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The Serine Protease Inhibitor, 4-(2-aminoethyl) Benzene Sulfonyl Fluoride Hydrochloride, Reduces Allergic Inflammation in a House Dust Mite Allergic Rhinitis Mouse Model.

Kim BY, Park HR, Shin JH, Kim SW, Cho JH, Park YJ, Kim SW - Allergy Asthma Immunol Res (2014)

Bottom Line: Additionally, the percentage of CD4(+)CD25(+)Foxp3(+) T cells, IL-10 levels, and Foxp3 mRNA levels increased in the S and C groups compared with those in the Derf group (P<0.05).AEBSF treatment decreased the proteolytic activity in the S and C groups (P<0.05).Prophylactic and therapeutic treatment with AEBSF significantly reduces allergic airway inflammation and can induce regulatory T cells in a murine model of AR.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology-Head and Neck Surgery, The Catholic University of Korea, College of Medicine, Seoul, Korea.

ABSTRACT

Purpose: Serine protease inhibitors are involved in immune development, anti-inflammatory mechanisms, and tissue repair. In the present study, the serine protease inhibitor 4-(2-aminoethyl) benzene sulfonyl fluoride hydrochloride (AEBSF) was evaluated for its prophylactic and therapeutic applications in a mouse model of allergic rhinitis (AR).

Methods: BALB/c mice were divided into 5 groups: contol (CON), Dermatophagoides farinae (Derf), AR mice treated with AEBSF before sensitization (S), AR mice treated with AEBSF after challenge (C), and steroid groups. Derf was used as an allergen. AEBSF was administered before S or after C. Allergic symptom scores, eosinophil counts, proteolytic activity, interferon-γ, interleukin (IL)-10 levels and serum Derf-specific IgE levels were measured. T-bet, GATA-3, Foxp3, IL-13, and transforming growth factor (TGF)-β mRNA levels were determined using real-time polymerase chain reaction. CD4(+)CD25(+)Foxp3(+) T cells were assessed using flow cytometry.

Results: Symptom scores, serum Derf-specific IgE levels, GATA-3 mRNA levels, IL-13 mRNA levels, and tissue eosinophil counts decreased in both the S and C groups (P<0.05). Additionally, the percentage of CD4(+)CD25(+)Foxp3(+) T cells, IL-10 levels, and Foxp3 mRNA levels increased in the S and C groups compared with those in the Derf group (P<0.05). AEBSF treatment decreased the proteolytic activity in the S and C groups (P<0.05).

Conclusions: Prophylactic and therapeutic treatment with AEBSF significantly reduces allergic airway inflammation and can induce regulatory T cells in a murine model of AR.

No MeSH data available.


Related in: MedlinePlus

(A) Flow cytometric analysis of CD4+CD25+Foxp3+ T cell subsets. Representative fluorescence-activated cell sorting analysis in each group. RU, upper right quadrant, which represents CD4+CD25+Foxp3+ T cells. (B) The percentage of splenic mononuclear cells that were CD4+CD25+Foxp3+ T cells. Error bars represent standard deviations. *P<0.05 vs the Derf group; **P<0.05 vs the control group.
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Figure 8: (A) Flow cytometric analysis of CD4+CD25+Foxp3+ T cell subsets. Representative fluorescence-activated cell sorting analysis in each group. RU, upper right quadrant, which represents CD4+CD25+Foxp3+ T cells. (B) The percentage of splenic mononuclear cells that were CD4+CD25+Foxp3+ T cells. Error bars represent standard deviations. *P<0.05 vs the Derf group; **P<0.05 vs the control group.

Mentions: Cells were sorted based on Foxp3 and CD25 expression levels and whether they expressed CD4 (Fig. 8A). CD4+CD25+Foxp3+ T cells accounted for 0.065±0.026% of all splenic mononuclear cells in the CON group, 0.047±0.013% in the Derf group, 0.122±0.033% in the S group, 0.143±0.033% in the C group, and 0.07±0.028% in the steroid groups. The S and C groups had significantlyhigher percentages of these cells than the CON (S, P=0.008; C, P=0.000) and Derf (S, P=0.000; C, P=0.000) groups. The steroid group had a significantly higher percentage of CD4+CD25+Foxp3+ T cells than the Derf group (P=0.008) (Fig. 8B).


The Serine Protease Inhibitor, 4-(2-aminoethyl) Benzene Sulfonyl Fluoride Hydrochloride, Reduces Allergic Inflammation in a House Dust Mite Allergic Rhinitis Mouse Model.

Kim BY, Park HR, Shin JH, Kim SW, Cho JH, Park YJ, Kim SW - Allergy Asthma Immunol Res (2014)

(A) Flow cytometric analysis of CD4+CD25+Foxp3+ T cell subsets. Representative fluorescence-activated cell sorting analysis in each group. RU, upper right quadrant, which represents CD4+CD25+Foxp3+ T cells. (B) The percentage of splenic mononuclear cells that were CD4+CD25+Foxp3+ T cells. Error bars represent standard deviations. *P<0.05 vs the Derf group; **P<0.05 vs the control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4214977&req=5

Figure 8: (A) Flow cytometric analysis of CD4+CD25+Foxp3+ T cell subsets. Representative fluorescence-activated cell sorting analysis in each group. RU, upper right quadrant, which represents CD4+CD25+Foxp3+ T cells. (B) The percentage of splenic mononuclear cells that were CD4+CD25+Foxp3+ T cells. Error bars represent standard deviations. *P<0.05 vs the Derf group; **P<0.05 vs the control group.
Mentions: Cells were sorted based on Foxp3 and CD25 expression levels and whether they expressed CD4 (Fig. 8A). CD4+CD25+Foxp3+ T cells accounted for 0.065±0.026% of all splenic mononuclear cells in the CON group, 0.047±0.013% in the Derf group, 0.122±0.033% in the S group, 0.143±0.033% in the C group, and 0.07±0.028% in the steroid groups. The S and C groups had significantlyhigher percentages of these cells than the CON (S, P=0.008; C, P=0.000) and Derf (S, P=0.000; C, P=0.000) groups. The steroid group had a significantly higher percentage of CD4+CD25+Foxp3+ T cells than the Derf group (P=0.008) (Fig. 8B).

Bottom Line: Additionally, the percentage of CD4(+)CD25(+)Foxp3(+) T cells, IL-10 levels, and Foxp3 mRNA levels increased in the S and C groups compared with those in the Derf group (P<0.05).AEBSF treatment decreased the proteolytic activity in the S and C groups (P<0.05).Prophylactic and therapeutic treatment with AEBSF significantly reduces allergic airway inflammation and can induce regulatory T cells in a murine model of AR.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology-Head and Neck Surgery, The Catholic University of Korea, College of Medicine, Seoul, Korea.

ABSTRACT

Purpose: Serine protease inhibitors are involved in immune development, anti-inflammatory mechanisms, and tissue repair. In the present study, the serine protease inhibitor 4-(2-aminoethyl) benzene sulfonyl fluoride hydrochloride (AEBSF) was evaluated for its prophylactic and therapeutic applications in a mouse model of allergic rhinitis (AR).

Methods: BALB/c mice were divided into 5 groups: contol (CON), Dermatophagoides farinae (Derf), AR mice treated with AEBSF before sensitization (S), AR mice treated with AEBSF after challenge (C), and steroid groups. Derf was used as an allergen. AEBSF was administered before S or after C. Allergic symptom scores, eosinophil counts, proteolytic activity, interferon-γ, interleukin (IL)-10 levels and serum Derf-specific IgE levels were measured. T-bet, GATA-3, Foxp3, IL-13, and transforming growth factor (TGF)-β mRNA levels were determined using real-time polymerase chain reaction. CD4(+)CD25(+)Foxp3(+) T cells were assessed using flow cytometry.

Results: Symptom scores, serum Derf-specific IgE levels, GATA-3 mRNA levels, IL-13 mRNA levels, and tissue eosinophil counts decreased in both the S and C groups (P<0.05). Additionally, the percentage of CD4(+)CD25(+)Foxp3(+) T cells, IL-10 levels, and Foxp3 mRNA levels increased in the S and C groups compared with those in the Derf group (P<0.05). AEBSF treatment decreased the proteolytic activity in the S and C groups (P<0.05).

Conclusions: Prophylactic and therapeutic treatment with AEBSF significantly reduces allergic airway inflammation and can induce regulatory T cells in a murine model of AR.

No MeSH data available.


Related in: MedlinePlus