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Molecular epidemiology of HIV-1 in Jilin province, northeastern China: emergence of a new CRF07_BC transmission cluster and intersubtype recombinants.

Li X, Zang X, Ning C, Feng Y, Xie C, He X, Takebe Y, Sun L, Guo Q, Xing H, Kalish ML, Shao Y - PLoS ONE (2014)

Bottom Line: Among 11 different recombinants identified, 10 contained portions of gene regions from the CRF01_AE lineage.Our study presents a molecular epidemiologic investigation describing the complex structure of HIV-1 strains co-circulating in Jilin province.The results highlight the critical importance of continuous monitoring of HIV-infections, along with detailed socio-demographic data, in order to design appropriate prevention measures to limit the spread of new HIV infections.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China, and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Zhejiang, China; HIV Molecular Epidemiology and Virology Research Group, The State Key Laboratory of Virology, Wuhan Institute of Virology, University of Chinese Academy of Sciences, Wuhan, Hubei, China.

ABSTRACT

Objective: To investigate the HIV-1 molecular epidemiology among newly diagnosed HIV-1 infected persons living in the Jilin province of northeastern China.

Methods: Plasma samples from 189 newly diagnosed HIV-1 infected patients were collected between June 2010 and August 2011 from all nine cities of Jilin province. HIV-1 nucleotide sequences of gag P17-P24 and env C2-C4 gene regions were amplified using a multiplex RT-PCR method and sequenced. Phylogenetic and recombination analyses were used to determine the HIV-1 genotypes.

Results: Based on all sequences generated, the subtype/CFR distribution was as follows: CRF01_AE (58.1%), CRF07_BC (13.2%), subtype B' (13.2%), recombinant viruses (8.1%), subtype B (3.7%), CRF02_AG (2.9%), subtype C (0.7%). In addition to finding CRF01_AE strains from previously reported transmission clusters 1, 4 and 5, a new transmission cluster was described within the CRF07_BC radiation. Among 11 different recombinants identified, 10 contained portions of gene regions from the CRF01_AE lineage. CRF02_AG was found to form a transmission cluster of 4 in local Jilin residents.

Conclusions: Our study presents a molecular epidemiologic investigation describing the complex structure of HIV-1 strains co-circulating in Jilin province. The results highlight the critical importance of continuous monitoring of HIV-infections, along with detailed socio-demographic data, in order to design appropriate prevention measures to limit the spread of new HIV infections.

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Maps of the study regions and HIV-1 genotype distribution of each geographical region in Jilin province of northeastern China.At the top-left corner of the figure is map of China (A), Jilin province of northeastern China is marked in gray. The map on the right of the figure represents Jilin province of northeastern China (B), the geographical location of the nine regions within Jilin province and the numbers of samples with genotypes from each region are shown. Pie charts show the distribution of HIV-1 genotypes in each region of Jilin province of northeastern China. The symbols representing different HIV-1 genotypes are shown at the left of the figure.
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pone-0110738-g003: Maps of the study regions and HIV-1 genotype distribution of each geographical region in Jilin province of northeastern China.At the top-left corner of the figure is map of China (A), Jilin province of northeastern China is marked in gray. The map on the right of the figure represents Jilin province of northeastern China (B), the geographical location of the nine regions within Jilin province and the numbers of samples with genotypes from each region are shown. Pie charts show the distribution of HIV-1 genotypes in each region of Jilin province of northeastern China. The symbols representing different HIV-1 genotypes are shown at the left of the figure.

Mentions: In the city of Jilin, heterosexual (9/19, 47.4%) and FPD (5/19, 26.3%) were the two major risk groups for HIV-infections with subtype B’ (8/19, 42.1%), followed by CRF01_AE (4/19, 21.1%), URFs (4/19, 21.1%) and CRF07_BC (3/19, 15.8%). In Yanbian Korean Autonomous Prefecture, the major risk group was heterosexual transmission (10/11, 90.9%), which was comprised of subtype B’ (3/11, 27.3%), CRF02_AG (3/11, 27.3%), CRF01_AE (2/11, 18.2%), recombinant viruses (2/11, 18.2%) and CRF07-1 (1/11, 9.1%). The sampling size in the remaining cities was very small (Table 1, Fig. 3).


Molecular epidemiology of HIV-1 in Jilin province, northeastern China: emergence of a new CRF07_BC transmission cluster and intersubtype recombinants.

Li X, Zang X, Ning C, Feng Y, Xie C, He X, Takebe Y, Sun L, Guo Q, Xing H, Kalish ML, Shao Y - PLoS ONE (2014)

Maps of the study regions and HIV-1 genotype distribution of each geographical region in Jilin province of northeastern China.At the top-left corner of the figure is map of China (A), Jilin province of northeastern China is marked in gray. The map on the right of the figure represents Jilin province of northeastern China (B), the geographical location of the nine regions within Jilin province and the numbers of samples with genotypes from each region are shown. Pie charts show the distribution of HIV-1 genotypes in each region of Jilin province of northeastern China. The symbols representing different HIV-1 genotypes are shown at the left of the figure.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214716&req=5

pone-0110738-g003: Maps of the study regions and HIV-1 genotype distribution of each geographical region in Jilin province of northeastern China.At the top-left corner of the figure is map of China (A), Jilin province of northeastern China is marked in gray. The map on the right of the figure represents Jilin province of northeastern China (B), the geographical location of the nine regions within Jilin province and the numbers of samples with genotypes from each region are shown. Pie charts show the distribution of HIV-1 genotypes in each region of Jilin province of northeastern China. The symbols representing different HIV-1 genotypes are shown at the left of the figure.
Mentions: In the city of Jilin, heterosexual (9/19, 47.4%) and FPD (5/19, 26.3%) were the two major risk groups for HIV-infections with subtype B’ (8/19, 42.1%), followed by CRF01_AE (4/19, 21.1%), URFs (4/19, 21.1%) and CRF07_BC (3/19, 15.8%). In Yanbian Korean Autonomous Prefecture, the major risk group was heterosexual transmission (10/11, 90.9%), which was comprised of subtype B’ (3/11, 27.3%), CRF02_AG (3/11, 27.3%), CRF01_AE (2/11, 18.2%), recombinant viruses (2/11, 18.2%) and CRF07-1 (1/11, 9.1%). The sampling size in the remaining cities was very small (Table 1, Fig. 3).

Bottom Line: Among 11 different recombinants identified, 10 contained portions of gene regions from the CRF01_AE lineage.Our study presents a molecular epidemiologic investigation describing the complex structure of HIV-1 strains co-circulating in Jilin province.The results highlight the critical importance of continuous monitoring of HIV-infections, along with detailed socio-demographic data, in order to design appropriate prevention measures to limit the spread of new HIV infections.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China, and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Zhejiang, China; HIV Molecular Epidemiology and Virology Research Group, The State Key Laboratory of Virology, Wuhan Institute of Virology, University of Chinese Academy of Sciences, Wuhan, Hubei, China.

ABSTRACT

Objective: To investigate the HIV-1 molecular epidemiology among newly diagnosed HIV-1 infected persons living in the Jilin province of northeastern China.

Methods: Plasma samples from 189 newly diagnosed HIV-1 infected patients were collected between June 2010 and August 2011 from all nine cities of Jilin province. HIV-1 nucleotide sequences of gag P17-P24 and env C2-C4 gene regions were amplified using a multiplex RT-PCR method and sequenced. Phylogenetic and recombination analyses were used to determine the HIV-1 genotypes.

Results: Based on all sequences generated, the subtype/CFR distribution was as follows: CRF01_AE (58.1%), CRF07_BC (13.2%), subtype B' (13.2%), recombinant viruses (8.1%), subtype B (3.7%), CRF02_AG (2.9%), subtype C (0.7%). In addition to finding CRF01_AE strains from previously reported transmission clusters 1, 4 and 5, a new transmission cluster was described within the CRF07_BC radiation. Among 11 different recombinants identified, 10 contained portions of gene regions from the CRF01_AE lineage. CRF02_AG was found to form a transmission cluster of 4 in local Jilin residents.

Conclusions: Our study presents a molecular epidemiologic investigation describing the complex structure of HIV-1 strains co-circulating in Jilin province. The results highlight the critical importance of continuous monitoring of HIV-infections, along with detailed socio-demographic data, in order to design appropriate prevention measures to limit the spread of new HIV infections.

Show MeSH
Related in: MedlinePlus