TACC3 is a microtubule plus end-tracking protein that promotes axon elongation and also regulates microtubule plus end dynamics in multiple embryonic cell types.
Bottom Line: Using high-resolution live-imaging data on tagged +TIPs, we show that TACC3 localizes to the extreme microtubule plus end, where it lies distal to the microtubule polymerization marker EB1 and directly overlaps with the microtubule polymerase XMAP215.TACC3 also plays a role in regulating XMAP215 stability and localizing XMAP215 to microtubule plus ends.Taken together, our results implicate TACC3 as a +TIP that functions with XMAP215 to regulate microtubule plus end dynamics.
Affiliation: Department of Biology, Boston College, Chestnut Hill, MA 02467.Show MeSH
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Mentions: We first performed immunostaining of fixed, cultured embryonic neural crest cells and neuronal growth cones, using an antibody specific to X. laevis TACC3. Within neural crest cells, we observed punctate staining throughout the entire cell (Figure 1, A–C), as well as staining at the centrosome (Figure 1, A′–C′), consistent with previous reports of TACC3 localization (Gergely et al., 2000b; Groisman et al., 2000). We also observed strong labeling within axons and growth cones (Figure 1, D–F), which has not been previously reported, and we confirmed TACC3 presence in neural tissue by Western blot of axon lysates (Figure 1 Supplement). These results suggest that TACC3 may play a role within the growth cone during axon outgrowth.
Affiliation: Department of Biology, Boston College, Chestnut Hill, MA 02467.