Interplay between phosphorylation and palmitoylation mediates plasma membrane targeting and sorting of GAP43.
Bottom Line: Plasma membrane association decreased the diffusion constant fourfold in neuritic shafts.Simulations confirmed that a combination of diffusion, dynamic plasma membrane interaction and active transport of a small fraction of GAP43 suffices for efficient sorting to growth cones.Our data demonstrate a complex interplay between phosphorylation and lipidation in mediating the localization of GAP43 in neuronal cells.
Affiliation: Department of Neurobiology, University of Osnabrück, 49076 Osnabrück, Germany.Show MeSH
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Mentions: Regulation of protein mobility by dynamic plasma membrane association may also contribute to the anchorage of proteins in specific compartments of a neuron, such as the growth cone. To test this hypothesis, we determined the retention of GAP43, the different phosphomutants, and the two reference proteins in the cell body, the process shaft, and the tip of processes using spatially restricted photoactivation experiments (Figure 5, top). Retention was quantified by determining the fraction of the fluorescent protein population in the activated segment 10 s after photoactivation (see Materials and Methods).
Affiliation: Department of Neurobiology, University of Osnabrück, 49076 Osnabrück, Germany.