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Comparative endothelial cell response on topographically patterned titanium and silicon substrates with micrometer to sub-micrometer feature sizes.

Vandrangi P, Gott SC, Kozaka R, Rodgers VG, Rao MP - PLoS ONE (2014)

Bottom Line: These specific materials are chosen due to their relevance for implantable microdevice applications, while grating-based patterns are chosen for the potential they afford for inducing elongated and aligned cellular morphologies reminiscent of the native endothelium.Moreover, we show similar trending on patterned Si substrates, albeit to a lesser extent than on comparably patterned Ti substrates.Collectively, these results suggest promise for sub-micrometer topographic patterning in general, and sub-micrometer patterning of Ti specifically, as a means for enhancing endothelialization and neovascularisation for novel implantable microdevice applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Engineering, University of California Riverside, Riverside, California, United States of America; Department of Bioengineering, University of California Riverside, Riverside, California, United States of America.

ABSTRACT
In this work, we evaluate the in vitro response of endothelial cells (EC) to variation in precisely-defined, micrometer to sub-micrometer scale topography on two different substrate materials, titanium (Ti) and silicon (Si). Both substrates possess identically-patterned surfaces composed of microfabricated, groove-based gratings with groove widths ranging from 0.5 to 50 µm, grating pitch twice the groove width, and groove depth of 1.3 µm. These specific materials are chosen due to their relevance for implantable microdevice applications, while grating-based patterns are chosen for the potential they afford for inducing elongated and aligned cellular morphologies reminiscent of the native endothelium. Using EA926 cells, a human EC variant, we show significant improvement in cellular adhesion, proliferation, morphology, and function with decreasing feature size on patterned Ti substrates. Moreover, we show similar trending on patterned Si substrates, albeit to a lesser extent than on comparably patterned Ti substrates. Collectively, these results suggest promise for sub-micrometer topographic patterning in general, and sub-micrometer patterning of Ti specifically, as a means for enhancing endothelialization and neovascularisation for novel implantable microdevice applications.

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Schematics of the patterned Ti (left) and Si (right) substrates used in the current study.Each substrate is composed of an array of nine sub-patterns, one of which is left unpatterned to serve as a control, and the other eight of which consist of groove-based gratings with groove widths indicated in the schematic.
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pone-0111465-g001: Schematics of the patterned Ti (left) and Si (right) substrates used in the current study.Each substrate is composed of an array of nine sub-patterns, one of which is left unpatterned to serve as a control, and the other eight of which consist of groove-based gratings with groove widths indicated in the schematic.

Mentions: Figure 1 schematically illustrates the layouts of the patterned Ti and Si substrates used in this study, both of which share identical dimensions and patterning. One of the sub-patterns in each substrate is left unpatterned as a control, while the remainder are surface gratings consisting of periodic groove arrays with groove widths ranging from 0.5 to 50 µm, and grating pitch equal to twice the groove width (i.e., grating pitch = groove width + ridge width). Each grating sub-pattern is orthogonally-oriented with respect to its neighbors, and is surrounded by a 100 µm wide unpatterned border (thus yielding 200 µm total width of unpatterned region between neighboring sub-patterns). Use of this substrate layout provides opportunity for simultaneous evaluation of a broad feature size range within the same substrate, and therefore, within the same cell culture conditions.


Comparative endothelial cell response on topographically patterned titanium and silicon substrates with micrometer to sub-micrometer feature sizes.

Vandrangi P, Gott SC, Kozaka R, Rodgers VG, Rao MP - PLoS ONE (2014)

Schematics of the patterned Ti (left) and Si (right) substrates used in the current study.Each substrate is composed of an array of nine sub-patterns, one of which is left unpatterned to serve as a control, and the other eight of which consist of groove-based gratings with groove widths indicated in the schematic.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214724&req=5

pone-0111465-g001: Schematics of the patterned Ti (left) and Si (right) substrates used in the current study.Each substrate is composed of an array of nine sub-patterns, one of which is left unpatterned to serve as a control, and the other eight of which consist of groove-based gratings with groove widths indicated in the schematic.
Mentions: Figure 1 schematically illustrates the layouts of the patterned Ti and Si substrates used in this study, both of which share identical dimensions and patterning. One of the sub-patterns in each substrate is left unpatterned as a control, while the remainder are surface gratings consisting of periodic groove arrays with groove widths ranging from 0.5 to 50 µm, and grating pitch equal to twice the groove width (i.e., grating pitch = groove width + ridge width). Each grating sub-pattern is orthogonally-oriented with respect to its neighbors, and is surrounded by a 100 µm wide unpatterned border (thus yielding 200 µm total width of unpatterned region between neighboring sub-patterns). Use of this substrate layout provides opportunity for simultaneous evaluation of a broad feature size range within the same substrate, and therefore, within the same cell culture conditions.

Bottom Line: These specific materials are chosen due to their relevance for implantable microdevice applications, while grating-based patterns are chosen for the potential they afford for inducing elongated and aligned cellular morphologies reminiscent of the native endothelium.Moreover, we show similar trending on patterned Si substrates, albeit to a lesser extent than on comparably patterned Ti substrates.Collectively, these results suggest promise for sub-micrometer topographic patterning in general, and sub-micrometer patterning of Ti specifically, as a means for enhancing endothelialization and neovascularisation for novel implantable microdevice applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Engineering, University of California Riverside, Riverside, California, United States of America; Department of Bioengineering, University of California Riverside, Riverside, California, United States of America.

ABSTRACT
In this work, we evaluate the in vitro response of endothelial cells (EC) to variation in precisely-defined, micrometer to sub-micrometer scale topography on two different substrate materials, titanium (Ti) and silicon (Si). Both substrates possess identically-patterned surfaces composed of microfabricated, groove-based gratings with groove widths ranging from 0.5 to 50 µm, grating pitch twice the groove width, and groove depth of 1.3 µm. These specific materials are chosen due to their relevance for implantable microdevice applications, while grating-based patterns are chosen for the potential they afford for inducing elongated and aligned cellular morphologies reminiscent of the native endothelium. Using EA926 cells, a human EC variant, we show significant improvement in cellular adhesion, proliferation, morphology, and function with decreasing feature size on patterned Ti substrates. Moreover, we show similar trending on patterned Si substrates, albeit to a lesser extent than on comparably patterned Ti substrates. Collectively, these results suggest promise for sub-micrometer topographic patterning in general, and sub-micrometer patterning of Ti specifically, as a means for enhancing endothelialization and neovascularisation for novel implantable microdevice applications.

Show MeSH
Related in: MedlinePlus