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Comparative endothelial cell response on topographically patterned titanium and silicon substrates with micrometer to sub-micrometer feature sizes.

Vandrangi P, Gott SC, Kozaka R, Rodgers VG, Rao MP - PLoS ONE (2014)

Bottom Line: These specific materials are chosen due to their relevance for implantable microdevice applications, while grating-based patterns are chosen for the potential they afford for inducing elongated and aligned cellular morphologies reminiscent of the native endothelium.Moreover, we show similar trending on patterned Si substrates, albeit to a lesser extent than on comparably patterned Ti substrates.Collectively, these results suggest promise for sub-micrometer topographic patterning in general, and sub-micrometer patterning of Ti specifically, as a means for enhancing endothelialization and neovascularisation for novel implantable microdevice applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Engineering, University of California Riverside, Riverside, California, United States of America; Department of Bioengineering, University of California Riverside, Riverside, California, United States of America.

ABSTRACT
In this work, we evaluate the in vitro response of endothelial cells (EC) to variation in precisely-defined, micrometer to sub-micrometer scale topography on two different substrate materials, titanium (Ti) and silicon (Si). Both substrates possess identically-patterned surfaces composed of microfabricated, groove-based gratings with groove widths ranging from 0.5 to 50 µm, grating pitch twice the groove width, and groove depth of 1.3 µm. These specific materials are chosen due to their relevance for implantable microdevice applications, while grating-based patterns are chosen for the potential they afford for inducing elongated and aligned cellular morphologies reminiscent of the native endothelium. Using EA926 cells, a human EC variant, we show significant improvement in cellular adhesion, proliferation, morphology, and function with decreasing feature size on patterned Ti substrates. Moreover, we show similar trending on patterned Si substrates, albeit to a lesser extent than on comparably patterned Ti substrates. Collectively, these results suggest promise for sub-micrometer topographic patterning in general, and sub-micrometer patterning of Ti specifically, as a means for enhancing endothelialization and neovascularisation for novel implantable microdevice applications.

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Scanning electron microscope micrographs of human endothelial cells after 5 day culture on patterned Ti (left) and Si (right) substrates.Each micrograph depicts a single field of view located at the boundary between the 0.5 µm and 50 µm grating sub-patterns, as illustrated in the schematics below each micrograph. Dotted lines indicate boundaries of the sub-patterns and double arrows indicate grating directions.
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pone-0111465-g007: Scanning electron microscope micrographs of human endothelial cells after 5 day culture on patterned Ti (left) and Si (right) substrates.Each micrograph depicts a single field of view located at the boundary between the 0.5 µm and 50 µm grating sub-patterns, as illustrated in the schematics below each micrograph. Dotted lines indicate boundaries of the sub-patterns and double arrows indicate grating directions.

Mentions: Similar trends are observed at latter time points, as illustrated in Figure 7, which shows SEM micrographs of HECs on patterned Ti and Si substrates after 5 d. These images were taken at the boundary between the 0.5 µm and 50 µm sub-patterns, which are separated by a 200 µm wide unpatterned region, and are orthogonally oriented with respect to one another. Cellular elongation and alignment are again seen on the 0.5 µm gratings of both materials, with more favorable morphology and greater coverage on the 0.5 µm Ti grating. Moreover, the Ti micrograph clearly illustrates the spatial specificity of HEC response to the 0.5 µm Ti grating, as evidenced by the decreasing cell alignment and density with distance from the boundary of the 0.5 µm sub-pattern.


Comparative endothelial cell response on topographically patterned titanium and silicon substrates with micrometer to sub-micrometer feature sizes.

Vandrangi P, Gott SC, Kozaka R, Rodgers VG, Rao MP - PLoS ONE (2014)

Scanning electron microscope micrographs of human endothelial cells after 5 day culture on patterned Ti (left) and Si (right) substrates.Each micrograph depicts a single field of view located at the boundary between the 0.5 µm and 50 µm grating sub-patterns, as illustrated in the schematics below each micrograph. Dotted lines indicate boundaries of the sub-patterns and double arrows indicate grating directions.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214724&req=5

pone-0111465-g007: Scanning electron microscope micrographs of human endothelial cells after 5 day culture on patterned Ti (left) and Si (right) substrates.Each micrograph depicts a single field of view located at the boundary between the 0.5 µm and 50 µm grating sub-patterns, as illustrated in the schematics below each micrograph. Dotted lines indicate boundaries of the sub-patterns and double arrows indicate grating directions.
Mentions: Similar trends are observed at latter time points, as illustrated in Figure 7, which shows SEM micrographs of HECs on patterned Ti and Si substrates after 5 d. These images were taken at the boundary between the 0.5 µm and 50 µm sub-patterns, which are separated by a 200 µm wide unpatterned region, and are orthogonally oriented with respect to one another. Cellular elongation and alignment are again seen on the 0.5 µm gratings of both materials, with more favorable morphology and greater coverage on the 0.5 µm Ti grating. Moreover, the Ti micrograph clearly illustrates the spatial specificity of HEC response to the 0.5 µm Ti grating, as evidenced by the decreasing cell alignment and density with distance from the boundary of the 0.5 µm sub-pattern.

Bottom Line: These specific materials are chosen due to their relevance for implantable microdevice applications, while grating-based patterns are chosen for the potential they afford for inducing elongated and aligned cellular morphologies reminiscent of the native endothelium.Moreover, we show similar trending on patterned Si substrates, albeit to a lesser extent than on comparably patterned Ti substrates.Collectively, these results suggest promise for sub-micrometer topographic patterning in general, and sub-micrometer patterning of Ti specifically, as a means for enhancing endothelialization and neovascularisation for novel implantable microdevice applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Engineering, University of California Riverside, Riverside, California, United States of America; Department of Bioengineering, University of California Riverside, Riverside, California, United States of America.

ABSTRACT
In this work, we evaluate the in vitro response of endothelial cells (EC) to variation in precisely-defined, micrometer to sub-micrometer scale topography on two different substrate materials, titanium (Ti) and silicon (Si). Both substrates possess identically-patterned surfaces composed of microfabricated, groove-based gratings with groove widths ranging from 0.5 to 50 µm, grating pitch twice the groove width, and groove depth of 1.3 µm. These specific materials are chosen due to their relevance for implantable microdevice applications, while grating-based patterns are chosen for the potential they afford for inducing elongated and aligned cellular morphologies reminiscent of the native endothelium. Using EA926 cells, a human EC variant, we show significant improvement in cellular adhesion, proliferation, morphology, and function with decreasing feature size on patterned Ti substrates. Moreover, we show similar trending on patterned Si substrates, albeit to a lesser extent than on comparably patterned Ti substrates. Collectively, these results suggest promise for sub-micrometer topographic patterning in general, and sub-micrometer patterning of Ti specifically, as a means for enhancing endothelialization and neovascularisation for novel implantable microdevice applications.

Show MeSH
Related in: MedlinePlus