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High Prevalence of Class 1 to 3 Integrons Among Multidrug-Resistant Diarrheagenic Escherichia coli in Southwest of Iran.

Kargar M, Mohammadalipour Z, Doosti A, Lorzadeh S, Japoni-Nejad A - Osong Public Health Res Perspect (2014)

Bottom Line: For the first time in Iran, class 3 integron was observed in 26.09% MDR isolates.Our results indicate that integrons are common among MDR isolates and they can be used as a marker for the identification of MDR isolates.Therefore, due to the possibility of a widespread outbreak of MDR isolates, molecular surveillance and sequencing of the integrons in other parts of the country is recommended.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran.

ABSTRACT

Objectives: Horizontal transfer of integrons is one of the important factors that can contribute to the occurrence of multidrug-resistant (MDR) bacteria. This study aimed to determine the prevalence of integrons among MDR Escherichia coli strains isolated from stool specimens and investigate the associations between the existence of integrons and MDR properties in the southwest of Iran.

Methods: There were 164 E. coli strains isolated from January 2012 to June 2012. Fecal specimens identified as E. coli by the conventional methods. Subsequently the antibiotic resistance was assessed using Clinical and Laboratory Standard Institute criteria. The presence of class 1-3 integrons and embedded gene cassettes was verified using specific primers by multiplex polymerase chain reaction assay.

Results: Among a total of 164 studied samples, 69 (42.07%) isolates were multidrug resistant. Class 1 and class 2 integrons were present in 78.26% and 76.81% MDR isolates, respectively. For the first time in Iran, class 3 integron was observed in 26.09% MDR isolates. Significant correlations were identified between: class 1 integron and resistance to amikacin, gentamicin, chloramphenicol, ampicillin, tetracycline, nalidixic acid, and co-trimoxazole; class 2 integron and resistance to aminoglycosides, co-trimoxazole, cefalexin, ampicillin, and chloramphenicol; and class 3 integron and resistance to gentamicin, kanamycin, and streptomycin.

Conclusion: Our results indicate that integrons are common among MDR isolates and they can be used as a marker for the identification of MDR isolates. Therefore, due to the possibility of a widespread outbreak of MDR isolates, molecular surveillance and sequencing of the integrons in other parts of the country is recommended.

No MeSH data available.


Related in: MedlinePlus

The percentages of antimicrobial resistance detected among multidrug-resistant E. coli isolates.
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fig1: The percentages of antimicrobial resistance detected among multidrug-resistant E. coli isolates.

Mentions: E. coli isolates were collected from 164 clinical specimens of patients referred to Yasouj Hospital. The isolates were obtained from children aged <5 years. Sixty-nine isolated strains (42.07%) were designated as MDR, with 23 (33.33%) isolates from female and 46 (66.67%) from male patients. The percentage of the resistant isolates to the tested antimicrobials is presented in Figure 1. Of the 69 MDR E. coli isolates, 54 (78.26%) isolates were identified as being positive for class 1 integron. PCR amplification of the integron cassette region occurred in 48 (69.56%) class 1 integron-containing isolates (Table 2). Class 2 integron was detected in 53 (76.81%) isolates. The integron cassette region could not be amplified by PCR in 24 (45.28%) of the class 2 integron-containing isolates (Table 3). The frequency of simultaneous occurrence of integrons is depicted in Figure 2. For the first time in Iran, class 3 integron was observed in 18 (26.09%) isolates. A significant correlation was revealed between class 1 integron and the gene cassettes with resistance to amikacin (p = 0.027), gentamicin (p = 0.040, p = 0.001), chloramphenicol (p = 0.026), ampicillin (p = 0.018, p = 0.000), tetracycline (p < 0.001), nalidixic acid (p = 0.026), and co-trimoxazole (p = 0.032, p = 0.015); and also between class 2 integron and gene cassette with resistance to kanamycin (p = 0.006), streptomycin (p = 0.006), amikacin (p = 0.005), gentamicin (p = 0.000), cefalexin (p = 0.029), co-trimoxazole (p = 0.021), ampicillin (p = 0.029), and chloramphenicol (p = 0.009). In addition, a significant association was found between class 3 integron and resistance to gentamicin (p = 0.031), kanamycin (p = 0.039), and streptomycin (p = 0.007).


High Prevalence of Class 1 to 3 Integrons Among Multidrug-Resistant Diarrheagenic Escherichia coli in Southwest of Iran.

Kargar M, Mohammadalipour Z, Doosti A, Lorzadeh S, Japoni-Nejad A - Osong Public Health Res Perspect (2014)

The percentages of antimicrobial resistance detected among multidrug-resistant E. coli isolates.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4215003&req=5

fig1: The percentages of antimicrobial resistance detected among multidrug-resistant E. coli isolates.
Mentions: E. coli isolates were collected from 164 clinical specimens of patients referred to Yasouj Hospital. The isolates were obtained from children aged <5 years. Sixty-nine isolated strains (42.07%) were designated as MDR, with 23 (33.33%) isolates from female and 46 (66.67%) from male patients. The percentage of the resistant isolates to the tested antimicrobials is presented in Figure 1. Of the 69 MDR E. coli isolates, 54 (78.26%) isolates were identified as being positive for class 1 integron. PCR amplification of the integron cassette region occurred in 48 (69.56%) class 1 integron-containing isolates (Table 2). Class 2 integron was detected in 53 (76.81%) isolates. The integron cassette region could not be amplified by PCR in 24 (45.28%) of the class 2 integron-containing isolates (Table 3). The frequency of simultaneous occurrence of integrons is depicted in Figure 2. For the first time in Iran, class 3 integron was observed in 18 (26.09%) isolates. A significant correlation was revealed between class 1 integron and the gene cassettes with resistance to amikacin (p = 0.027), gentamicin (p = 0.040, p = 0.001), chloramphenicol (p = 0.026), ampicillin (p = 0.018, p = 0.000), tetracycline (p < 0.001), nalidixic acid (p = 0.026), and co-trimoxazole (p = 0.032, p = 0.015); and also between class 2 integron and gene cassette with resistance to kanamycin (p = 0.006), streptomycin (p = 0.006), amikacin (p = 0.005), gentamicin (p = 0.000), cefalexin (p = 0.029), co-trimoxazole (p = 0.021), ampicillin (p = 0.029), and chloramphenicol (p = 0.009). In addition, a significant association was found between class 3 integron and resistance to gentamicin (p = 0.031), kanamycin (p = 0.039), and streptomycin (p = 0.007).

Bottom Line: For the first time in Iran, class 3 integron was observed in 26.09% MDR isolates.Our results indicate that integrons are common among MDR isolates and they can be used as a marker for the identification of MDR isolates.Therefore, due to the possibility of a widespread outbreak of MDR isolates, molecular surveillance and sequencing of the integrons in other parts of the country is recommended.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran.

ABSTRACT

Objectives: Horizontal transfer of integrons is one of the important factors that can contribute to the occurrence of multidrug-resistant (MDR) bacteria. This study aimed to determine the prevalence of integrons among MDR Escherichia coli strains isolated from stool specimens and investigate the associations between the existence of integrons and MDR properties in the southwest of Iran.

Methods: There were 164 E. coli strains isolated from January 2012 to June 2012. Fecal specimens identified as E. coli by the conventional methods. Subsequently the antibiotic resistance was assessed using Clinical and Laboratory Standard Institute criteria. The presence of class 1-3 integrons and embedded gene cassettes was verified using specific primers by multiplex polymerase chain reaction assay.

Results: Among a total of 164 studied samples, 69 (42.07%) isolates were multidrug resistant. Class 1 and class 2 integrons were present in 78.26% and 76.81% MDR isolates, respectively. For the first time in Iran, class 3 integron was observed in 26.09% MDR isolates. Significant correlations were identified between: class 1 integron and resistance to amikacin, gentamicin, chloramphenicol, ampicillin, tetracycline, nalidixic acid, and co-trimoxazole; class 2 integron and resistance to aminoglycosides, co-trimoxazole, cefalexin, ampicillin, and chloramphenicol; and class 3 integron and resistance to gentamicin, kanamycin, and streptomycin.

Conclusion: Our results indicate that integrons are common among MDR isolates and they can be used as a marker for the identification of MDR isolates. Therefore, due to the possibility of a widespread outbreak of MDR isolates, molecular surveillance and sequencing of the integrons in other parts of the country is recommended.

No MeSH data available.


Related in: MedlinePlus