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Characterization of severe fever with thrombocytopenia syndrome in rural regions of Zhejiang, China.

Zhang L, Ye L, Ojcius DM, Lou X, Wang C, feng C, Sun Y, Wang Z, Li S, Zhang Y - PLoS ONE (2014)

Bottom Line: From the constructed phylogenetic tree, it was found that all the SFTSV strains diverged into two main clusters.Two out of eight predicted linear B cell epitopes from the nucleocapsid protein showed mutations between the SFTSV strains of different clusters, but did not contribute to the binding ability of the specific SFTSV antibodies.The results also suggest that the molecular characteristics of SFTSV are associated with the geographic region and all SFTSV strains can be divided into two genotypes.

View Article: PubMed Central - PubMed

Affiliation: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang, P.R. China; Department of Civil and Environmental Engineering, University of Alberta, Edmonton, AB, Canada.

ABSTRACT
Severe fever with thrombocytopenia syndrome virus (SFTSV) infections have recently been found in rural regions of Zhejiang. A severe fever with thrombocytopenia syndrome (SFTS) surveillance and sero-epidemiological investigation was conducted in the districts with outbreaks. During the study period of 2011-2014, a total of 51 SFTSV infection cases were identified and the case fatality rate was 12% (6/51). Ninety two percent of the patients (47/51) were over 50 years of age, and 63% (32/51) of laboratory confirmed cases occurred from May to July. Nine percent (11/120) of the serum samples from local healthy people without symptoms were found to be positive for antibodies to the SFTS virus. SFTSV strains were isolated by culture using Vero, and the whole genomic sequences of two SFTSV strains (01 and Zhao) were sequenced and submitted to the GenBank. Homology analysis showed that the similarity of the target nucleocapsid gene from the SFTSV strains from different geographic areas was 94.2-100%. From the constructed phylogenetic tree, it was found that all the SFTSV strains diverged into two main clusters. Only the SFTSV strains from the Zhejiang (Daishan) region of China and the Yamaguchi, Miyazakj regions of Japan, were clustered into lineage II, consistent with both of these regions being isolated areas with similar geographic features. Two out of eight predicted linear B cell epitopes from the nucleocapsid protein showed mutations between the SFTSV strains of different clusters, but did not contribute to the binding ability of the specific SFTSV antibodies. This study confirmed that SFTSV has been circulating naturally and can cause a seasonal prevalence in Daishan, China. The results also suggest that the molecular characteristics of SFTSV are associated with the geographic region and all SFTSV strains can be divided into two genotypes.

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Related in: MedlinePlus

Surveillance results for SFTS in Daishan, Zhejiang Province.A: Age distribution of diagnosed SFTS patients in Daishan from 2011 to 2014. B: Seasonality distribution of identified SFTSV infection cases in Daishan from 2011 to 2013.
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pone-0111127-g001: Surveillance results for SFTS in Daishan, Zhejiang Province.A: Age distribution of diagnosed SFTS patients in Daishan from 2011 to 2014. B: Seasonality distribution of identified SFTSV infection cases in Daishan from 2011 to 2013.

Mentions: A total of fifty-one SFTSV infection cases were identified in Daishan during the study period of 2011–2014. The major clinical symptoms included fever, thrombocytopenia, and leukocytopenia. Among all the subjects with SFTS, 45% (23/51) were male and 55% (28/51) were female, and the rate of fatality was 12% (6/51). The ages of the patients ranged from 40–85 years, and 92% (47/51) of the patients were over 50 years of age. Thirty nine out of 51 (76%) patients were local farmers living in wooden and hilly areas. There were also patients who had the professions of teacher, fishermen, etc. Though the ticks were widely distributed in the patients’ living environment, only 14% (7/51) of the patients stated that they had been bitten by ticks before the onset of the disease. Through the analysis of the SFTS surveillance data from 2011–2013, most of the laboratory-confirmed cases 63% (32/51) happened from May to July. In addition, 4% (2/51) cases occurred in March, 2% (1/51) occurred in April, 2% (1/51) occurred in August, and 2% (1/51) occurred in October (Figure 1).


Characterization of severe fever with thrombocytopenia syndrome in rural regions of Zhejiang, China.

Zhang L, Ye L, Ojcius DM, Lou X, Wang C, feng C, Sun Y, Wang Z, Li S, Zhang Y - PLoS ONE (2014)

Surveillance results for SFTS in Daishan, Zhejiang Province.A: Age distribution of diagnosed SFTS patients in Daishan from 2011 to 2014. B: Seasonality distribution of identified SFTSV infection cases in Daishan from 2011 to 2013.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214719&req=5

pone-0111127-g001: Surveillance results for SFTS in Daishan, Zhejiang Province.A: Age distribution of diagnosed SFTS patients in Daishan from 2011 to 2014. B: Seasonality distribution of identified SFTSV infection cases in Daishan from 2011 to 2013.
Mentions: A total of fifty-one SFTSV infection cases were identified in Daishan during the study period of 2011–2014. The major clinical symptoms included fever, thrombocytopenia, and leukocytopenia. Among all the subjects with SFTS, 45% (23/51) were male and 55% (28/51) were female, and the rate of fatality was 12% (6/51). The ages of the patients ranged from 40–85 years, and 92% (47/51) of the patients were over 50 years of age. Thirty nine out of 51 (76%) patients were local farmers living in wooden and hilly areas. There were also patients who had the professions of teacher, fishermen, etc. Though the ticks were widely distributed in the patients’ living environment, only 14% (7/51) of the patients stated that they had been bitten by ticks before the onset of the disease. Through the analysis of the SFTS surveillance data from 2011–2013, most of the laboratory-confirmed cases 63% (32/51) happened from May to July. In addition, 4% (2/51) cases occurred in March, 2% (1/51) occurred in April, 2% (1/51) occurred in August, and 2% (1/51) occurred in October (Figure 1).

Bottom Line: From the constructed phylogenetic tree, it was found that all the SFTSV strains diverged into two main clusters.Two out of eight predicted linear B cell epitopes from the nucleocapsid protein showed mutations between the SFTSV strains of different clusters, but did not contribute to the binding ability of the specific SFTSV antibodies.The results also suggest that the molecular characteristics of SFTSV are associated with the geographic region and all SFTSV strains can be divided into two genotypes.

View Article: PubMed Central - PubMed

Affiliation: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang, P.R. China; Department of Civil and Environmental Engineering, University of Alberta, Edmonton, AB, Canada.

ABSTRACT
Severe fever with thrombocytopenia syndrome virus (SFTSV) infections have recently been found in rural regions of Zhejiang. A severe fever with thrombocytopenia syndrome (SFTS) surveillance and sero-epidemiological investigation was conducted in the districts with outbreaks. During the study period of 2011-2014, a total of 51 SFTSV infection cases were identified and the case fatality rate was 12% (6/51). Ninety two percent of the patients (47/51) were over 50 years of age, and 63% (32/51) of laboratory confirmed cases occurred from May to July. Nine percent (11/120) of the serum samples from local healthy people without symptoms were found to be positive for antibodies to the SFTS virus. SFTSV strains were isolated by culture using Vero, and the whole genomic sequences of two SFTSV strains (01 and Zhao) were sequenced and submitted to the GenBank. Homology analysis showed that the similarity of the target nucleocapsid gene from the SFTSV strains from different geographic areas was 94.2-100%. From the constructed phylogenetic tree, it was found that all the SFTSV strains diverged into two main clusters. Only the SFTSV strains from the Zhejiang (Daishan) region of China and the Yamaguchi, Miyazakj regions of Japan, were clustered into lineage II, consistent with both of these regions being isolated areas with similar geographic features. Two out of eight predicted linear B cell epitopes from the nucleocapsid protein showed mutations between the SFTSV strains of different clusters, but did not contribute to the binding ability of the specific SFTSV antibodies. This study confirmed that SFTSV has been circulating naturally and can cause a seasonal prevalence in Daishan, China. The results also suggest that the molecular characteristics of SFTSV are associated with the geographic region and all SFTSV strains can be divided into two genotypes.

Show MeSH
Related in: MedlinePlus