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Multiple NUCLEAR FACTOR Y transcription factors respond to abiotic stress in Brassica napus L.

Xu L, Lin Z, Tao Q, Liang M, Zhao G, Yin X, Fu R - PLoS ONE (2014)

Bottom Line: Several NF-Y subfamily members exhibited collinear expression patterns.The cis-element organization of BnNF-Ys was similar to that of Arabidopsis thaliana, and the promoter regions exhibited higher levels of nucleotide sequence identity with Brassica rapa than with Brassica oleracea.This work represents an entry point for investigating the roles of canola NF-Y proteins during abiotic stress responses and provides insight into the genetic evolution of Brassica NF-Ys.

View Article: PubMed Central - PubMed

Affiliation: College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.

ABSTRACT
Members of the plant NUCLEAR FACTOR Y (NF-Y) family are composed of the NF-YA, NF-YB, and NF-YC subunits. In Brassica napus (canola), each of these subunits forms a multimember subfamily. Plant NF-Ys were reported to be involved in several abiotic stresses. In this study, we demonstrated that multiple members of thirty three BnNF-Ys responded rapidly to salinity, drought, or ABA treatments. Transcripts of five BnNF-YAs, seven BnNF-YBs, and two BnNF-YCs were up-regulated by salinity stress, whereas the expression of thirteen BnNF-YAs, ten BnNF-YBs, and four BnNF-YCs were induced by drought stress. Under NaCl treatments, the expression of one BnNF-YA10 and four NF-YBs (BnNF-YB3, BnNF-YB7, BnNF-YB10, and BnNF-YB14) were greatly increased. Under PEG treatments, the expression levels of four NF-YAs (BnNF-YA9, BnNF-YA10, BnNF-YA11, and BnNF-YA12) and five NF-YBs (BnNF-YB1, BnNF-YB8, BnNF-YB10, BnNF-YB13, and BnNF-YB14) were greatly induced. The expression profiles of 20 of the 27 salinity- or drought-induced BnNF-Ys were also affected by ABA treatment. The expression levels of six NF-YAs (BnNF-YA1, BnNF-YA7, BnNF-YA8, BnNF-YA9, BnNF-YA10, and BnNF-YA12) and seven BnNF-YB members (BnNF-YB2, BnNF-YB3, BnNF-YB7, BnNF-YB10, BnNF-YB11, BnNF-YB13, and BnNF-YB14) and two NF-YC members (BnNF-YC2 and BnNF-YC3) were greatly up-regulated by ABA treatments. Only a few BnNF-Ys were inhibited by the above three treatments. Several NF-Y subfamily members exhibited collinear expression patterns. The promoters of all stress-responsive BnNF-Ys harbored at least two types of stress-related cis-elements, such as ABRE, DRE, MYB, or MYC. The cis-element organization of BnNF-Ys was similar to that of Arabidopsis thaliana, and the promoter regions exhibited higher levels of nucleotide sequence identity with Brassica rapa than with Brassica oleracea. This work represents an entry point for investigating the roles of canola NF-Y proteins during abiotic stress responses and provides insight into the genetic evolution of Brassica NF-Ys.

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Expression pattern of the BnNF-Y genes in the leaves and roots of plants exposed to 100 µM ABA.Transcript levels of BnNF-Y (A), BnNF-YB (B), and BnNF-YC (C) genes after exposure to 100µM ABA. Three-week-old canola plantlets were treated with 100µM ABA for 1h and 3h. Total RNA was extracted from leaves and roots for quantitative PCR (qRT-PCR) analysis. Transcript levels of each BnNF-Y were first normalized to those of the housekeeping gene 18S and then compared to levels at each time point in the control. Each data point represents the mean ±SE of three independent experiments. Significant differences between treated samples and untreated controls (same tissue only) are indicated by a single (P<0.05) or double (P<0.01) asterisk, according to Dunnett’s method of one-way ANOVA in SPSS. Expression levels in untreated samples (CK, 1-h or 3-h leaf and root samples) were arbitrarily set to 1.0.
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pone-0111354-g003: Expression pattern of the BnNF-Y genes in the leaves and roots of plants exposed to 100 µM ABA.Transcript levels of BnNF-Y (A), BnNF-YB (B), and BnNF-YC (C) genes after exposure to 100µM ABA. Three-week-old canola plantlets were treated with 100µM ABA for 1h and 3h. Total RNA was extracted from leaves and roots for quantitative PCR (qRT-PCR) analysis. Transcript levels of each BnNF-Y were first normalized to those of the housekeeping gene 18S and then compared to levels at each time point in the control. Each data point represents the mean ±SE of three independent experiments. Significant differences between treated samples and untreated controls (same tissue only) are indicated by a single (P<0.05) or double (P<0.01) asterisk, according to Dunnett’s method of one-way ANOVA in SPSS. Expression levels in untreated samples (CK, 1-h or 3-h leaf and root samples) were arbitrarily set to 1.0.

Mentions: Plants respond to abiotic stresses via both the ABA-dependent and ABA-independent pathways. Some NF-Ys were reported to be involved in ABA-dependent pathways, whereas others were not [11], [13], [15]. To determine whether canola NF-Ys function via the ABA pathway, we further examined the expression profiles of BnNF-Ys that were found to be responsive to salinity or drought stress (i.e., all BnNF-YA members and most BnNF-YBs and BnNF-YCs; Figs 1 and 2) under ABA treatments. All BnNF-YAs, except for BnNF-YA4/5 and BnNF-YA13, were responsive to 3-h of ABA treatment (Fig. 3A). The transcript levels of BnNF-YA1, -YA2, -YA3, -YA6, -YA7, -YA8, -YA9, -YA10 and BnNF-YA11 were elevated at 1h. Also, most NaCl- or PEG- responsive BnNF-YB members were induced by ABA treatments, with the exception of BnNF-YB1 and BnNF-YB9 (Fig. 3B). The transcript level of BnNF-YB2 was inhibited in leaves after 1h of ABA treatment. Interestingly, several BnNF-YBs (BnNF-YB2, -YB3, -YB7, -YB10, -YB11, -YB13, and -YB14) were greatly up-regulated by ABA treatment. Amongst the BnNF-YC members, only the transcripts of BnNF-YC2 and BnNF-YC5 were increased in response to ABA treatment, and the upregulation was greater in the roots than in the leaves (Fig. 3C). The transcript levels of BnNF-YC1 remained almost constant from 1h to 3h of ABA treatment in all tissues examined.


Multiple NUCLEAR FACTOR Y transcription factors respond to abiotic stress in Brassica napus L.

Xu L, Lin Z, Tao Q, Liang M, Zhao G, Yin X, Fu R - PLoS ONE (2014)

Expression pattern of the BnNF-Y genes in the leaves and roots of plants exposed to 100 µM ABA.Transcript levels of BnNF-Y (A), BnNF-YB (B), and BnNF-YC (C) genes after exposure to 100µM ABA. Three-week-old canola plantlets were treated with 100µM ABA for 1h and 3h. Total RNA was extracted from leaves and roots for quantitative PCR (qRT-PCR) analysis. Transcript levels of each BnNF-Y were first normalized to those of the housekeeping gene 18S and then compared to levels at each time point in the control. Each data point represents the mean ±SE of three independent experiments. Significant differences between treated samples and untreated controls (same tissue only) are indicated by a single (P<0.05) or double (P<0.01) asterisk, according to Dunnett’s method of one-way ANOVA in SPSS. Expression levels in untreated samples (CK, 1-h or 3-h leaf and root samples) were arbitrarily set to 1.0.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214726&req=5

pone-0111354-g003: Expression pattern of the BnNF-Y genes in the leaves and roots of plants exposed to 100 µM ABA.Transcript levels of BnNF-Y (A), BnNF-YB (B), and BnNF-YC (C) genes after exposure to 100µM ABA. Three-week-old canola plantlets were treated with 100µM ABA for 1h and 3h. Total RNA was extracted from leaves and roots for quantitative PCR (qRT-PCR) analysis. Transcript levels of each BnNF-Y were first normalized to those of the housekeeping gene 18S and then compared to levels at each time point in the control. Each data point represents the mean ±SE of three independent experiments. Significant differences between treated samples and untreated controls (same tissue only) are indicated by a single (P<0.05) or double (P<0.01) asterisk, according to Dunnett’s method of one-way ANOVA in SPSS. Expression levels in untreated samples (CK, 1-h or 3-h leaf and root samples) were arbitrarily set to 1.0.
Mentions: Plants respond to abiotic stresses via both the ABA-dependent and ABA-independent pathways. Some NF-Ys were reported to be involved in ABA-dependent pathways, whereas others were not [11], [13], [15]. To determine whether canola NF-Ys function via the ABA pathway, we further examined the expression profiles of BnNF-Ys that were found to be responsive to salinity or drought stress (i.e., all BnNF-YA members and most BnNF-YBs and BnNF-YCs; Figs 1 and 2) under ABA treatments. All BnNF-YAs, except for BnNF-YA4/5 and BnNF-YA13, were responsive to 3-h of ABA treatment (Fig. 3A). The transcript levels of BnNF-YA1, -YA2, -YA3, -YA6, -YA7, -YA8, -YA9, -YA10 and BnNF-YA11 were elevated at 1h. Also, most NaCl- or PEG- responsive BnNF-YB members were induced by ABA treatments, with the exception of BnNF-YB1 and BnNF-YB9 (Fig. 3B). The transcript level of BnNF-YB2 was inhibited in leaves after 1h of ABA treatment. Interestingly, several BnNF-YBs (BnNF-YB2, -YB3, -YB7, -YB10, -YB11, -YB13, and -YB14) were greatly up-regulated by ABA treatment. Amongst the BnNF-YC members, only the transcripts of BnNF-YC2 and BnNF-YC5 were increased in response to ABA treatment, and the upregulation was greater in the roots than in the leaves (Fig. 3C). The transcript levels of BnNF-YC1 remained almost constant from 1h to 3h of ABA treatment in all tissues examined.

Bottom Line: Several NF-Y subfamily members exhibited collinear expression patterns.The cis-element organization of BnNF-Ys was similar to that of Arabidopsis thaliana, and the promoter regions exhibited higher levels of nucleotide sequence identity with Brassica rapa than with Brassica oleracea.This work represents an entry point for investigating the roles of canola NF-Y proteins during abiotic stress responses and provides insight into the genetic evolution of Brassica NF-Ys.

View Article: PubMed Central - PubMed

Affiliation: College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.

ABSTRACT
Members of the plant NUCLEAR FACTOR Y (NF-Y) family are composed of the NF-YA, NF-YB, and NF-YC subunits. In Brassica napus (canola), each of these subunits forms a multimember subfamily. Plant NF-Ys were reported to be involved in several abiotic stresses. In this study, we demonstrated that multiple members of thirty three BnNF-Ys responded rapidly to salinity, drought, or ABA treatments. Transcripts of five BnNF-YAs, seven BnNF-YBs, and two BnNF-YCs were up-regulated by salinity stress, whereas the expression of thirteen BnNF-YAs, ten BnNF-YBs, and four BnNF-YCs were induced by drought stress. Under NaCl treatments, the expression of one BnNF-YA10 and four NF-YBs (BnNF-YB3, BnNF-YB7, BnNF-YB10, and BnNF-YB14) were greatly increased. Under PEG treatments, the expression levels of four NF-YAs (BnNF-YA9, BnNF-YA10, BnNF-YA11, and BnNF-YA12) and five NF-YBs (BnNF-YB1, BnNF-YB8, BnNF-YB10, BnNF-YB13, and BnNF-YB14) were greatly induced. The expression profiles of 20 of the 27 salinity- or drought-induced BnNF-Ys were also affected by ABA treatment. The expression levels of six NF-YAs (BnNF-YA1, BnNF-YA7, BnNF-YA8, BnNF-YA9, BnNF-YA10, and BnNF-YA12) and seven BnNF-YB members (BnNF-YB2, BnNF-YB3, BnNF-YB7, BnNF-YB10, BnNF-YB11, BnNF-YB13, and BnNF-YB14) and two NF-YC members (BnNF-YC2 and BnNF-YC3) were greatly up-regulated by ABA treatments. Only a few BnNF-Ys were inhibited by the above three treatments. Several NF-Y subfamily members exhibited collinear expression patterns. The promoters of all stress-responsive BnNF-Ys harbored at least two types of stress-related cis-elements, such as ABRE, DRE, MYB, or MYC. The cis-element organization of BnNF-Ys was similar to that of Arabidopsis thaliana, and the promoter regions exhibited higher levels of nucleotide sequence identity with Brassica rapa than with Brassica oleracea. This work represents an entry point for investigating the roles of canola NF-Y proteins during abiotic stress responses and provides insight into the genetic evolution of Brassica NF-Ys.

Show MeSH
Related in: MedlinePlus