Cdc1 removes the ethanolamine phosphate of the first mannose of GPI anchors and thereby facilitates the integration of GPI proteins into the yeast cell wall.
Bottom Line: We find that the essential CDC1 gene can be deleted in mcd4∆ cells, which do not attach EtN-P to mannose 1 of the GPI anchor, suggesting that Cdc1 removes the EtN-P added by Mcd4.Cdc1-314(ts) mutants do not accumulate GPI proteins in the ER but have a partial secretion block later in the secretory pathway.This suggests that the presumed transglycosidases Dfg5 and Dcw1 of cdc1-314(ts) transfer GPI proteins to cell wall β1,6-glucans inefficiently.
Affiliation: Department of Biology, University of Fribourg, CH-1700 Fribourg, Switzerland.Show MeSH
Mentions: As shown in Figure 3A, cdc1* was unable to grow at 30°C; its growth was restored by WT CDC1, but not cdc1D144A, which has a point mutation in the Mn2+-binding motif (Losev et al., 2008). The CDC1* and cdc1* (cdc1-314) query strains were both crossed in quadruplicate to a library of 5850 mutants comprising deletions for each of the nonessential genes and 879 DAmP alleles of essential genes (Breslow et al., 2008).
Affiliation: Department of Biology, University of Fribourg, CH-1700 Fribourg, Switzerland.