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Synergistic effects of Bifidobacterium thermophilum RBL67 and selected prebiotics on inhibition of Salmonella colonization in the swine proximal colon PolyFermS model.

Tanner SA, Chassard C, Zihler Berner A, Lacroix C - Gut Pathog (2014)

Bottom Line: Our study demonstrates the suitability of the porcine PolyFermS in vitro model to study nutritional effects of pro- and prebiotics on gut microbiota composition and activity.The inhibition effects of FOS and GOS on N-15 colonization are partly due to an increased acetate production, while further antimicrobial mechanisms may contribute to an enhanced inhibition with prebiotic-RBL67 combinations.A future direction of this work could be to understand the anti-Salmonella effects of Bifidobacterium thermophilum RBL67 in the presence of prebiotics to unravel the mechanism of this probiotic:pathogen interaction.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Food Biotechnology, Institute of Food, Nutrition and Health, Department of Health Science and Technology, ETH Zurich, Schmelzbergstrasse 7, 8092 Zurich, Switzerland.

ABSTRACT

Background: Probiotics and prebiotics are promising strategies to counteract Salmonella prevalence in swine. In the present study, we investigated the effects of prebiotics (fructo- (FOS), galacto- (GOS) and mannan- (MOS) oligosaccharides) and the bacteriocinogenic Bifidobacterium thermophilum RBL67 (RBL67) on Salmonella enterica subsp. enterica serovar Typhimurium N-15 (N-15) colonization using the PolyFermS in vitro continuous fermentation model simulating the swine proximal colon.

Material and methods: The PolyFermS model was designed with a first-stage reactor containing immobilized fecal pig microbiota. This reactor continuously inoculated five parallel second-stage reactors, a control and four treatment reactors, all operated with proximal colon conditions. FOS and GOS (5.2 g/day), and MOS (half dosage) and RBL67 (10(8) copy numbers/mL applied daily) were tested on the ability of N-15 to colonize reactors, inoculated with the same microbiota. Reactor effluents were collected daily and analyzed for microbial composition (quantitative PCR and 454 pyrosequencing of 16S rRNA gene pool) and main metabolites (HPLC).

Results: RBL67 and N-15 were shown to stably colonize the system. Colonization of N-15 was strongly inhibited by FOS and GOS, whereas addition of RBL67 alone or combined with MOS showed intermediate results. However, the effect of FOS and GOS was enhanced when prebiotics were combined with a daily addition of RBL67. FOS and GOS increased the total short chain fatty acid production, especially acetate and propionate. RBL67 combined with FOS additionally stimulated butyrate production.

Conclusions: Our study demonstrates the suitability of the porcine PolyFermS in vitro model to study nutritional effects of pro- and prebiotics on gut microbiota composition and activity. It can further be used to monitor Salmonella colonization. The inhibition effects of FOS and GOS on N-15 colonization are partly due to an increased acetate production, while further antimicrobial mechanisms may contribute to an enhanced inhibition with prebiotic-RBL67 combinations. A future direction of this work could be to understand the anti-Salmonella effects of Bifidobacterium thermophilum RBL67 in the presence of prebiotics to unravel the mechanism of this probiotic:pathogen interaction.

No MeSH data available.


Experimental set-up of the continuous fermentation experiment. IR: inoculum reactor; CR: control reactor; TR: test reactors 1–4; F: flow rate; M: fresh medium inflow; stab: stabilization; prev: prevention; challenge: challenge with Salmonella N-15; N-15: S. Typhimurium N-15; RBL67: B. thermophilum RBL67; R-FOS/GOS/MOS: B. thermophilum RBL67 + respective prebiotic.
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Fig1: Experimental set-up of the continuous fermentation experiment. IR: inoculum reactor; CR: control reactor; TR: test reactors 1–4; F: flow rate; M: fresh medium inflow; stab: stabilization; prev: prevention; challenge: challenge with Salmonella N-15; N-15: S. Typhimurium N-15; RBL67: B. thermophilum RBL67; R-FOS/GOS/MOS: B. thermophilum RBL67 + respective prebiotic.

Mentions: To evaluate the colonization ability of RBL67 and N-15 in an in vitro model of the swine proximal colon, we inoculated TRs once with RBL67 with and without FOS or with N-15 during period 1 (Figure 1). RBL67 and N-15 concentrations were estimated 96 h after addition and data were compared to the theoretical washout curve (Figure 2).Figure 1


Synergistic effects of Bifidobacterium thermophilum RBL67 and selected prebiotics on inhibition of Salmonella colonization in the swine proximal colon PolyFermS model.

Tanner SA, Chassard C, Zihler Berner A, Lacroix C - Gut Pathog (2014)

Experimental set-up of the continuous fermentation experiment. IR: inoculum reactor; CR: control reactor; TR: test reactors 1–4; F: flow rate; M: fresh medium inflow; stab: stabilization; prev: prevention; challenge: challenge with Salmonella N-15; N-15: S. Typhimurium N-15; RBL67: B. thermophilum RBL67; R-FOS/GOS/MOS: B. thermophilum RBL67 + respective prebiotic.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4215022&req=5

Fig1: Experimental set-up of the continuous fermentation experiment. IR: inoculum reactor; CR: control reactor; TR: test reactors 1–4; F: flow rate; M: fresh medium inflow; stab: stabilization; prev: prevention; challenge: challenge with Salmonella N-15; N-15: S. Typhimurium N-15; RBL67: B. thermophilum RBL67; R-FOS/GOS/MOS: B. thermophilum RBL67 + respective prebiotic.
Mentions: To evaluate the colonization ability of RBL67 and N-15 in an in vitro model of the swine proximal colon, we inoculated TRs once with RBL67 with and without FOS or with N-15 during period 1 (Figure 1). RBL67 and N-15 concentrations were estimated 96 h after addition and data were compared to the theoretical washout curve (Figure 2).Figure 1

Bottom Line: Our study demonstrates the suitability of the porcine PolyFermS in vitro model to study nutritional effects of pro- and prebiotics on gut microbiota composition and activity.The inhibition effects of FOS and GOS on N-15 colonization are partly due to an increased acetate production, while further antimicrobial mechanisms may contribute to an enhanced inhibition with prebiotic-RBL67 combinations.A future direction of this work could be to understand the anti-Salmonella effects of Bifidobacterium thermophilum RBL67 in the presence of prebiotics to unravel the mechanism of this probiotic:pathogen interaction.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Food Biotechnology, Institute of Food, Nutrition and Health, Department of Health Science and Technology, ETH Zurich, Schmelzbergstrasse 7, 8092 Zurich, Switzerland.

ABSTRACT

Background: Probiotics and prebiotics are promising strategies to counteract Salmonella prevalence in swine. In the present study, we investigated the effects of prebiotics (fructo- (FOS), galacto- (GOS) and mannan- (MOS) oligosaccharides) and the bacteriocinogenic Bifidobacterium thermophilum RBL67 (RBL67) on Salmonella enterica subsp. enterica serovar Typhimurium N-15 (N-15) colonization using the PolyFermS in vitro continuous fermentation model simulating the swine proximal colon.

Material and methods: The PolyFermS model was designed with a first-stage reactor containing immobilized fecal pig microbiota. This reactor continuously inoculated five parallel second-stage reactors, a control and four treatment reactors, all operated with proximal colon conditions. FOS and GOS (5.2 g/day), and MOS (half dosage) and RBL67 (10(8) copy numbers/mL applied daily) were tested on the ability of N-15 to colonize reactors, inoculated with the same microbiota. Reactor effluents were collected daily and analyzed for microbial composition (quantitative PCR and 454 pyrosequencing of 16S rRNA gene pool) and main metabolites (HPLC).

Results: RBL67 and N-15 were shown to stably colonize the system. Colonization of N-15 was strongly inhibited by FOS and GOS, whereas addition of RBL67 alone or combined with MOS showed intermediate results. However, the effect of FOS and GOS was enhanced when prebiotics were combined with a daily addition of RBL67. FOS and GOS increased the total short chain fatty acid production, especially acetate and propionate. RBL67 combined with FOS additionally stimulated butyrate production.

Conclusions: Our study demonstrates the suitability of the porcine PolyFermS in vitro model to study nutritional effects of pro- and prebiotics on gut microbiota composition and activity. It can further be used to monitor Salmonella colonization. The inhibition effects of FOS and GOS on N-15 colonization are partly due to an increased acetate production, while further antimicrobial mechanisms may contribute to an enhanced inhibition with prebiotic-RBL67 combinations. A future direction of this work could be to understand the anti-Salmonella effects of Bifidobacterium thermophilum RBL67 in the presence of prebiotics to unravel the mechanism of this probiotic:pathogen interaction.

No MeSH data available.