Romancing mitosis and the mitotic apparatus.
Bottom Line: Although first described more than a century ago by early investigators such as E.Building upon those innovations, my laboratory and other contemporary investigators first charted the ultrastructure and molecular organization of mitosis and chromosome movement and the assembly and function of the cytoskeleton.This field of research remains a significant challenge for future investigators in cell biology and medicine.
Affiliation: Baylor College of Medicine, Molecular and Cellular Biology, Houston, TX 77030 email@example.com.Show MeSH
Mentions: The resources of Hsu's lab opened seemingly endless opportunities for me as the only team member trained in electron microscopy. I enjoyed early success in characterizing the structure and organization of specialized regions of mammalian chromosomes such as primary constrictions, centromere and kinetochore structures, and secondary constrictions, including nucleolar organizing regions and telomeres. Our most significant early accomplishment was to provide the first detailed EM images of the kinetochore on mammalian chromosomes (Brinkley and Stubblefield, 1966). Following our first publication describing the trilayered platelike structure and fibrous corona, similar observations have been widely reported on mitotic chromosomes of many eukaryotic organisms. Thus the design of the kinetochore (Figure 1) is widely conserved in eukaryotic cells. There is still much to be learned, however, about this specialized chromosomal component and its function in partitioning chromosomes and maintaining genomic and genetic stability. Many studies are currently underway worldwide.
Affiliation: Baylor College of Medicine, Molecular and Cellular Biology, Houston, TX 77030 firstname.lastname@example.org.