Limits...
Characterization of severe fever with thrombocytopenia syndrome in rural regions of Zhejiang, China.

Zhang L, Ye L, Ojcius DM, Lou X, Wang C, feng C, Sun Y, Wang Z, Li S, Zhang Y - PLoS ONE (2014)

Bottom Line: From the constructed phylogenetic tree, it was found that all the SFTSV strains diverged into two main clusters.Two out of eight predicted linear B cell epitopes from the nucleocapsid protein showed mutations between the SFTSV strains of different clusters, but did not contribute to the binding ability of the specific SFTSV antibodies.The results also suggest that the molecular characteristics of SFTSV are associated with the geographic region and all SFTSV strains can be divided into two genotypes.

View Article: PubMed Central - PubMed

Affiliation: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang, P.R. China; Department of Civil and Environmental Engineering, University of Alberta, Edmonton, AB, Canada.

ABSTRACT
Severe fever with thrombocytopenia syndrome virus (SFTSV) infections have recently been found in rural regions of Zhejiang. A severe fever with thrombocytopenia syndrome (SFTS) surveillance and sero-epidemiological investigation was conducted in the districts with outbreaks. During the study period of 2011-2014, a total of 51 SFTSV infection cases were identified and the case fatality rate was 12% (6/51). Ninety two percent of the patients (47/51) were over 50 years of age, and 63% (32/51) of laboratory confirmed cases occurred from May to July. Nine percent (11/120) of the serum samples from local healthy people without symptoms were found to be positive for antibodies to the SFTS virus. SFTSV strains were isolated by culture using Vero, and the whole genomic sequences of two SFTSV strains (01 and Zhao) were sequenced and submitted to the GenBank. Homology analysis showed that the similarity of the target nucleocapsid gene from the SFTSV strains from different geographic areas was 94.2-100%. From the constructed phylogenetic tree, it was found that all the SFTSV strains diverged into two main clusters. Only the SFTSV strains from the Zhejiang (Daishan) region of China and the Yamaguchi, Miyazakj regions of Japan, were clustered into lineage II, consistent with both of these regions being isolated areas with similar geographic features. Two out of eight predicted linear B cell epitopes from the nucleocapsid protein showed mutations between the SFTSV strains of different clusters, but did not contribute to the binding ability of the specific SFTSV antibodies. This study confirmed that SFTSV has been circulating naturally and can cause a seasonal prevalence in Daishan, China. The results also suggest that the molecular characteristics of SFTSV are associated with the geographic region and all SFTSV strains can be divided into two genotypes.

Show MeSH

Related in: MedlinePlus

Homology comparison of the target nucleocapsid gene sequences from different SFTSV strains.A: The similarities of the target nucleocapsid gene sequences from the SFTSV strains of China (Henan, Liaoning, Shandong, Jiangsu, Hubei, Zhejiang and Anhui), Japan (Yamaguchi and Miyazakj), and South Korea (Gangwon) were 94.2–100%. B: Phylogenetic tree showed the phylogenetic positions of SFTSV strains in Zhejiang (Daishan), compared with other known SFTSV strains.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4214719&req=5

pone-0111127-g003: Homology comparison of the target nucleocapsid gene sequences from different SFTSV strains.A: The similarities of the target nucleocapsid gene sequences from the SFTSV strains of China (Henan, Liaoning, Shandong, Jiangsu, Hubei, Zhejiang and Anhui), Japan (Yamaguchi and Miyazakj), and South Korea (Gangwon) were 94.2–100%. B: Phylogenetic tree showed the phylogenetic positions of SFTSV strains in Zhejiang (Daishan), compared with other known SFTSV strains.

Mentions: Based on the results from the sequence comparisons, a phylogenetic tree was generated. According to the nucleocapsid genes sequences, SFTSV can be divided into two lineages. Interestingly, SFTSV strains from the inland of China (Anhui, Henan, Hubei, Jiangsu, Liaoning, Shandong) and South Korea were clustered into lineage I. SFTSV strains from Zhejiang (Daishan), China, and Japan, both of which are isolated regions with a similar environment, were clustered into lineage II (Figure 3).


Characterization of severe fever with thrombocytopenia syndrome in rural regions of Zhejiang, China.

Zhang L, Ye L, Ojcius DM, Lou X, Wang C, feng C, Sun Y, Wang Z, Li S, Zhang Y - PLoS ONE (2014)

Homology comparison of the target nucleocapsid gene sequences from different SFTSV strains.A: The similarities of the target nucleocapsid gene sequences from the SFTSV strains of China (Henan, Liaoning, Shandong, Jiangsu, Hubei, Zhejiang and Anhui), Japan (Yamaguchi and Miyazakj), and South Korea (Gangwon) were 94.2–100%. B: Phylogenetic tree showed the phylogenetic positions of SFTSV strains in Zhejiang (Daishan), compared with other known SFTSV strains.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214719&req=5

pone-0111127-g003: Homology comparison of the target nucleocapsid gene sequences from different SFTSV strains.A: The similarities of the target nucleocapsid gene sequences from the SFTSV strains of China (Henan, Liaoning, Shandong, Jiangsu, Hubei, Zhejiang and Anhui), Japan (Yamaguchi and Miyazakj), and South Korea (Gangwon) were 94.2–100%. B: Phylogenetic tree showed the phylogenetic positions of SFTSV strains in Zhejiang (Daishan), compared with other known SFTSV strains.
Mentions: Based on the results from the sequence comparisons, a phylogenetic tree was generated. According to the nucleocapsid genes sequences, SFTSV can be divided into two lineages. Interestingly, SFTSV strains from the inland of China (Anhui, Henan, Hubei, Jiangsu, Liaoning, Shandong) and South Korea were clustered into lineage I. SFTSV strains from Zhejiang (Daishan), China, and Japan, both of which are isolated regions with a similar environment, were clustered into lineage II (Figure 3).

Bottom Line: From the constructed phylogenetic tree, it was found that all the SFTSV strains diverged into two main clusters.Two out of eight predicted linear B cell epitopes from the nucleocapsid protein showed mutations between the SFTSV strains of different clusters, but did not contribute to the binding ability of the specific SFTSV antibodies.The results also suggest that the molecular characteristics of SFTSV are associated with the geographic region and all SFTSV strains can be divided into two genotypes.

View Article: PubMed Central - PubMed

Affiliation: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang, P.R. China; Department of Civil and Environmental Engineering, University of Alberta, Edmonton, AB, Canada.

ABSTRACT
Severe fever with thrombocytopenia syndrome virus (SFTSV) infections have recently been found in rural regions of Zhejiang. A severe fever with thrombocytopenia syndrome (SFTS) surveillance and sero-epidemiological investigation was conducted in the districts with outbreaks. During the study period of 2011-2014, a total of 51 SFTSV infection cases were identified and the case fatality rate was 12% (6/51). Ninety two percent of the patients (47/51) were over 50 years of age, and 63% (32/51) of laboratory confirmed cases occurred from May to July. Nine percent (11/120) of the serum samples from local healthy people without symptoms were found to be positive for antibodies to the SFTS virus. SFTSV strains were isolated by culture using Vero, and the whole genomic sequences of two SFTSV strains (01 and Zhao) were sequenced and submitted to the GenBank. Homology analysis showed that the similarity of the target nucleocapsid gene from the SFTSV strains from different geographic areas was 94.2-100%. From the constructed phylogenetic tree, it was found that all the SFTSV strains diverged into two main clusters. Only the SFTSV strains from the Zhejiang (Daishan) region of China and the Yamaguchi, Miyazakj regions of Japan, were clustered into lineage II, consistent with both of these regions being isolated areas with similar geographic features. Two out of eight predicted linear B cell epitopes from the nucleocapsid protein showed mutations between the SFTSV strains of different clusters, but did not contribute to the binding ability of the specific SFTSV antibodies. This study confirmed that SFTSV has been circulating naturally and can cause a seasonal prevalence in Daishan, China. The results also suggest that the molecular characteristics of SFTSV are associated with the geographic region and all SFTSV strains can be divided into two genotypes.

Show MeSH
Related in: MedlinePlus