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Characterization of severe fever with thrombocytopenia syndrome in rural regions of Zhejiang, China.

Zhang L, Ye L, Ojcius DM, Lou X, Wang C, feng C, Sun Y, Wang Z, Li S, Zhang Y - PLoS ONE (2014)

Bottom Line: From the constructed phylogenetic tree, it was found that all the SFTSV strains diverged into two main clusters.Two out of eight predicted linear B cell epitopes from the nucleocapsid protein showed mutations between the SFTSV strains of different clusters, but did not contribute to the binding ability of the specific SFTSV antibodies.The results also suggest that the molecular characteristics of SFTSV are associated with the geographic region and all SFTSV strains can be divided into two genotypes.

View Article: PubMed Central - PubMed

Affiliation: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang, P.R. China; Department of Civil and Environmental Engineering, University of Alberta, Edmonton, AB, Canada.

ABSTRACT
Severe fever with thrombocytopenia syndrome virus (SFTSV) infections have recently been found in rural regions of Zhejiang. A severe fever with thrombocytopenia syndrome (SFTS) surveillance and sero-epidemiological investigation was conducted in the districts with outbreaks. During the study period of 2011-2014, a total of 51 SFTSV infection cases were identified and the case fatality rate was 12% (6/51). Ninety two percent of the patients (47/51) were over 50 years of age, and 63% (32/51) of laboratory confirmed cases occurred from May to July. Nine percent (11/120) of the serum samples from local healthy people without symptoms were found to be positive for antibodies to the SFTS virus. SFTSV strains were isolated by culture using Vero, and the whole genomic sequences of two SFTSV strains (01 and Zhao) were sequenced and submitted to the GenBank. Homology analysis showed that the similarity of the target nucleocapsid gene from the SFTSV strains from different geographic areas was 94.2-100%. From the constructed phylogenetic tree, it was found that all the SFTSV strains diverged into two main clusters. Only the SFTSV strains from the Zhejiang (Daishan) region of China and the Yamaguchi, Miyazakj regions of Japan, were clustered into lineage II, consistent with both of these regions being isolated areas with similar geographic features. Two out of eight predicted linear B cell epitopes from the nucleocapsid protein showed mutations between the SFTSV strains of different clusters, but did not contribute to the binding ability of the specific SFTSV antibodies. This study confirmed that SFTSV has been circulating naturally and can cause a seasonal prevalence in Daishan, China. The results also suggest that the molecular characteristics of SFTSV are associated with the geographic region and all SFTSV strains can be divided into two genotypes.

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Sero-prevalence of SFTSV in healthy individuals from different villages of Daishan.A: IFA detection of IgG antibodies to SFTSV in healthy individuals. B: ELISA detection result of IgG and IgM antibodies to SFTSV in healthy individuals from different districts of Daishan.
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pone-0111127-g002: Sero-prevalence of SFTSV in healthy individuals from different villages of Daishan.A: IFA detection of IgG antibodies to SFTSV in healthy individuals. B: ELISA detection result of IgG and IgM antibodies to SFTSV in healthy individuals from different districts of Daishan.

Mentions: The sero-prevalence of SFTSV among healthy people was conducted in three rural villages in Daishan. One hundred and twenty healthy volunteers from these villages were recruited. All the study participants were local residents, and blood samples were collected from all volunteers. The age and sex distribution of the study population may have been influenced by the ability to travel of people from the countryside to cities, or a lower participation rate among older and younger people. Serum samples were tested for total antibodies (IgG and IgM) to SFTSV using a double-antigen sandwich ELISA kit, and the detection results were confirmed by the IFA. Fifty % (6/12) were found to be positive for total antibodies to SFTS virus in Dongshan, no (0/5) positive samples were positive in Daidong, and the percentage of positive samples from Gaoting was 4.9% (5/103) (Figure 2).


Characterization of severe fever with thrombocytopenia syndrome in rural regions of Zhejiang, China.

Zhang L, Ye L, Ojcius DM, Lou X, Wang C, feng C, Sun Y, Wang Z, Li S, Zhang Y - PLoS ONE (2014)

Sero-prevalence of SFTSV in healthy individuals from different villages of Daishan.A: IFA detection of IgG antibodies to SFTSV in healthy individuals. B: ELISA detection result of IgG and IgM antibodies to SFTSV in healthy individuals from different districts of Daishan.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214719&req=5

pone-0111127-g002: Sero-prevalence of SFTSV in healthy individuals from different villages of Daishan.A: IFA detection of IgG antibodies to SFTSV in healthy individuals. B: ELISA detection result of IgG and IgM antibodies to SFTSV in healthy individuals from different districts of Daishan.
Mentions: The sero-prevalence of SFTSV among healthy people was conducted in three rural villages in Daishan. One hundred and twenty healthy volunteers from these villages were recruited. All the study participants were local residents, and blood samples were collected from all volunteers. The age and sex distribution of the study population may have been influenced by the ability to travel of people from the countryside to cities, or a lower participation rate among older and younger people. Serum samples were tested for total antibodies (IgG and IgM) to SFTSV using a double-antigen sandwich ELISA kit, and the detection results were confirmed by the IFA. Fifty % (6/12) were found to be positive for total antibodies to SFTS virus in Dongshan, no (0/5) positive samples were positive in Daidong, and the percentage of positive samples from Gaoting was 4.9% (5/103) (Figure 2).

Bottom Line: From the constructed phylogenetic tree, it was found that all the SFTSV strains diverged into two main clusters.Two out of eight predicted linear B cell epitopes from the nucleocapsid protein showed mutations between the SFTSV strains of different clusters, but did not contribute to the binding ability of the specific SFTSV antibodies.The results also suggest that the molecular characteristics of SFTSV are associated with the geographic region and all SFTSV strains can be divided into two genotypes.

View Article: PubMed Central - PubMed

Affiliation: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang, P.R. China; Department of Civil and Environmental Engineering, University of Alberta, Edmonton, AB, Canada.

ABSTRACT
Severe fever with thrombocytopenia syndrome virus (SFTSV) infections have recently been found in rural regions of Zhejiang. A severe fever with thrombocytopenia syndrome (SFTS) surveillance and sero-epidemiological investigation was conducted in the districts with outbreaks. During the study period of 2011-2014, a total of 51 SFTSV infection cases were identified and the case fatality rate was 12% (6/51). Ninety two percent of the patients (47/51) were over 50 years of age, and 63% (32/51) of laboratory confirmed cases occurred from May to July. Nine percent (11/120) of the serum samples from local healthy people without symptoms were found to be positive for antibodies to the SFTS virus. SFTSV strains were isolated by culture using Vero, and the whole genomic sequences of two SFTSV strains (01 and Zhao) were sequenced and submitted to the GenBank. Homology analysis showed that the similarity of the target nucleocapsid gene from the SFTSV strains from different geographic areas was 94.2-100%. From the constructed phylogenetic tree, it was found that all the SFTSV strains diverged into two main clusters. Only the SFTSV strains from the Zhejiang (Daishan) region of China and the Yamaguchi, Miyazakj regions of Japan, were clustered into lineage II, consistent with both of these regions being isolated areas with similar geographic features. Two out of eight predicted linear B cell epitopes from the nucleocapsid protein showed mutations between the SFTSV strains of different clusters, but did not contribute to the binding ability of the specific SFTSV antibodies. This study confirmed that SFTSV has been circulating naturally and can cause a seasonal prevalence in Daishan, China. The results also suggest that the molecular characteristics of SFTSV are associated with the geographic region and all SFTSV strains can be divided into two genotypes.

Show MeSH
Related in: MedlinePlus