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Comparative endothelial cell response on topographically patterned titanium and silicon substrates with micrometer to sub-micrometer feature sizes.

Vandrangi P, Gott SC, Kozaka R, Rodgers VG, Rao MP - PLoS ONE (2014)

Bottom Line: These specific materials are chosen due to their relevance for implantable microdevice applications, while grating-based patterns are chosen for the potential they afford for inducing elongated and aligned cellular morphologies reminiscent of the native endothelium.Moreover, we show similar trending on patterned Si substrates, albeit to a lesser extent than on comparably patterned Ti substrates.Collectively, these results suggest promise for sub-micrometer topographic patterning in general, and sub-micrometer patterning of Ti specifically, as a means for enhancing endothelialization and neovascularisation for novel implantable microdevice applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Engineering, University of California Riverside, Riverside, California, United States of America; Department of Bioengineering, University of California Riverside, Riverside, California, United States of America.

ABSTRACT
In this work, we evaluate the in vitro response of endothelial cells (EC) to variation in precisely-defined, micrometer to sub-micrometer scale topography on two different substrate materials, titanium (Ti) and silicon (Si). Both substrates possess identically-patterned surfaces composed of microfabricated, groove-based gratings with groove widths ranging from 0.5 to 50 µm, grating pitch twice the groove width, and groove depth of 1.3 µm. These specific materials are chosen due to their relevance for implantable microdevice applications, while grating-based patterns are chosen for the potential they afford for inducing elongated and aligned cellular morphologies reminiscent of the native endothelium. Using EA926 cells, a human EC variant, we show significant improvement in cellular adhesion, proliferation, morphology, and function with decreasing feature size on patterned Ti substrates. Moreover, we show similar trending on patterned Si substrates, albeit to a lesser extent than on comparably patterned Ti substrates. Collectively, these results suggest promise for sub-micrometer topographic patterning in general, and sub-micrometer patterning of Ti specifically, as a means for enhancing endothelialization and neovascularisation for novel implantable microdevice applications.

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Scanning electron microscope micrographs of human endothelial cells after 1 day culture on 0.5 µm gratings and unpatterned sub-patterns of Ti (left) and Si (right) substrates.Arrows indicate filopodia and lamellopodia.
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pone-0111465-g006: Scanning electron microscope micrographs of human endothelial cells after 1 day culture on 0.5 µm gratings and unpatterned sub-patterns of Ti (left) and Si (right) substrates.Arrows indicate filopodia and lamellopodia.

Mentions: Figure 6 shows SEM micrographs of HECs on 0.5 µm gratings and unpatterned sub-patterns of both substrate materials after 1 d. Significant cellular elongation is observed on the Ti grating and alignment along the grating axis is clear. For the Si grating, significant cellular elongation is also seen, as is alignment with the grating axis. However, more favorable cellular morphology occurs on the Ti grating relative to the Si grating, as evidenced by greater flattening and spreading. Greater spreading is also seen on the unpatterned Ti relative to unpatterned Si surfaces.


Comparative endothelial cell response on topographically patterned titanium and silicon substrates with micrometer to sub-micrometer feature sizes.

Vandrangi P, Gott SC, Kozaka R, Rodgers VG, Rao MP - PLoS ONE (2014)

Scanning electron microscope micrographs of human endothelial cells after 1 day culture on 0.5 µm gratings and unpatterned sub-patterns of Ti (left) and Si (right) substrates.Arrows indicate filopodia and lamellopodia.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214724&req=5

pone-0111465-g006: Scanning electron microscope micrographs of human endothelial cells after 1 day culture on 0.5 µm gratings and unpatterned sub-patterns of Ti (left) and Si (right) substrates.Arrows indicate filopodia and lamellopodia.
Mentions: Figure 6 shows SEM micrographs of HECs on 0.5 µm gratings and unpatterned sub-patterns of both substrate materials after 1 d. Significant cellular elongation is observed on the Ti grating and alignment along the grating axis is clear. For the Si grating, significant cellular elongation is also seen, as is alignment with the grating axis. However, more favorable cellular morphology occurs on the Ti grating relative to the Si grating, as evidenced by greater flattening and spreading. Greater spreading is also seen on the unpatterned Ti relative to unpatterned Si surfaces.

Bottom Line: These specific materials are chosen due to their relevance for implantable microdevice applications, while grating-based patterns are chosen for the potential they afford for inducing elongated and aligned cellular morphologies reminiscent of the native endothelium.Moreover, we show similar trending on patterned Si substrates, albeit to a lesser extent than on comparably patterned Ti substrates.Collectively, these results suggest promise for sub-micrometer topographic patterning in general, and sub-micrometer patterning of Ti specifically, as a means for enhancing endothelialization and neovascularisation for novel implantable microdevice applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Engineering, University of California Riverside, Riverside, California, United States of America; Department of Bioengineering, University of California Riverside, Riverside, California, United States of America.

ABSTRACT
In this work, we evaluate the in vitro response of endothelial cells (EC) to variation in precisely-defined, micrometer to sub-micrometer scale topography on two different substrate materials, titanium (Ti) and silicon (Si). Both substrates possess identically-patterned surfaces composed of microfabricated, groove-based gratings with groove widths ranging from 0.5 to 50 µm, grating pitch twice the groove width, and groove depth of 1.3 µm. These specific materials are chosen due to their relevance for implantable microdevice applications, while grating-based patterns are chosen for the potential they afford for inducing elongated and aligned cellular morphologies reminiscent of the native endothelium. Using EA926 cells, a human EC variant, we show significant improvement in cellular adhesion, proliferation, morphology, and function with decreasing feature size on patterned Ti substrates. Moreover, we show similar trending on patterned Si substrates, albeit to a lesser extent than on comparably patterned Ti substrates. Collectively, these results suggest promise for sub-micrometer topographic patterning in general, and sub-micrometer patterning of Ti specifically, as a means for enhancing endothelialization and neovascularisation for novel implantable microdevice applications.

Show MeSH
Related in: MedlinePlus