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Comparative endothelial cell response on topographically patterned titanium and silicon substrates with micrometer to sub-micrometer feature sizes.

Vandrangi P, Gott SC, Kozaka R, Rodgers VG, Rao MP - PLoS ONE (2014)

Bottom Line: These specific materials are chosen due to their relevance for implantable microdevice applications, while grating-based patterns are chosen for the potential they afford for inducing elongated and aligned cellular morphologies reminiscent of the native endothelium.Moreover, we show similar trending on patterned Si substrates, albeit to a lesser extent than on comparably patterned Ti substrates.Collectively, these results suggest promise for sub-micrometer topographic patterning in general, and sub-micrometer patterning of Ti specifically, as a means for enhancing endothelialization and neovascularisation for novel implantable microdevice applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Engineering, University of California Riverside, Riverside, California, United States of America; Department of Bioengineering, University of California Riverside, Riverside, California, United States of America.

ABSTRACT
In this work, we evaluate the in vitro response of endothelial cells (EC) to variation in precisely-defined, micrometer to sub-micrometer scale topography on two different substrate materials, titanium (Ti) and silicon (Si). Both substrates possess identically-patterned surfaces composed of microfabricated, groove-based gratings with groove widths ranging from 0.5 to 50 µm, grating pitch twice the groove width, and groove depth of 1.3 µm. These specific materials are chosen due to their relevance for implantable microdevice applications, while grating-based patterns are chosen for the potential they afford for inducing elongated and aligned cellular morphologies reminiscent of the native endothelium. Using EA926 cells, a human EC variant, we show significant improvement in cellular adhesion, proliferation, morphology, and function with decreasing feature size on patterned Ti substrates. Moreover, we show similar trending on patterned Si substrates, albeit to a lesser extent than on comparably patterned Ti substrates. Collectively, these results suggest promise for sub-micrometer topographic patterning in general, and sub-micrometer patterning of Ti specifically, as a means for enhancing endothelialization and neovascularisation for novel implantable microdevice applications.

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Human endothelial cell densities on patterned Ti and Si substrates and tissue culture plastic at varying time points ranging from 30 min (i.e. 0 days) to 5 days.Data = mean ± SEM (**p = 0.01, ****p = 0.0001; unpaired samples T-test, n = 5).
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pone-0111465-g005: Human endothelial cell densities on patterned Ti and Si substrates and tissue culture plastic at varying time points ranging from 30 min (i.e. 0 days) to 5 days.Data = mean ± SEM (**p = 0.01, ****p = 0.0001; unpaired samples T-test, n = 5).

Mentions: Figure 5 shows HEC densities at various time points on the patterned Ti and Si substrates. At the 0 d time point (i.e. 30 min), we observe a trend of increasing adhesion with decreasing feature size on the patterned Ti substrates, and response on the patterned Ti surfaces is greater than the unpatterned Ti control, e.g., HEC densities on the 0.5 µm Ti gratings are 2.32 times greater than unpatterned Ti. For the patterned Si substrates, similar size-dependent response is observed, e.g., HEC densities on the 0.5 µm Si gratings are 2 times greater than unpatterned Si. However, adhesion on patterned Si is generally lower than on comparably patterned Ti, e.g., HEC densities on the 0.5 µm Si gratings are 14% lower than on 0.5 µm Ti gratings. Finally, adhesion on both patterned and unpatterned Ti and Si is greater than the tissue culture plastic control.


Comparative endothelial cell response on topographically patterned titanium and silicon substrates with micrometer to sub-micrometer feature sizes.

Vandrangi P, Gott SC, Kozaka R, Rodgers VG, Rao MP - PLoS ONE (2014)

Human endothelial cell densities on patterned Ti and Si substrates and tissue culture plastic at varying time points ranging from 30 min (i.e. 0 days) to 5 days.Data = mean ± SEM (**p = 0.01, ****p = 0.0001; unpaired samples T-test, n = 5).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214724&req=5

pone-0111465-g005: Human endothelial cell densities on patterned Ti and Si substrates and tissue culture plastic at varying time points ranging from 30 min (i.e. 0 days) to 5 days.Data = mean ± SEM (**p = 0.01, ****p = 0.0001; unpaired samples T-test, n = 5).
Mentions: Figure 5 shows HEC densities at various time points on the patterned Ti and Si substrates. At the 0 d time point (i.e. 30 min), we observe a trend of increasing adhesion with decreasing feature size on the patterned Ti substrates, and response on the patterned Ti surfaces is greater than the unpatterned Ti control, e.g., HEC densities on the 0.5 µm Ti gratings are 2.32 times greater than unpatterned Ti. For the patterned Si substrates, similar size-dependent response is observed, e.g., HEC densities on the 0.5 µm Si gratings are 2 times greater than unpatterned Si. However, adhesion on patterned Si is generally lower than on comparably patterned Ti, e.g., HEC densities on the 0.5 µm Si gratings are 14% lower than on 0.5 µm Ti gratings. Finally, adhesion on both patterned and unpatterned Ti and Si is greater than the tissue culture plastic control.

Bottom Line: These specific materials are chosen due to their relevance for implantable microdevice applications, while grating-based patterns are chosen for the potential they afford for inducing elongated and aligned cellular morphologies reminiscent of the native endothelium.Moreover, we show similar trending on patterned Si substrates, albeit to a lesser extent than on comparably patterned Ti substrates.Collectively, these results suggest promise for sub-micrometer topographic patterning in general, and sub-micrometer patterning of Ti specifically, as a means for enhancing endothelialization and neovascularisation for novel implantable microdevice applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Engineering, University of California Riverside, Riverside, California, United States of America; Department of Bioengineering, University of California Riverside, Riverside, California, United States of America.

ABSTRACT
In this work, we evaluate the in vitro response of endothelial cells (EC) to variation in precisely-defined, micrometer to sub-micrometer scale topography on two different substrate materials, titanium (Ti) and silicon (Si). Both substrates possess identically-patterned surfaces composed of microfabricated, groove-based gratings with groove widths ranging from 0.5 to 50 µm, grating pitch twice the groove width, and groove depth of 1.3 µm. These specific materials are chosen due to their relevance for implantable microdevice applications, while grating-based patterns are chosen for the potential they afford for inducing elongated and aligned cellular morphologies reminiscent of the native endothelium. Using EA926 cells, a human EC variant, we show significant improvement in cellular adhesion, proliferation, morphology, and function with decreasing feature size on patterned Ti substrates. Moreover, we show similar trending on patterned Si substrates, albeit to a lesser extent than on comparably patterned Ti substrates. Collectively, these results suggest promise for sub-micrometer topographic patterning in general, and sub-micrometer patterning of Ti specifically, as a means for enhancing endothelialization and neovascularisation for novel implantable microdevice applications.

Show MeSH
Related in: MedlinePlus