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Comparative endothelial cell response on topographically patterned titanium and silicon substrates with micrometer to sub-micrometer feature sizes.

Vandrangi P, Gott SC, Kozaka R, Rodgers VG, Rao MP - PLoS ONE (2014)

Bottom Line: These specific materials are chosen due to their relevance for implantable microdevice applications, while grating-based patterns are chosen for the potential they afford for inducing elongated and aligned cellular morphologies reminiscent of the native endothelium.Moreover, we show similar trending on patterned Si substrates, albeit to a lesser extent than on comparably patterned Ti substrates.Collectively, these results suggest promise for sub-micrometer topographic patterning in general, and sub-micrometer patterning of Ti specifically, as a means for enhancing endothelialization and neovascularisation for novel implantable microdevice applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Engineering, University of California Riverside, Riverside, California, United States of America; Department of Bioengineering, University of California Riverside, Riverside, California, United States of America.

ABSTRACT
In this work, we evaluate the in vitro response of endothelial cells (EC) to variation in precisely-defined, micrometer to sub-micrometer scale topography on two different substrate materials, titanium (Ti) and silicon (Si). Both substrates possess identically-patterned surfaces composed of microfabricated, groove-based gratings with groove widths ranging from 0.5 to 50 µm, grating pitch twice the groove width, and groove depth of 1.3 µm. These specific materials are chosen due to their relevance for implantable microdevice applications, while grating-based patterns are chosen for the potential they afford for inducing elongated and aligned cellular morphologies reminiscent of the native endothelium. Using EA926 cells, a human EC variant, we show significant improvement in cellular adhesion, proliferation, morphology, and function with decreasing feature size on patterned Ti substrates. Moreover, we show similar trending on patterned Si substrates, albeit to a lesser extent than on comparably patterned Ti substrates. Collectively, these results suggest promise for sub-micrometer topographic patterning in general, and sub-micrometer patterning of Ti specifically, as a means for enhancing endothelialization and neovascularisation for novel implantable microdevice applications.

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Confocal micrographs of human endothelial cells after 5 day culture on 0.5 µm gratings and unpatterned sub-patterns of Ti (left) and Si (right) substrates.Cells were immunostained using phalloidin (red) for cytoskeletal protein F-actin, and Hoechst 33342 (blue) for nuclei. Double arrows indicate grating direction.
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pone-0111465-g009: Confocal micrographs of human endothelial cells after 5 day culture on 0.5 µm gratings and unpatterned sub-patterns of Ti (left) and Si (right) substrates.Cells were immunostained using phalloidin (red) for cytoskeletal protein F-actin, and Hoechst 33342 (blue) for nuclei. Double arrows indicate grating direction.

Mentions: The influence of sub-micrometer patterning and substrate material on cytoskeletal architecture is illustrated in Figure 9, which shows HECs stained for nuclei and F-actin on 0.5 µm gratings and unpatterned sub-patterns of both substrate materials after 5 d. Strong cytoskeletal alignment is evident on the gratings of both materials, which is corroborated by the increasing cellular elongation ratios and decreasing angular deviations with decreasing feature size reported in Table 3. However, the microfilament network on the 0.5 µm Ti grating is observed to be more robust relative to the 0.5 µm Si grating, and greater elongation is seen on the Ti grating. Cytoskeletal alignment is not observed on the unpatterned controls for either substrate. This is further corroborated by the measured ∼45° mean angular deviation on the unpatterned controls for either substrate, which is indicative of randomized cell orientation.


Comparative endothelial cell response on topographically patterned titanium and silicon substrates with micrometer to sub-micrometer feature sizes.

Vandrangi P, Gott SC, Kozaka R, Rodgers VG, Rao MP - PLoS ONE (2014)

Confocal micrographs of human endothelial cells after 5 day culture on 0.5 µm gratings and unpatterned sub-patterns of Ti (left) and Si (right) substrates.Cells were immunostained using phalloidin (red) for cytoskeletal protein F-actin, and Hoechst 33342 (blue) for nuclei. Double arrows indicate grating direction.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214724&req=5

pone-0111465-g009: Confocal micrographs of human endothelial cells after 5 day culture on 0.5 µm gratings and unpatterned sub-patterns of Ti (left) and Si (right) substrates.Cells were immunostained using phalloidin (red) for cytoskeletal protein F-actin, and Hoechst 33342 (blue) for nuclei. Double arrows indicate grating direction.
Mentions: The influence of sub-micrometer patterning and substrate material on cytoskeletal architecture is illustrated in Figure 9, which shows HECs stained for nuclei and F-actin on 0.5 µm gratings and unpatterned sub-patterns of both substrate materials after 5 d. Strong cytoskeletal alignment is evident on the gratings of both materials, which is corroborated by the increasing cellular elongation ratios and decreasing angular deviations with decreasing feature size reported in Table 3. However, the microfilament network on the 0.5 µm Ti grating is observed to be more robust relative to the 0.5 µm Si grating, and greater elongation is seen on the Ti grating. Cytoskeletal alignment is not observed on the unpatterned controls for either substrate. This is further corroborated by the measured ∼45° mean angular deviation on the unpatterned controls for either substrate, which is indicative of randomized cell orientation.

Bottom Line: These specific materials are chosen due to their relevance for implantable microdevice applications, while grating-based patterns are chosen for the potential they afford for inducing elongated and aligned cellular morphologies reminiscent of the native endothelium.Moreover, we show similar trending on patterned Si substrates, albeit to a lesser extent than on comparably patterned Ti substrates.Collectively, these results suggest promise for sub-micrometer topographic patterning in general, and sub-micrometer patterning of Ti specifically, as a means for enhancing endothelialization and neovascularisation for novel implantable microdevice applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Engineering, University of California Riverside, Riverside, California, United States of America; Department of Bioengineering, University of California Riverside, Riverside, California, United States of America.

ABSTRACT
In this work, we evaluate the in vitro response of endothelial cells (EC) to variation in precisely-defined, micrometer to sub-micrometer scale topography on two different substrate materials, titanium (Ti) and silicon (Si). Both substrates possess identically-patterned surfaces composed of microfabricated, groove-based gratings with groove widths ranging from 0.5 to 50 µm, grating pitch twice the groove width, and groove depth of 1.3 µm. These specific materials are chosen due to their relevance for implantable microdevice applications, while grating-based patterns are chosen for the potential they afford for inducing elongated and aligned cellular morphologies reminiscent of the native endothelium. Using EA926 cells, a human EC variant, we show significant improvement in cellular adhesion, proliferation, morphology, and function with decreasing feature size on patterned Ti substrates. Moreover, we show similar trending on patterned Si substrates, albeit to a lesser extent than on comparably patterned Ti substrates. Collectively, these results suggest promise for sub-micrometer topographic patterning in general, and sub-micrometer patterning of Ti specifically, as a means for enhancing endothelialization and neovascularisation for novel implantable microdevice applications.

Show MeSH
Related in: MedlinePlus