Limits...
The Recency Period for Estimation of Human Immunodeficiency Virus Incidence by the AxSYM Avidity Assay and BED-Capture Enzyme Immunoassay in the Republic of Korea.

Yu HK, Heo TY, Kim NY, Wang JS, Lee JK, Kim SS, Kee MK - Osong Public Health Res Perspect (2014)

Bottom Line: We determined the recency period to estimate HIV incidence in Korea.These data showed that the nonparametric survival analysis often led to shorter recency periods than analysis of sensitivity and specificity as a new method.These findings suggest that more data from seroconverters and other methodologies are needed to determine the recency period for estimating HIV incidence.

View Article: PubMed Central - PubMed

Affiliation: Division of AIDS, Korea National Institute of Health, Cheongju, Korea.

ABSTRACT

Objectives: Measurement of the incidence of the human immunodeficiency virus (HIV) is very important for epidemiological studies. Here, we determined the recency period with the AxSYM avidity assay and the BED-capture enzyme immunoassay (BED-CEIA) in Korean seroconverters.

Methods: Two hundred longitudinal specimens from 81 seroconverters with incident HIV infections that had been collected at the Korea National Institute of Health were subjected to the AxSYM avidity assay (cutoff = 0.8) and BED-CEIA (cutoff = 0.8). The statistical method used to estimate the recency period in recent HIV infections was nonparametric survival analyses. Sensitivity and specificity were calculated for 10-day increments from 120 days to 230 days to determine the recency period.

Results: The mean recency period of the avidity assay and BED-CEIA using a survival method was 158 days [95% confidence interval (CI), 135-181 days] and 189 days (95% CI, 170-208 days), respectively. Based on the use of sensitivity and specificity, the mean recency period for the avidity assay and BED-CEIA was 150 days and 200 days, respectively.

Conclusion: We determined the recency period to estimate HIV incidence in Korea. These data showed that the nonparametric survival analysis often led to shorter recency periods than analysis of sensitivity and specificity as a new method. These findings suggest that more data from seroconverters and other methodologies are needed to determine the recency period for estimating HIV incidence.

No MeSH data available.


Related in: MedlinePlus

Distribution in results of the AxSYM avidity index and BED-capture enzyme immunoassay by human immunodeficiency virus seroconverters.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4214998&req=5

fig1: Distribution in results of the AxSYM avidity index and BED-capture enzyme immunoassay by human immunodeficiency virus seroconverters.

Mentions: Figure 1 shows how the results of the avidity assay and BED-CEIA changed in each of the 81 test patients in relation to time since HIV infection. The avidity assay was carried out on 191 specimens from 79 patients after excluding two patients with only one longitudinal observation each. All 200 specimens from 81 patients were tested with the BED-CEIA. Table 2 contains the results of Kaplan–Meier analyses undertaken on each set of assay results to estimate the recency period. The recency period of the avidity assay was 158 days [95% confidence interval (CI); 135–181 days]. The recency period of the BED-CEIA was estimated to be 189 days (95% CI; 170–208 days).


The Recency Period for Estimation of Human Immunodeficiency Virus Incidence by the AxSYM Avidity Assay and BED-Capture Enzyme Immunoassay in the Republic of Korea.

Yu HK, Heo TY, Kim NY, Wang JS, Lee JK, Kim SS, Kee MK - Osong Public Health Res Perspect (2014)

Distribution in results of the AxSYM avidity index and BED-capture enzyme immunoassay by human immunodeficiency virus seroconverters.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214998&req=5

fig1: Distribution in results of the AxSYM avidity index and BED-capture enzyme immunoassay by human immunodeficiency virus seroconverters.
Mentions: Figure 1 shows how the results of the avidity assay and BED-CEIA changed in each of the 81 test patients in relation to time since HIV infection. The avidity assay was carried out on 191 specimens from 79 patients after excluding two patients with only one longitudinal observation each. All 200 specimens from 81 patients were tested with the BED-CEIA. Table 2 contains the results of Kaplan–Meier analyses undertaken on each set of assay results to estimate the recency period. The recency period of the avidity assay was 158 days [95% confidence interval (CI); 135–181 days]. The recency period of the BED-CEIA was estimated to be 189 days (95% CI; 170–208 days).

Bottom Line: We determined the recency period to estimate HIV incidence in Korea.These data showed that the nonparametric survival analysis often led to shorter recency periods than analysis of sensitivity and specificity as a new method.These findings suggest that more data from seroconverters and other methodologies are needed to determine the recency period for estimating HIV incidence.

View Article: PubMed Central - PubMed

Affiliation: Division of AIDS, Korea National Institute of Health, Cheongju, Korea.

ABSTRACT

Objectives: Measurement of the incidence of the human immunodeficiency virus (HIV) is very important for epidemiological studies. Here, we determined the recency period with the AxSYM avidity assay and the BED-capture enzyme immunoassay (BED-CEIA) in Korean seroconverters.

Methods: Two hundred longitudinal specimens from 81 seroconverters with incident HIV infections that had been collected at the Korea National Institute of Health were subjected to the AxSYM avidity assay (cutoff = 0.8) and BED-CEIA (cutoff = 0.8). The statistical method used to estimate the recency period in recent HIV infections was nonparametric survival analyses. Sensitivity and specificity were calculated for 10-day increments from 120 days to 230 days to determine the recency period.

Results: The mean recency period of the avidity assay and BED-CEIA using a survival method was 158 days [95% confidence interval (CI), 135-181 days] and 189 days (95% CI, 170-208 days), respectively. Based on the use of sensitivity and specificity, the mean recency period for the avidity assay and BED-CEIA was 150 days and 200 days, respectively.

Conclusion: We determined the recency period to estimate HIV incidence in Korea. These data showed that the nonparametric survival analysis often led to shorter recency periods than analysis of sensitivity and specificity as a new method. These findings suggest that more data from seroconverters and other methodologies are needed to determine the recency period for estimating HIV incidence.

No MeSH data available.


Related in: MedlinePlus