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The Serine Protease Inhibitor, 4-(2-aminoethyl) Benzene Sulfonyl Fluoride Hydrochloride, Reduces Allergic Inflammation in a House Dust Mite Allergic Rhinitis Mouse Model.

Kim BY, Park HR, Shin JH, Kim SW, Cho JH, Park YJ, Kim SW - Allergy Asthma Immunol Res (2014)

Bottom Line: Additionally, the percentage of CD4(+)CD25(+)Foxp3(+) T cells, IL-10 levels, and Foxp3 mRNA levels increased in the S and C groups compared with those in the Derf group (P<0.05).AEBSF treatment decreased the proteolytic activity in the S and C groups (P<0.05).Prophylactic and therapeutic treatment with AEBSF significantly reduces allergic airway inflammation and can induce regulatory T cells in a murine model of AR.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology-Head and Neck Surgery, The Catholic University of Korea, College of Medicine, Seoul, Korea.

ABSTRACT

Purpose: Serine protease inhibitors are involved in immune development, anti-inflammatory mechanisms, and tissue repair. In the present study, the serine protease inhibitor 4-(2-aminoethyl) benzene sulfonyl fluoride hydrochloride (AEBSF) was evaluated for its prophylactic and therapeutic applications in a mouse model of allergic rhinitis (AR).

Methods: BALB/c mice were divided into 5 groups: contol (CON), Dermatophagoides farinae (Derf), AR mice treated with AEBSF before sensitization (S), AR mice treated with AEBSF after challenge (C), and steroid groups. Derf was used as an allergen. AEBSF was administered before S or after C. Allergic symptom scores, eosinophil counts, proteolytic activity, interferon-γ, interleukin (IL)-10 levels and serum Derf-specific IgE levels were measured. T-bet, GATA-3, Foxp3, IL-13, and transforming growth factor (TGF)-β mRNA levels were determined using real-time polymerase chain reaction. CD4(+)CD25(+)Foxp3(+) T cells were assessed using flow cytometry.

Results: Symptom scores, serum Derf-specific IgE levels, GATA-3 mRNA levels, IL-13 mRNA levels, and tissue eosinophil counts decreased in both the S and C groups (P<0.05). Additionally, the percentage of CD4(+)CD25(+)Foxp3(+) T cells, IL-10 levels, and Foxp3 mRNA levels increased in the S and C groups compared with those in the Derf group (P<0.05). AEBSF treatment decreased the proteolytic activity in the S and C groups (P<0.05).

Conclusions: Prophylactic and therapeutic treatment with AEBSF significantly reduces allergic airway inflammation and can induce regulatory T cells in a murine model of AR.

No MeSH data available.


Related in: MedlinePlus

Quantitative analysis of T-bet (A), GATA-3 (B), and Foxp3 (C) mRNA levels in splenic mononuclear cells by real-time PCR. IL-13 (D) and TGF-β (E) mRNA levels in nasal mucosa. The results were normalized to GAPDH expression. Error bars represent standard deviations. *P<0.05 vs the Derf group; **P<0.05 vs the control group.
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Figure 7: Quantitative analysis of T-bet (A), GATA-3 (B), and Foxp3 (C) mRNA levels in splenic mononuclear cells by real-time PCR. IL-13 (D) and TGF-β (E) mRNA levels in nasal mucosa. The results were normalized to GAPDH expression. Error bars represent standard deviations. *P<0.05 vs the Derf group; **P<0.05 vs the control group.

Mentions: T-bet, GATA-3, and Foxp3 mRNA levels in splenic mononuclear cells were evaluated using real-time PCR. T-bet mRNA levels showed no significant differences among the groups (Fig. 7A). The GATA-3 mRNA level was significantly higher in the Derf group than in the CON (P=0.000), S (P=0.009), C, and steroid groups (all, P=0.000; Fig. 7B). Foxp3 mRNA levels were significantly higher in the S (P=0.000), C (P=0.000), and steroid (P=0.013) groups than in the Derf group (Fig. 7C). IL-13 mRNA levels were significantly higher in the Derf group than in the S (P=0.000), C (P=0.001), and steroid (P=0.001) groups (Fig. 7D). TGF-β mRNA levels showed no significant differences among the groups (Fig. 7E).


The Serine Protease Inhibitor, 4-(2-aminoethyl) Benzene Sulfonyl Fluoride Hydrochloride, Reduces Allergic Inflammation in a House Dust Mite Allergic Rhinitis Mouse Model.

Kim BY, Park HR, Shin JH, Kim SW, Cho JH, Park YJ, Kim SW - Allergy Asthma Immunol Res (2014)

Quantitative analysis of T-bet (A), GATA-3 (B), and Foxp3 (C) mRNA levels in splenic mononuclear cells by real-time PCR. IL-13 (D) and TGF-β (E) mRNA levels in nasal mucosa. The results were normalized to GAPDH expression. Error bars represent standard deviations. *P<0.05 vs the Derf group; **P<0.05 vs the control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214977&req=5

Figure 7: Quantitative analysis of T-bet (A), GATA-3 (B), and Foxp3 (C) mRNA levels in splenic mononuclear cells by real-time PCR. IL-13 (D) and TGF-β (E) mRNA levels in nasal mucosa. The results were normalized to GAPDH expression. Error bars represent standard deviations. *P<0.05 vs the Derf group; **P<0.05 vs the control group.
Mentions: T-bet, GATA-3, and Foxp3 mRNA levels in splenic mononuclear cells were evaluated using real-time PCR. T-bet mRNA levels showed no significant differences among the groups (Fig. 7A). The GATA-3 mRNA level was significantly higher in the Derf group than in the CON (P=0.000), S (P=0.009), C, and steroid groups (all, P=0.000; Fig. 7B). Foxp3 mRNA levels were significantly higher in the S (P=0.000), C (P=0.000), and steroid (P=0.013) groups than in the Derf group (Fig. 7C). IL-13 mRNA levels were significantly higher in the Derf group than in the S (P=0.000), C (P=0.001), and steroid (P=0.001) groups (Fig. 7D). TGF-β mRNA levels showed no significant differences among the groups (Fig. 7E).

Bottom Line: Additionally, the percentage of CD4(+)CD25(+)Foxp3(+) T cells, IL-10 levels, and Foxp3 mRNA levels increased in the S and C groups compared with those in the Derf group (P<0.05).AEBSF treatment decreased the proteolytic activity in the S and C groups (P<0.05).Prophylactic and therapeutic treatment with AEBSF significantly reduces allergic airway inflammation and can induce regulatory T cells in a murine model of AR.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology-Head and Neck Surgery, The Catholic University of Korea, College of Medicine, Seoul, Korea.

ABSTRACT

Purpose: Serine protease inhibitors are involved in immune development, anti-inflammatory mechanisms, and tissue repair. In the present study, the serine protease inhibitor 4-(2-aminoethyl) benzene sulfonyl fluoride hydrochloride (AEBSF) was evaluated for its prophylactic and therapeutic applications in a mouse model of allergic rhinitis (AR).

Methods: BALB/c mice were divided into 5 groups: contol (CON), Dermatophagoides farinae (Derf), AR mice treated with AEBSF before sensitization (S), AR mice treated with AEBSF after challenge (C), and steroid groups. Derf was used as an allergen. AEBSF was administered before S or after C. Allergic symptom scores, eosinophil counts, proteolytic activity, interferon-γ, interleukin (IL)-10 levels and serum Derf-specific IgE levels were measured. T-bet, GATA-3, Foxp3, IL-13, and transforming growth factor (TGF)-β mRNA levels were determined using real-time polymerase chain reaction. CD4(+)CD25(+)Foxp3(+) T cells were assessed using flow cytometry.

Results: Symptom scores, serum Derf-specific IgE levels, GATA-3 mRNA levels, IL-13 mRNA levels, and tissue eosinophil counts decreased in both the S and C groups (P<0.05). Additionally, the percentage of CD4(+)CD25(+)Foxp3(+) T cells, IL-10 levels, and Foxp3 mRNA levels increased in the S and C groups compared with those in the Derf group (P<0.05). AEBSF treatment decreased the proteolytic activity in the S and C groups (P<0.05).

Conclusions: Prophylactic and therapeutic treatment with AEBSF significantly reduces allergic airway inflammation and can induce regulatory T cells in a murine model of AR.

No MeSH data available.


Related in: MedlinePlus