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Multiple NUCLEAR FACTOR Y transcription factors respond to abiotic stress in Brassica napus L.

Xu L, Lin Z, Tao Q, Liang M, Zhao G, Yin X, Fu R - PLoS ONE (2014)

Bottom Line: Several NF-Y subfamily members exhibited collinear expression patterns.The cis-element organization of BnNF-Ys was similar to that of Arabidopsis thaliana, and the promoter regions exhibited higher levels of nucleotide sequence identity with Brassica rapa than with Brassica oleracea.This work represents an entry point for investigating the roles of canola NF-Y proteins during abiotic stress responses and provides insight into the genetic evolution of Brassica NF-Ys.

View Article: PubMed Central - PubMed

Affiliation: College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.

ABSTRACT
Members of the plant NUCLEAR FACTOR Y (NF-Y) family are composed of the NF-YA, NF-YB, and NF-YC subunits. In Brassica napus (canola), each of these subunits forms a multimember subfamily. Plant NF-Ys were reported to be involved in several abiotic stresses. In this study, we demonstrated that multiple members of thirty three BnNF-Ys responded rapidly to salinity, drought, or ABA treatments. Transcripts of five BnNF-YAs, seven BnNF-YBs, and two BnNF-YCs were up-regulated by salinity stress, whereas the expression of thirteen BnNF-YAs, ten BnNF-YBs, and four BnNF-YCs were induced by drought stress. Under NaCl treatments, the expression of one BnNF-YA10 and four NF-YBs (BnNF-YB3, BnNF-YB7, BnNF-YB10, and BnNF-YB14) were greatly increased. Under PEG treatments, the expression levels of four NF-YAs (BnNF-YA9, BnNF-YA10, BnNF-YA11, and BnNF-YA12) and five NF-YBs (BnNF-YB1, BnNF-YB8, BnNF-YB10, BnNF-YB13, and BnNF-YB14) were greatly induced. The expression profiles of 20 of the 27 salinity- or drought-induced BnNF-Ys were also affected by ABA treatment. The expression levels of six NF-YAs (BnNF-YA1, BnNF-YA7, BnNF-YA8, BnNF-YA9, BnNF-YA10, and BnNF-YA12) and seven BnNF-YB members (BnNF-YB2, BnNF-YB3, BnNF-YB7, BnNF-YB10, BnNF-YB11, BnNF-YB13, and BnNF-YB14) and two NF-YC members (BnNF-YC2 and BnNF-YC3) were greatly up-regulated by ABA treatments. Only a few BnNF-Ys were inhibited by the above three treatments. Several NF-Y subfamily members exhibited collinear expression patterns. The promoters of all stress-responsive BnNF-Ys harbored at least two types of stress-related cis-elements, such as ABRE, DRE, MYB, or MYC. The cis-element organization of BnNF-Ys was similar to that of Arabidopsis thaliana, and the promoter regions exhibited higher levels of nucleotide sequence identity with Brassica rapa than with Brassica oleracea. This work represents an entry point for investigating the roles of canola NF-Y proteins during abiotic stress responses and provides insight into the genetic evolution of Brassica NF-Ys.

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Expression pattern of the BnNF-Y genes in plants subjected to drought stress.The expression of BnNF-YA (A), BnNF-YB (B), and BnNF-YC (C) genes in the leaves and roots of plants exposed to 15% (w/v) PEG6000. Three-week-old canola seedlings were treated with 15% (w/v) PEG-6000 for 1 and 3h. Total RNA was extracted from leaves (L) and roots (R) for quantitative PCR (qRT-PCR) analysis. Transcript levels of each BnNF-Y were first normalized to those of the housekeeping gene 18S and then compared to each time-point control. Each data point represents the mean ±SE of three independent experiments. Significant differences between treated samples and untreated controls (same tissue only) are indicated by single (P<0.05) or double (P<0.01) asterisks, according to Dunnett’s method of one-way ANOVA in SPSS. Expression levels in untreated samples (CK, 1-h or 3-h leaf and root samples) were arbitrarily set to 1.0.
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pone-0111354-g002: Expression pattern of the BnNF-Y genes in plants subjected to drought stress.The expression of BnNF-YA (A), BnNF-YB (B), and BnNF-YC (C) genes in the leaves and roots of plants exposed to 15% (w/v) PEG6000. Three-week-old canola seedlings were treated with 15% (w/v) PEG-6000 for 1 and 3h. Total RNA was extracted from leaves (L) and roots (R) for quantitative PCR (qRT-PCR) analysis. Transcript levels of each BnNF-Y were first normalized to those of the housekeeping gene 18S and then compared to each time-point control. Each data point represents the mean ±SE of three independent experiments. Significant differences between treated samples and untreated controls (same tissue only) are indicated by single (P<0.05) or double (P<0.01) asterisks, according to Dunnett’s method of one-way ANOVA in SPSS. Expression levels in untreated samples (CK, 1-h or 3-h leaf and root samples) were arbitrarily set to 1.0.

Mentions: As shown in Fig. 2, all BnNF-YA members were responsive to PEG6000 treatments, which mimic drought stress, in leaf tissues. A 15% PEG treatment generally caused a 72% relative water content loss within 24h (Table S2). The expression levels of BnNF-YA10, BnNF-YA11 and BnNF-Y12 in leaves were dramatically up-regulated to more than four-fold the levels observed in the untreated control (Fig. 2A). BnNF-YA8, BnNF-YA9, BnNF-YA10 and BnNF-YA13 were induced in roots under drought stress. Four BnNF-YAs (BnNF-YA8, -YA9, -YA10, and -YA13) responded to drought stress both in leaves and roots, particularly up to 3h of treatment. The expression levels of five BnNF-YAs (BnNF-YA1, BnNF-YA2, -YA3, -YA4/5, and -YA6) decreased in leaves at 1h and increased at 3h.


Multiple NUCLEAR FACTOR Y transcription factors respond to abiotic stress in Brassica napus L.

Xu L, Lin Z, Tao Q, Liang M, Zhao G, Yin X, Fu R - PLoS ONE (2014)

Expression pattern of the BnNF-Y genes in plants subjected to drought stress.The expression of BnNF-YA (A), BnNF-YB (B), and BnNF-YC (C) genes in the leaves and roots of plants exposed to 15% (w/v) PEG6000. Three-week-old canola seedlings were treated with 15% (w/v) PEG-6000 for 1 and 3h. Total RNA was extracted from leaves (L) and roots (R) for quantitative PCR (qRT-PCR) analysis. Transcript levels of each BnNF-Y were first normalized to those of the housekeeping gene 18S and then compared to each time-point control. Each data point represents the mean ±SE of three independent experiments. Significant differences between treated samples and untreated controls (same tissue only) are indicated by single (P<0.05) or double (P<0.01) asterisks, according to Dunnett’s method of one-way ANOVA in SPSS. Expression levels in untreated samples (CK, 1-h or 3-h leaf and root samples) were arbitrarily set to 1.0.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214726&req=5

pone-0111354-g002: Expression pattern of the BnNF-Y genes in plants subjected to drought stress.The expression of BnNF-YA (A), BnNF-YB (B), and BnNF-YC (C) genes in the leaves and roots of plants exposed to 15% (w/v) PEG6000. Three-week-old canola seedlings were treated with 15% (w/v) PEG-6000 for 1 and 3h. Total RNA was extracted from leaves (L) and roots (R) for quantitative PCR (qRT-PCR) analysis. Transcript levels of each BnNF-Y were first normalized to those of the housekeeping gene 18S and then compared to each time-point control. Each data point represents the mean ±SE of three independent experiments. Significant differences between treated samples and untreated controls (same tissue only) are indicated by single (P<0.05) or double (P<0.01) asterisks, according to Dunnett’s method of one-way ANOVA in SPSS. Expression levels in untreated samples (CK, 1-h or 3-h leaf and root samples) were arbitrarily set to 1.0.
Mentions: As shown in Fig. 2, all BnNF-YA members were responsive to PEG6000 treatments, which mimic drought stress, in leaf tissues. A 15% PEG treatment generally caused a 72% relative water content loss within 24h (Table S2). The expression levels of BnNF-YA10, BnNF-YA11 and BnNF-Y12 in leaves were dramatically up-regulated to more than four-fold the levels observed in the untreated control (Fig. 2A). BnNF-YA8, BnNF-YA9, BnNF-YA10 and BnNF-YA13 were induced in roots under drought stress. Four BnNF-YAs (BnNF-YA8, -YA9, -YA10, and -YA13) responded to drought stress both in leaves and roots, particularly up to 3h of treatment. The expression levels of five BnNF-YAs (BnNF-YA1, BnNF-YA2, -YA3, -YA4/5, and -YA6) decreased in leaves at 1h and increased at 3h.

Bottom Line: Several NF-Y subfamily members exhibited collinear expression patterns.The cis-element organization of BnNF-Ys was similar to that of Arabidopsis thaliana, and the promoter regions exhibited higher levels of nucleotide sequence identity with Brassica rapa than with Brassica oleracea.This work represents an entry point for investigating the roles of canola NF-Y proteins during abiotic stress responses and provides insight into the genetic evolution of Brassica NF-Ys.

View Article: PubMed Central - PubMed

Affiliation: College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.

ABSTRACT
Members of the plant NUCLEAR FACTOR Y (NF-Y) family are composed of the NF-YA, NF-YB, and NF-YC subunits. In Brassica napus (canola), each of these subunits forms a multimember subfamily. Plant NF-Ys were reported to be involved in several abiotic stresses. In this study, we demonstrated that multiple members of thirty three BnNF-Ys responded rapidly to salinity, drought, or ABA treatments. Transcripts of five BnNF-YAs, seven BnNF-YBs, and two BnNF-YCs were up-regulated by salinity stress, whereas the expression of thirteen BnNF-YAs, ten BnNF-YBs, and four BnNF-YCs were induced by drought stress. Under NaCl treatments, the expression of one BnNF-YA10 and four NF-YBs (BnNF-YB3, BnNF-YB7, BnNF-YB10, and BnNF-YB14) were greatly increased. Under PEG treatments, the expression levels of four NF-YAs (BnNF-YA9, BnNF-YA10, BnNF-YA11, and BnNF-YA12) and five NF-YBs (BnNF-YB1, BnNF-YB8, BnNF-YB10, BnNF-YB13, and BnNF-YB14) were greatly induced. The expression profiles of 20 of the 27 salinity- or drought-induced BnNF-Ys were also affected by ABA treatment. The expression levels of six NF-YAs (BnNF-YA1, BnNF-YA7, BnNF-YA8, BnNF-YA9, BnNF-YA10, and BnNF-YA12) and seven BnNF-YB members (BnNF-YB2, BnNF-YB3, BnNF-YB7, BnNF-YB10, BnNF-YB11, BnNF-YB13, and BnNF-YB14) and two NF-YC members (BnNF-YC2 and BnNF-YC3) were greatly up-regulated by ABA treatments. Only a few BnNF-Ys were inhibited by the above three treatments. Several NF-Y subfamily members exhibited collinear expression patterns. The promoters of all stress-responsive BnNF-Ys harbored at least two types of stress-related cis-elements, such as ABRE, DRE, MYB, or MYC. The cis-element organization of BnNF-Ys was similar to that of Arabidopsis thaliana, and the promoter regions exhibited higher levels of nucleotide sequence identity with Brassica rapa than with Brassica oleracea. This work represents an entry point for investigating the roles of canola NF-Y proteins during abiotic stress responses and provides insight into the genetic evolution of Brassica NF-Ys.

Show MeSH