children who weresensitized, that is, specific IgE to food and/or inhalant allergens, or non-sensitized at (a) 18 or(b) 36 months of age. (c and d) The proportions ofCTLA-4+CD25+ cells within the CD4+ T cellpopulation at birth and at 3–5 days in children who were sensitized or non-sensitizedat (c) 18 or (d) 36 months of age. Each dot represents an individual, and horizontal barsindicate median value. Statistical differences between the groups were calculated using two-tailedMann–Whitney U-test.P ≤ 0.05 was regarded as significant(*P ≤ 0.05; **P ≤ 0.01; and ***P ≤ 0.001). (e) Gating strategies for FOXP3or CTLA-4 expression within the various CD25+ T cell populations in cord blood.Numbers represent the percentage of cells within the gate.
Mentions: Univariate analyses demonstrate that children who were sensitized at 18 or 36 months ofage had significantly higher proportions of FOXP3+CD25high cells withinthe CD4+ T cell population at birth and at 3 days of life thannon-sensitized children (Figs2a and b). Despite the factthat Tregs are considered to express the immunoregulatory molecule CTLA-4 25,26, the proportions ofCTLA-4+CD25+ T cells were unrelated to sensitization in bothmultivariate (Figs1c and d) and univariate analyses(Figs2c and d). The gating strategies forFOXP3+CD25high and CTLA-4+CD25+expression within the CD4+ T cell population are demonstrated in Fig.2e.

Bottom Line: A clinical evaluation regarding the presence of allergen-specific IgE and allergy was performed at 18 and 36 months of age.Multivariate discriminant analysis revealed that children who were sensitized at 18 or 36 months of age had higher proportions of FOXP3(+) CD25(high) T cells at birth and at 3 days of life than children who remained non-sensitized, whereas allergy was unrelated to the neonatal proportions of these cells.Finally, a farming environment was associated with lower proportions of FOXP3(+) CD25(high) T cells in early infancy and to a more prominent T cell memory conversion and cytokine production.

View Article: PubMed Central - PubMed

Affiliation: Department of Rheumatology and Inflammation Research, The Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden.

Show MeSH
Related in: MedlinePlus