Limits...
The effect of probiotics for preventing radiation-induced morphological changes in intestinal mucosa of rats.

Ki Y, Kim W, Cho H, Ahn K, Choi Y, Kim D - J. Korean Med. Sci. (2014)

Bottom Line: Probiotics is reported to have positive effects against radiation-induced enteropathy.There was no significant morphometric difference between two groups irradiated with 20 Gy of radiation.Probiotics appear to be effective for the morphological shortening of small intestinal mucosa damaged by radiation less than or equal to 15 Gy.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiation Oncology, Medical Research Institute, Pusan National University Hospital, Pusan National University School of Medicine, Busan, Korea.

ABSTRACT
Radiation therapy is an important treatment modality for abdominal or pelvic cancer, but there is a common and serious complication such as radiation-induced enteritis. Probiotics is reported to have positive effects against radiation-induced enteropathy. In this study, morphological changes of bowel mucosa were analyzed in rats to presume the effect of probiotics on radiation-induced enteritis and its correlation with radiation dose. A total of 48 adult male Sprague-Dawley rats were randomly assigned to two groups and received a solution containing 1.0×10(8) colony-forming units of Lactiobacillus acidophilus or water once daily for 10 days. Each of two groups was divided into three subgroups and abdomino-pelvic area of each subgroup was irradiated with 10, 15, and 20 Gy, respectively on the seventh day of feeding the solutions. All rats were sacrificed 3 days after irradiation and the mucosal thickness and villus height of jejunum, ileum and colon were measured. The morphological parameters of the small intestine represented significant differences between two solution groups irradiated 10 or 15 Gy, except for villus height of jejunum in 15 Gy-subgroup (P=0.065). There was no significant morphometric difference between two groups irradiated with 20 Gy of radiation. Probiotics appear to be effective for the morphological shortening of small intestinal mucosa damaged by radiation less than or equal to 15 Gy.

Show MeSH

Related in: MedlinePlus

Morphometric changes of mucosal thickness (A) and villus height (B) according to radiation dose in ileum.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4214937&req=5

Figure 4: Morphometric changes of mucosal thickness (A) and villus height (B) according to radiation dose in ileum.

Mentions: With the higher dose of radiation compared with the lower dose, there were significant decreases in mucosal thickness and villous height of ileum (P<0.001 for both; Fig. 4). There were significantly greater thickness of mucosa and height of villi in L. acidophilus group irradiated with 10 Gy than those in placebo group (P=0.028 and P=0.010, respectively; Table 2). The two histologic parameters of ileal segments after 15 Gy irradiation also presented significantly higher values in L. acidophilus group (P<0.001 for both). Fig. 3C and D show the histologic features in ileum after 15 Gy of irradiation. The rats irradiated with 20 Gy had no significant difference in morphometric analysis of ileal mucosa between L. acidophilus group and placebo group.


The effect of probiotics for preventing radiation-induced morphological changes in intestinal mucosa of rats.

Ki Y, Kim W, Cho H, Ahn K, Choi Y, Kim D - J. Korean Med. Sci. (2014)

Morphometric changes of mucosal thickness (A) and villus height (B) according to radiation dose in ileum.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214937&req=5

Figure 4: Morphometric changes of mucosal thickness (A) and villus height (B) according to radiation dose in ileum.
Mentions: With the higher dose of radiation compared with the lower dose, there were significant decreases in mucosal thickness and villous height of ileum (P<0.001 for both; Fig. 4). There were significantly greater thickness of mucosa and height of villi in L. acidophilus group irradiated with 10 Gy than those in placebo group (P=0.028 and P=0.010, respectively; Table 2). The two histologic parameters of ileal segments after 15 Gy irradiation also presented significantly higher values in L. acidophilus group (P<0.001 for both). Fig. 3C and D show the histologic features in ileum after 15 Gy of irradiation. The rats irradiated with 20 Gy had no significant difference in morphometric analysis of ileal mucosa between L. acidophilus group and placebo group.

Bottom Line: Probiotics is reported to have positive effects against radiation-induced enteropathy.There was no significant morphometric difference between two groups irradiated with 20 Gy of radiation.Probiotics appear to be effective for the morphological shortening of small intestinal mucosa damaged by radiation less than or equal to 15 Gy.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiation Oncology, Medical Research Institute, Pusan National University Hospital, Pusan National University School of Medicine, Busan, Korea.

ABSTRACT
Radiation therapy is an important treatment modality for abdominal or pelvic cancer, but there is a common and serious complication such as radiation-induced enteritis. Probiotics is reported to have positive effects against radiation-induced enteropathy. In this study, morphological changes of bowel mucosa were analyzed in rats to presume the effect of probiotics on radiation-induced enteritis and its correlation with radiation dose. A total of 48 adult male Sprague-Dawley rats were randomly assigned to two groups and received a solution containing 1.0×10(8) colony-forming units of Lactiobacillus acidophilus or water once daily for 10 days. Each of two groups was divided into three subgroups and abdomino-pelvic area of each subgroup was irradiated with 10, 15, and 20 Gy, respectively on the seventh day of feeding the solutions. All rats were sacrificed 3 days after irradiation and the mucosal thickness and villus height of jejunum, ileum and colon were measured. The morphological parameters of the small intestine represented significant differences between two solution groups irradiated 10 or 15 Gy, except for villus height of jejunum in 15 Gy-subgroup (P=0.065). There was no significant morphometric difference between two groups irradiated with 20 Gy of radiation. Probiotics appear to be effective for the morphological shortening of small intestinal mucosa damaged by radiation less than or equal to 15 Gy.

Show MeSH
Related in: MedlinePlus