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High proportions of FOXP3(+) CD25(high) T cells in neonates are positively associated with allergic sensitization later in childhood.

Strömbeck A, Rabe H, Lundell AC, Andersson K, Johansen S, Adlerberth I, Wold AE, Hesselmar B, Rudin A - Clin. Exp. Allergy (2014)

Bottom Line: The association between higher proportions of FOXP3(+) CD25(high) T cells and sensitization persisted after exclusion of farmer's children.Finally, a farming environment was associated with lower proportions of FOXP3(+) CD25(high) T cells in early infancy and to a more prominent T cell memory conversion and cytokine production.Our results indicate that high proportions of FOXP3(+) CD25(high) T cells in neonates are not protective against later sensitization or development of allergy.

View Article: PubMed Central - PubMed

Affiliation: Department of Rheumatology and Inflammation Research, The Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden.

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Related in: MedlinePlus

(a and b) The proportions of FOXP3+CD25high cells within theCD4+ T cell population at birth and at 3–5 days in children who weresensitized, that is, specific IgE to food and/or inhalant allergens, or non-sensitized at (a) 18 or(b) 36 months of age. (c and d) The proportions ofCTLA-4+CD25+ cells within the CD4+ T cellpopulation at birth and at 3–5 days in children who were sensitized or non-sensitizedat (c) 18 or (d) 36 months of age. Each dot represents an individual, and horizontal barsindicate median value. Statistical differences between the groups were calculated using two-tailedMann–Whitney U-test.P ≤ 0.05 was regarded as significant(*P ≤ 0.05; **P ≤ 0.01; and ***P ≤ 0.001). (e) Gating strategies for FOXP3or CTLA-4 expression within the various CD25+ T cell populations in cord blood.Numbers represent the percentage of cells within the gate.
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fig02: (a and b) The proportions of FOXP3+CD25high cells within theCD4+ T cell population at birth and at 3–5 days in children who weresensitized, that is, specific IgE to food and/or inhalant allergens, or non-sensitized at (a) 18 or(b) 36 months of age. (c and d) The proportions ofCTLA-4+CD25+ cells within the CD4+ T cellpopulation at birth and at 3–5 days in children who were sensitized or non-sensitizedat (c) 18 or (d) 36 months of age. Each dot represents an individual, and horizontal barsindicate median value. Statistical differences between the groups were calculated using two-tailedMann–Whitney U-test.P ≤ 0.05 was regarded as significant(*P ≤ 0.05; **P ≤ 0.01; and ***P ≤ 0.001). (e) Gating strategies for FOXP3or CTLA-4 expression within the various CD25+ T cell populations in cord blood.Numbers represent the percentage of cells within the gate.

Mentions: Univariate analyses demonstrate that children who were sensitized at 18 or 36 months ofage had significantly higher proportions of FOXP3+CD25high cells withinthe CD4+ T cell population at birth and at 3 days of life thannon-sensitized children (Figs2a and b). Despite the factthat Tregs are considered to express the immunoregulatory molecule CTLA-4 25,26, the proportions ofCTLA-4+CD25+ T cells were unrelated to sensitization in bothmultivariate (Figs1c and d) and univariate analyses(Figs2c and d). The gating strategies forFOXP3+CD25high and CTLA-4+CD25+expression within the CD4+ T cell population are demonstrated in Fig.2e.


High proportions of FOXP3(+) CD25(high) T cells in neonates are positively associated with allergic sensitization later in childhood.

Strömbeck A, Rabe H, Lundell AC, Andersson K, Johansen S, Adlerberth I, Wold AE, Hesselmar B, Rudin A - Clin. Exp. Allergy (2014)

(a and b) The proportions of FOXP3+CD25high cells within theCD4+ T cell population at birth and at 3–5 days in children who weresensitized, that is, specific IgE to food and/or inhalant allergens, or non-sensitized at (a) 18 or(b) 36 months of age. (c and d) The proportions ofCTLA-4+CD25+ cells within the CD4+ T cellpopulation at birth and at 3–5 days in children who were sensitized or non-sensitizedat (c) 18 or (d) 36 months of age. Each dot represents an individual, and horizontal barsindicate median value. Statistical differences between the groups were calculated using two-tailedMann–Whitney U-test.P ≤ 0.05 was regarded as significant(*P ≤ 0.05; **P ≤ 0.01; and ***P ≤ 0.001). (e) Gating strategies for FOXP3or CTLA-4 expression within the various CD25+ T cell populations in cord blood.Numbers represent the percentage of cells within the gate.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4215110&req=5

fig02: (a and b) The proportions of FOXP3+CD25high cells within theCD4+ T cell population at birth and at 3–5 days in children who weresensitized, that is, specific IgE to food and/or inhalant allergens, or non-sensitized at (a) 18 or(b) 36 months of age. (c and d) The proportions ofCTLA-4+CD25+ cells within the CD4+ T cellpopulation at birth and at 3–5 days in children who were sensitized or non-sensitizedat (c) 18 or (d) 36 months of age. Each dot represents an individual, and horizontal barsindicate median value. Statistical differences between the groups were calculated using two-tailedMann–Whitney U-test.P ≤ 0.05 was regarded as significant(*P ≤ 0.05; **P ≤ 0.01; and ***P ≤ 0.001). (e) Gating strategies for FOXP3or CTLA-4 expression within the various CD25+ T cell populations in cord blood.Numbers represent the percentage of cells within the gate.
Mentions: Univariate analyses demonstrate that children who were sensitized at 18 or 36 months ofage had significantly higher proportions of FOXP3+CD25high cells withinthe CD4+ T cell population at birth and at 3 days of life thannon-sensitized children (Figs2a and b). Despite the factthat Tregs are considered to express the immunoregulatory molecule CTLA-4 25,26, the proportions ofCTLA-4+CD25+ T cells were unrelated to sensitization in bothmultivariate (Figs1c and d) and univariate analyses(Figs2c and d). The gating strategies forFOXP3+CD25high and CTLA-4+CD25+expression within the CD4+ T cell population are demonstrated in Fig.2e.

Bottom Line: The association between higher proportions of FOXP3(+) CD25(high) T cells and sensitization persisted after exclusion of farmer's children.Finally, a farming environment was associated with lower proportions of FOXP3(+) CD25(high) T cells in early infancy and to a more prominent T cell memory conversion and cytokine production.Our results indicate that high proportions of FOXP3(+) CD25(high) T cells in neonates are not protective against later sensitization or development of allergy.

View Article: PubMed Central - PubMed

Affiliation: Department of Rheumatology and Inflammation Research, The Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden.

Show MeSH
Related in: MedlinePlus