Limits...
Serotypes and genotypes of invasive Streptococcus pneumoniae before and after PCV10 implementation in southern Brazil.

Caierão J, Hawkins P, Sant'anna FH, da Cunha GR, d'Azevedo PA, McGee L, Dias C - PLoS ONE (2014)

Bottom Line: To reduce the burden of pneumococcal diseases, different formulations of pneumococcal conjugate vaccines (PCV) have been introduced in many countries.Seventy different sequence types [STs] were found, accounting for 9 clonal complexes [CCs] and 45 singletons.Non-susceptibility to penicillin was associated with serotype 19A, CC320.

View Article: PubMed Central - PubMed

Affiliation: Federal University of Health Science of Porto Alegre, Rio Grande do Sul, Brazil.

ABSTRACT
To reduce the burden of pneumococcal diseases, different formulations of pneumococcal conjugate vaccines (PCV) have been introduced in many countries. In Brazil, PCV10 has been available since 2010. We aimed to analyze the serotype and genetic composition of invasive pneumococci from Brazil in pre- and post-vaccination periods (2007-2012). Antibiotic susceptibility was determined and genotypes of macrolide and fluoroquinolone resistance were characterized. The genotypes of isolates of the most frequent serotypes were determined by multilocus sequence typing. The study included 325 isolates, which were primarily recovered from blood. The most common serotypes recovered were 14, 3, 4, 23F, 7F, 9V, 12F, 20, 19F, 8, 19A, and 5. Thirty-eight pneumococci (11.7%) were from children ≤5 years old. Considering the overall population, PCV10 and PCV13 serotype coverage was 50.1% and 64.9%, respectively. During the pre-vaccine period, isolates with serotypes belonging to the PVC10 represented 51.5% (100/194), whereas in the post vaccine they represented 48.0% (63/131). PCV13 serotypes represented 67.5% (131/194) and 59.2% (77/131) of total for pre- and post-vaccination periods, respectively. Seventy different sequence types [STs] were found, accounting for 9 clonal complexes [CCs] and 45 singletons. Eight STs (156, 180, 218, 8889, 53, 191, 770, and 4967) represented the majority (51.5%) of isolates. Fifty STs were associated with the pre-vaccination period (27 exclusive) and 43 (20 exclusive) with the post-vaccination period; 23 STs were identified in both periods. Some serotypes were particularly clonal (7F, 8, 12F, 20). Non-susceptibility to penicillin was associated with serotype 19A, CC320. Erythromycin resistance was heterogeneous when considering serotype and ST. A single serotype 23F (ST4967) isolate was resistant to levofloxacin. Continued surveillance is required to determine vaccine impact and to monitor changes in pneumococcal population biology post-PCV10 introduction in Brazil.

Show MeSH

Related in: MedlinePlus

Population structure of invasive S. pneumoniae collected from 2007 to 2012.Black circles: pre-vaccine isolates; Green circles: post-vaccine isolates; pink circles: isolates present in both pre and post vaccine years; Blue circles: the ancestor of the CC.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4214725&req=5

pone-0111129-g002: Population structure of invasive S. pneumoniae collected from 2007 to 2012.Black circles: pre-vaccine isolates; Green circles: post-vaccine isolates; pink circles: isolates present in both pre and post vaccine years; Blue circles: the ancestor of the CC.

Mentions: Two-hundred thirty-one pneumococci, belonging to the 33 most relevant and/or frequent serotypes, were characterized by MLST. Our population was composed of 70 different STs, with 9 CCs (composed of 25 STs) and 45 singletons (Table 3; Figure 2). Eight STs (156, 180, 218, 8889, 53, 191, 770 and 4967) represented 51.5% (119/231) of all isolates. Most serotypes were associated with more than one ST and a few STs were associated with more than one serotype (Table 3): ST72 (3 and 24F), ST156 (14, 9V and 9A), ST218 (12F and 7F), ST733 (16F, 19A and 19F), ST4967 (19A and 23F) and ST5406 (23F and 9A). Fifty STs were found in the pre-vaccination period (with 27 exclusively related to this period), while 43 STs (20 exclusive) were recovered from IPD after PCV10 implementation; 23 STs were identified in both periods (Table 4). Considering Simpson’s index of diversity (94.8% and 94.9%, for pre and post, respectively) and Shannon index (3.47 and 3.33 before and after, respectively), genetic diversity was similar in both periods (p = 0.996 and p = 0.348 for Simpson’s and Shannon indexes, respectively), which is also true for PCV10 (32 STs identified) versus non-PCV10 (43 STs characterized) serotypes (Simpson’s index: 89.44% vs 91.4%; p = 0.189).


Serotypes and genotypes of invasive Streptococcus pneumoniae before and after PCV10 implementation in southern Brazil.

Caierão J, Hawkins P, Sant'anna FH, da Cunha GR, d'Azevedo PA, McGee L, Dias C - PLoS ONE (2014)

Population structure of invasive S. pneumoniae collected from 2007 to 2012.Black circles: pre-vaccine isolates; Green circles: post-vaccine isolates; pink circles: isolates present in both pre and post vaccine years; Blue circles: the ancestor of the CC.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214725&req=5

pone-0111129-g002: Population structure of invasive S. pneumoniae collected from 2007 to 2012.Black circles: pre-vaccine isolates; Green circles: post-vaccine isolates; pink circles: isolates present in both pre and post vaccine years; Blue circles: the ancestor of the CC.
Mentions: Two-hundred thirty-one pneumococci, belonging to the 33 most relevant and/or frequent serotypes, were characterized by MLST. Our population was composed of 70 different STs, with 9 CCs (composed of 25 STs) and 45 singletons (Table 3; Figure 2). Eight STs (156, 180, 218, 8889, 53, 191, 770 and 4967) represented 51.5% (119/231) of all isolates. Most serotypes were associated with more than one ST and a few STs were associated with more than one serotype (Table 3): ST72 (3 and 24F), ST156 (14, 9V and 9A), ST218 (12F and 7F), ST733 (16F, 19A and 19F), ST4967 (19A and 23F) and ST5406 (23F and 9A). Fifty STs were found in the pre-vaccination period (with 27 exclusively related to this period), while 43 STs (20 exclusive) were recovered from IPD after PCV10 implementation; 23 STs were identified in both periods (Table 4). Considering Simpson’s index of diversity (94.8% and 94.9%, for pre and post, respectively) and Shannon index (3.47 and 3.33 before and after, respectively), genetic diversity was similar in both periods (p = 0.996 and p = 0.348 for Simpson’s and Shannon indexes, respectively), which is also true for PCV10 (32 STs identified) versus non-PCV10 (43 STs characterized) serotypes (Simpson’s index: 89.44% vs 91.4%; p = 0.189).

Bottom Line: To reduce the burden of pneumococcal diseases, different formulations of pneumococcal conjugate vaccines (PCV) have been introduced in many countries.Seventy different sequence types [STs] were found, accounting for 9 clonal complexes [CCs] and 45 singletons.Non-susceptibility to penicillin was associated with serotype 19A, CC320.

View Article: PubMed Central - PubMed

Affiliation: Federal University of Health Science of Porto Alegre, Rio Grande do Sul, Brazil.

ABSTRACT
To reduce the burden of pneumococcal diseases, different formulations of pneumococcal conjugate vaccines (PCV) have been introduced in many countries. In Brazil, PCV10 has been available since 2010. We aimed to analyze the serotype and genetic composition of invasive pneumococci from Brazil in pre- and post-vaccination periods (2007-2012). Antibiotic susceptibility was determined and genotypes of macrolide and fluoroquinolone resistance were characterized. The genotypes of isolates of the most frequent serotypes were determined by multilocus sequence typing. The study included 325 isolates, which were primarily recovered from blood. The most common serotypes recovered were 14, 3, 4, 23F, 7F, 9V, 12F, 20, 19F, 8, 19A, and 5. Thirty-eight pneumococci (11.7%) were from children ≤5 years old. Considering the overall population, PCV10 and PCV13 serotype coverage was 50.1% and 64.9%, respectively. During the pre-vaccine period, isolates with serotypes belonging to the PVC10 represented 51.5% (100/194), whereas in the post vaccine they represented 48.0% (63/131). PCV13 serotypes represented 67.5% (131/194) and 59.2% (77/131) of total for pre- and post-vaccination periods, respectively. Seventy different sequence types [STs] were found, accounting for 9 clonal complexes [CCs] and 45 singletons. Eight STs (156, 180, 218, 8889, 53, 191, 770, and 4967) represented the majority (51.5%) of isolates. Fifty STs were associated with the pre-vaccination period (27 exclusive) and 43 (20 exclusive) with the post-vaccination period; 23 STs were identified in both periods. Some serotypes were particularly clonal (7F, 8, 12F, 20). Non-susceptibility to penicillin was associated with serotype 19A, CC320. Erythromycin resistance was heterogeneous when considering serotype and ST. A single serotype 23F (ST4967) isolate was resistant to levofloxacin. Continued surveillance is required to determine vaccine impact and to monitor changes in pneumococcal population biology post-PCV10 introduction in Brazil.

Show MeSH
Related in: MedlinePlus