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Comparative endothelial cell response on topographically patterned titanium and silicon substrates with micrometer to sub-micrometer feature sizes.

Vandrangi P, Gott SC, Kozaka R, Rodgers VG, Rao MP - PLoS ONE (2014)

Bottom Line: These specific materials are chosen due to their relevance for implantable microdevice applications, while grating-based patterns are chosen for the potential they afford for inducing elongated and aligned cellular morphologies reminiscent of the native endothelium.Moreover, we show similar trending on patterned Si substrates, albeit to a lesser extent than on comparably patterned Ti substrates.Collectively, these results suggest promise for sub-micrometer topographic patterning in general, and sub-micrometer patterning of Ti specifically, as a means for enhancing endothelialization and neovascularisation for novel implantable microdevice applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Engineering, University of California Riverside, Riverside, California, United States of America; Department of Bioengineering, University of California Riverside, Riverside, California, United States of America.

ABSTRACT
In this work, we evaluate the in vitro response of endothelial cells (EC) to variation in precisely-defined, micrometer to sub-micrometer scale topography on two different substrate materials, titanium (Ti) and silicon (Si). Both substrates possess identically-patterned surfaces composed of microfabricated, groove-based gratings with groove widths ranging from 0.5 to 50 µm, grating pitch twice the groove width, and groove depth of 1.3 µm. These specific materials are chosen due to their relevance for implantable microdevice applications, while grating-based patterns are chosen for the potential they afford for inducing elongated and aligned cellular morphologies reminiscent of the native endothelium. Using EA926 cells, a human EC variant, we show significant improvement in cellular adhesion, proliferation, morphology, and function with decreasing feature size on patterned Ti substrates. Moreover, we show similar trending on patterned Si substrates, albeit to a lesser extent than on comparably patterned Ti substrates. Collectively, these results suggest promise for sub-micrometer topographic patterning in general, and sub-micrometer patterning of Ti specifically, as a means for enhancing endothelialization and neovascularisation for novel implantable microdevice applications.

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Confocal micrographs and quantification of human endothelial cell protein expression (red) after 1 day culture on 0.5 µm gratings and unpatterned sub-patterns of Ti and Si substrates, and tissue culture plastic: A) vWF; and B) VCAM-1.Nuclei (blue) were cross-stained using Hoechst 33342. Double arrows indicate grating direction. Data = mean ± SEM (**p = 0.01, ***p = 0.001; unpaired samples T-test, n = 10).
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pone-0111465-g010: Confocal micrographs and quantification of human endothelial cell protein expression (red) after 1 day culture on 0.5 µm gratings and unpatterned sub-patterns of Ti and Si substrates, and tissue culture plastic: A) vWF; and B) VCAM-1.Nuclei (blue) were cross-stained using Hoechst 33342. Double arrows indicate grating direction. Data = mean ± SEM (**p = 0.01, ***p = 0.001; unpaired samples T-test, n = 10).

Mentions: Figure 10 shows results for expression of two important EC markers, vWF and VCAM-1, on the patterned Ti and Si substrates. As discussed earlier, vWF is a functional marker expressed by ECs that plays a key role in homeostasis, whereas VCAM-1 is a marker for inflammation.


Comparative endothelial cell response on topographically patterned titanium and silicon substrates with micrometer to sub-micrometer feature sizes.

Vandrangi P, Gott SC, Kozaka R, Rodgers VG, Rao MP - PLoS ONE (2014)

Confocal micrographs and quantification of human endothelial cell protein expression (red) after 1 day culture on 0.5 µm gratings and unpatterned sub-patterns of Ti and Si substrates, and tissue culture plastic: A) vWF; and B) VCAM-1.Nuclei (blue) were cross-stained using Hoechst 33342. Double arrows indicate grating direction. Data = mean ± SEM (**p = 0.01, ***p = 0.001; unpaired samples T-test, n = 10).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214724&req=5

pone-0111465-g010: Confocal micrographs and quantification of human endothelial cell protein expression (red) after 1 day culture on 0.5 µm gratings and unpatterned sub-patterns of Ti and Si substrates, and tissue culture plastic: A) vWF; and B) VCAM-1.Nuclei (blue) were cross-stained using Hoechst 33342. Double arrows indicate grating direction. Data = mean ± SEM (**p = 0.01, ***p = 0.001; unpaired samples T-test, n = 10).
Mentions: Figure 10 shows results for expression of two important EC markers, vWF and VCAM-1, on the patterned Ti and Si substrates. As discussed earlier, vWF is a functional marker expressed by ECs that plays a key role in homeostasis, whereas VCAM-1 is a marker for inflammation.

Bottom Line: These specific materials are chosen due to their relevance for implantable microdevice applications, while grating-based patterns are chosen for the potential they afford for inducing elongated and aligned cellular morphologies reminiscent of the native endothelium.Moreover, we show similar trending on patterned Si substrates, albeit to a lesser extent than on comparably patterned Ti substrates.Collectively, these results suggest promise for sub-micrometer topographic patterning in general, and sub-micrometer patterning of Ti specifically, as a means for enhancing endothelialization and neovascularisation for novel implantable microdevice applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Engineering, University of California Riverside, Riverside, California, United States of America; Department of Bioengineering, University of California Riverside, Riverside, California, United States of America.

ABSTRACT
In this work, we evaluate the in vitro response of endothelial cells (EC) to variation in precisely-defined, micrometer to sub-micrometer scale topography on two different substrate materials, titanium (Ti) and silicon (Si). Both substrates possess identically-patterned surfaces composed of microfabricated, groove-based gratings with groove widths ranging from 0.5 to 50 µm, grating pitch twice the groove width, and groove depth of 1.3 µm. These specific materials are chosen due to their relevance for implantable microdevice applications, while grating-based patterns are chosen for the potential they afford for inducing elongated and aligned cellular morphologies reminiscent of the native endothelium. Using EA926 cells, a human EC variant, we show significant improvement in cellular adhesion, proliferation, morphology, and function with decreasing feature size on patterned Ti substrates. Moreover, we show similar trending on patterned Si substrates, albeit to a lesser extent than on comparably patterned Ti substrates. Collectively, these results suggest promise for sub-micrometer topographic patterning in general, and sub-micrometer patterning of Ti specifically, as a means for enhancing endothelialization and neovascularisation for novel implantable microdevice applications.

Show MeSH
Related in: MedlinePlus