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Serotypes and genotypes of invasive Streptococcus pneumoniae before and after PCV10 implementation in southern Brazil.

Caierão J, Hawkins P, Sant'anna FH, da Cunha GR, d'Azevedo PA, McGee L, Dias C - PLoS ONE (2014)

Bottom Line: To reduce the burden of pneumococcal diseases, different formulations of pneumococcal conjugate vaccines (PCV) have been introduced in many countries.Seventy different sequence types [STs] were found, accounting for 9 clonal complexes [CCs] and 45 singletons.Non-susceptibility to penicillin was associated with serotype 19A, CC320.

View Article: PubMed Central - PubMed

Affiliation: Federal University of Health Science of Porto Alegre, Rio Grande do Sul, Brazil.

ABSTRACT
To reduce the burden of pneumococcal diseases, different formulations of pneumococcal conjugate vaccines (PCV) have been introduced in many countries. In Brazil, PCV10 has been available since 2010. We aimed to analyze the serotype and genetic composition of invasive pneumococci from Brazil in pre- and post-vaccination periods (2007-2012). Antibiotic susceptibility was determined and genotypes of macrolide and fluoroquinolone resistance were characterized. The genotypes of isolates of the most frequent serotypes were determined by multilocus sequence typing. The study included 325 isolates, which were primarily recovered from blood. The most common serotypes recovered were 14, 3, 4, 23F, 7F, 9V, 12F, 20, 19F, 8, 19A, and 5. Thirty-eight pneumococci (11.7%) were from children ≤5 years old. Considering the overall population, PCV10 and PCV13 serotype coverage was 50.1% and 64.9%, respectively. During the pre-vaccine period, isolates with serotypes belonging to the PVC10 represented 51.5% (100/194), whereas in the post vaccine they represented 48.0% (63/131). PCV13 serotypes represented 67.5% (131/194) and 59.2% (77/131) of total for pre- and post-vaccination periods, respectively. Seventy different sequence types [STs] were found, accounting for 9 clonal complexes [CCs] and 45 singletons. Eight STs (156, 180, 218, 8889, 53, 191, 770, and 4967) represented the majority (51.5%) of isolates. Fifty STs were associated with the pre-vaccination period (27 exclusive) and 43 (20 exclusive) with the post-vaccination period; 23 STs were identified in both periods. Some serotypes were particularly clonal (7F, 8, 12F, 20). Non-susceptibility to penicillin was associated with serotype 19A, CC320. Erythromycin resistance was heterogeneous when considering serotype and ST. A single serotype 23F (ST4967) isolate was resistant to levofloxacin. Continued surveillance is required to determine vaccine impact and to monitor changes in pneumococcal population biology post-PCV10 introduction in Brazil.

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Distribution of serotypes in pre- and post-vaccination period among pneumococci recovered from IPD, during 2007–2012.Black bars represent pre-PCV10 and gray bars post-PCV10.
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pone-0111129-g001: Distribution of serotypes in pre- and post-vaccination period among pneumococci recovered from IPD, during 2007–2012.Black bars represent pre-PCV10 and gray bars post-PCV10.

Mentions: Figure 1 shows the distribution of serotypes before and after PCV10 introduction. It was observed some increase or decrease in the frequency of specific serotypes (Table S1). However, none was statistically significant, not even in the vaccinated population (children less than 2 years old).


Serotypes and genotypes of invasive Streptococcus pneumoniae before and after PCV10 implementation in southern Brazil.

Caierão J, Hawkins P, Sant'anna FH, da Cunha GR, d'Azevedo PA, McGee L, Dias C - PLoS ONE (2014)

Distribution of serotypes in pre- and post-vaccination period among pneumococci recovered from IPD, during 2007–2012.Black bars represent pre-PCV10 and gray bars post-PCV10.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214725&req=5

pone-0111129-g001: Distribution of serotypes in pre- and post-vaccination period among pneumococci recovered from IPD, during 2007–2012.Black bars represent pre-PCV10 and gray bars post-PCV10.
Mentions: Figure 1 shows the distribution of serotypes before and after PCV10 introduction. It was observed some increase or decrease in the frequency of specific serotypes (Table S1). However, none was statistically significant, not even in the vaccinated population (children less than 2 years old).

Bottom Line: To reduce the burden of pneumococcal diseases, different formulations of pneumococcal conjugate vaccines (PCV) have been introduced in many countries.Seventy different sequence types [STs] were found, accounting for 9 clonal complexes [CCs] and 45 singletons.Non-susceptibility to penicillin was associated with serotype 19A, CC320.

View Article: PubMed Central - PubMed

Affiliation: Federal University of Health Science of Porto Alegre, Rio Grande do Sul, Brazil.

ABSTRACT
To reduce the burden of pneumococcal diseases, different formulations of pneumococcal conjugate vaccines (PCV) have been introduced in many countries. In Brazil, PCV10 has been available since 2010. We aimed to analyze the serotype and genetic composition of invasive pneumococci from Brazil in pre- and post-vaccination periods (2007-2012). Antibiotic susceptibility was determined and genotypes of macrolide and fluoroquinolone resistance were characterized. The genotypes of isolates of the most frequent serotypes were determined by multilocus sequence typing. The study included 325 isolates, which were primarily recovered from blood. The most common serotypes recovered were 14, 3, 4, 23F, 7F, 9V, 12F, 20, 19F, 8, 19A, and 5. Thirty-eight pneumococci (11.7%) were from children ≤5 years old. Considering the overall population, PCV10 and PCV13 serotype coverage was 50.1% and 64.9%, respectively. During the pre-vaccine period, isolates with serotypes belonging to the PVC10 represented 51.5% (100/194), whereas in the post vaccine they represented 48.0% (63/131). PCV13 serotypes represented 67.5% (131/194) and 59.2% (77/131) of total for pre- and post-vaccination periods, respectively. Seventy different sequence types [STs] were found, accounting for 9 clonal complexes [CCs] and 45 singletons. Eight STs (156, 180, 218, 8889, 53, 191, 770, and 4967) represented the majority (51.5%) of isolates. Fifty STs were associated with the pre-vaccination period (27 exclusive) and 43 (20 exclusive) with the post-vaccination period; 23 STs were identified in both periods. Some serotypes were particularly clonal (7F, 8, 12F, 20). Non-susceptibility to penicillin was associated with serotype 19A, CC320. Erythromycin resistance was heterogeneous when considering serotype and ST. A single serotype 23F (ST4967) isolate was resistant to levofloxacin. Continued surveillance is required to determine vaccine impact and to monitor changes in pneumococcal population biology post-PCV10 introduction in Brazil.

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